Isolation of fucoxanthin and fatty acids analysis of Padina australis and cytotoxic effect of fucoxanthin on human lung cancer (H1299) cell lines
Fucoxanthin has been successfully isolated from species of Malaysian brown seaweed, namely Padina australis. The purity of the fucoxanthin is >98% as indicated by high performance liquid chromatography analysis. This seaweed also contains a considerable amount of unsaturated fatty acids. Thirtee...
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Academic Journals
2011
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| Online Access: | http://irep.iium.edu.my/12109/ http://irep.iium.edu.my/12109/1/Jaswir_et_al_2_isolation.pdf |
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| author | Jaswir, Irwandi Noviendri, Dedi Mohd. Salleh, Hamzah Bakhtiar, M. Taher Kazuo , Miyashita |
| author_facet | Jaswir, Irwandi Noviendri, Dedi Mohd. Salleh, Hamzah Bakhtiar, M. Taher Kazuo , Miyashita |
| author_sort | Jaswir, Irwandi |
| building | IIUM Repository |
| collection | Online Access |
| description | Fucoxanthin has been successfully isolated from species of Malaysian brown seaweed, namely Padina australis. The purity of the fucoxanthin is >98% as indicated by high performance liquid chromatography analysis. This seaweed also contains a considerable amount of unsaturated fatty
acids. Thirteen fatty acids were detected with gas chromatography. However, fatty acid methyl ester (FAMEs) of eicosapentanoic acid (C20:5n-3), arachidonic acid (C20:4n-6), linoleic acid (C18:2n-6) and alpha-linolenic acid (C18:3n-3) contents of P. australis were found to be 2.06, 9.50, 6.37, and 2.83%, respectively. For saturated fatty acids, palmitic acid (C16:0) was found to be the major fatty acid with about 23.97%. Furthermore, data obtained from the methyl thiazolyl tetrazolium (MTT) assay indicated
that fucoxanthin reduced the viability of H1299 cell lines, showing an IC50 value of 2.45 mM.
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| first_indexed | 2025-11-14T14:45:23Z |
| format | Article |
| id | iium-12109 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T14:45:23Z |
| publishDate | 2011 |
| publisher | Academic Journals |
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| spelling | iium-121092021-07-09T02:48:44Z http://irep.iium.edu.my/12109/ Isolation of fucoxanthin and fatty acids analysis of Padina australis and cytotoxic effect of fucoxanthin on human lung cancer (H1299) cell lines Jaswir, Irwandi Noviendri, Dedi Mohd. Salleh, Hamzah Bakhtiar, M. Taher Kazuo , Miyashita TP248.13 Biotechnology Fucoxanthin has been successfully isolated from species of Malaysian brown seaweed, namely Padina australis. The purity of the fucoxanthin is >98% as indicated by high performance liquid chromatography analysis. This seaweed also contains a considerable amount of unsaturated fatty acids. Thirteen fatty acids were detected with gas chromatography. However, fatty acid methyl ester (FAMEs) of eicosapentanoic acid (C20:5n-3), arachidonic acid (C20:4n-6), linoleic acid (C18:2n-6) and alpha-linolenic acid (C18:3n-3) contents of P. australis were found to be 2.06, 9.50, 6.37, and 2.83%, respectively. For saturated fatty acids, palmitic acid (C16:0) was found to be the major fatty acid with about 23.97%. Furthermore, data obtained from the methyl thiazolyl tetrazolium (MTT) assay indicated that fucoxanthin reduced the viability of H1299 cell lines, showing an IC50 value of 2.45 mM. Academic Journals 2011-12-16 Article PeerReviewed application/pdf en http://irep.iium.edu.my/12109/1/Jaswir_et_al_2_isolation.pdf Jaswir, Irwandi and Noviendri, Dedi and Mohd. Salleh, Hamzah and Bakhtiar, M. Taher and Kazuo , Miyashita (2011) Isolation of fucoxanthin and fatty acids analysis of Padina australis and cytotoxic effect of fucoxanthin on human lung cancer (H1299) cell lines. African Journal of Biotechnology, 10 (81). pp. 18855-18862. ISSN 1684–5315 http://www.academicjournals.org/AJB DOI: 10.5897/AJB11.2765 |
| spellingShingle | TP248.13 Biotechnology Jaswir, Irwandi Noviendri, Dedi Mohd. Salleh, Hamzah Bakhtiar, M. Taher Kazuo , Miyashita Isolation of fucoxanthin and fatty acids analysis of Padina australis and cytotoxic effect of fucoxanthin on human lung cancer (H1299) cell lines |
| title | Isolation of fucoxanthin and fatty acids analysis of Padina australis and cytotoxic effect of fucoxanthin on human lung cancer (H1299) cell lines |
| title_full | Isolation of fucoxanthin and fatty acids analysis of Padina australis and cytotoxic effect of fucoxanthin on human lung cancer (H1299) cell lines |
| title_fullStr | Isolation of fucoxanthin and fatty acids analysis of Padina australis and cytotoxic effect of fucoxanthin on human lung cancer (H1299) cell lines |
| title_full_unstemmed | Isolation of fucoxanthin and fatty acids analysis of Padina australis and cytotoxic effect of fucoxanthin on human lung cancer (H1299) cell lines |
| title_short | Isolation of fucoxanthin and fatty acids analysis of Padina australis and cytotoxic effect of fucoxanthin on human lung cancer (H1299) cell lines |
| title_sort | isolation of fucoxanthin and fatty acids analysis of padina australis and cytotoxic effect of fucoxanthin on human lung cancer (h1299) cell lines |
| topic | TP248.13 Biotechnology |
| url | http://irep.iium.edu.my/12109/ http://irep.iium.edu.my/12109/ http://irep.iium.edu.my/12109/ http://irep.iium.edu.my/12109/1/Jaswir_et_al_2_isolation.pdf |