Metallothionein in human thrombocyte precursors, CD61+ megakaryocytes
The in vitro biosynthesis of metallothionein (MT) was investigated in thrombocyte precursors (megakaryocytes) isolated from human cord blood. Biosynthesis and induction of MT in magnetic cell sorting-separated CD61(+) megakaryocytes was confirmed by immunohistochemical staining using monoclonal mous...
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| Format: | Article |
| Language: | English |
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Springer Netherlands
2008
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| Online Access: | http://irep.iium.edu.my/12469/ http://irep.iium.edu.my/12469/1/fulltext.pdf |
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| author | Rahman, Mohammad Tariqur De Ley, Marc |
| author_facet | Rahman, Mohammad Tariqur De Ley, Marc |
| author_sort | Rahman, Mohammad Tariqur |
| building | IIUM Repository |
| collection | Online Access |
| description | The in vitro biosynthesis of metallothionein (MT) was investigated in thrombocyte precursors (megakaryocytes) isolated from human cord blood. Biosynthesis and induction of MT in magnetic cell sorting-separated CD61(+) megakaryocytes was confirmed by immunohistochemical staining using monoclonal mouse anti-MT. The presence of MT was detected both in the nuclear and in the cytoplasmic post-treatment with 100 micromol/L of zinc supplement. Seven isoform-specific mRNAs namely, MT-1A, MT-1B, MT-1E, MT-1G, MT-1H, MT-1X, and MT-2A were detected in the similar area. Using RT-PCR, in vitro upregulation/induction of total MT transcripts was observed in CD61(+) cells at 48 h cell populations left untreated with zinc. |
| first_indexed | 2025-11-14T14:46:05Z |
| format | Article |
| id | iium-12469 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T14:46:05Z |
| publishDate | 2008 |
| publisher | Springer Netherlands |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-124692012-12-06T23:54:10Z http://irep.iium.edu.my/12469/ Metallothionein in human thrombocyte precursors, CD61+ megakaryocytes Rahman, Mohammad Tariqur De Ley, Marc QH301 Biology The in vitro biosynthesis of metallothionein (MT) was investigated in thrombocyte precursors (megakaryocytes) isolated from human cord blood. Biosynthesis and induction of MT in magnetic cell sorting-separated CD61(+) megakaryocytes was confirmed by immunohistochemical staining using monoclonal mouse anti-MT. The presence of MT was detected both in the nuclear and in the cytoplasmic post-treatment with 100 micromol/L of zinc supplement. Seven isoform-specific mRNAs namely, MT-1A, MT-1B, MT-1E, MT-1G, MT-1H, MT-1X, and MT-2A were detected in the similar area. Using RT-PCR, in vitro upregulation/induction of total MT transcripts was observed in CD61(+) cells at 48 h cell populations left untreated with zinc. Springer Netherlands 2008 Article PeerReviewed application/pdf en http://irep.iium.edu.my/12469/1/fulltext.pdf Rahman, Mohammad Tariqur and De Ley, Marc (2008) Metallothionein in human thrombocyte precursors, CD61+ megakaryocytes. Cell biology and toxicology, 24 (1). pp. 19-25. ISSN 0742-2091 (P), 1573-6822 (O) http://www.springerlink.com/content/997227111149j0h6/?MUD=MP 10.1007/s10565-007-9012-3 |
| spellingShingle | QH301 Biology Rahman, Mohammad Tariqur De Ley, Marc Metallothionein in human thrombocyte precursors, CD61+ megakaryocytes |
| title | Metallothionein in human thrombocyte precursors, CD61+ megakaryocytes |
| title_full | Metallothionein in human thrombocyte precursors, CD61+ megakaryocytes |
| title_fullStr | Metallothionein in human thrombocyte precursors, CD61+ megakaryocytes |
| title_full_unstemmed | Metallothionein in human thrombocyte precursors, CD61+ megakaryocytes |
| title_short | Metallothionein in human thrombocyte precursors, CD61+ megakaryocytes |
| title_sort | metallothionein in human thrombocyte precursors, cd61+ megakaryocytes |
| topic | QH301 Biology |
| url | http://irep.iium.edu.my/12469/ http://irep.iium.edu.my/12469/ http://irep.iium.edu.my/12469/ http://irep.iium.edu.my/12469/1/fulltext.pdf |