A Cryopreservatlon method of Sclzaphyllurn commune local strains
Schizophyllan commune fsolates were collected from rubber plantations in Selangor and Perak area in Malaysia. Spawns were prepared using three different grains (wheat, corn and paddy). The grains were incubated for 7 days at room temperature. After the grains were fully covered with myceHa, they we...
| Main Authors: | , , |
|---|---|
| Format: | Proceeding Paper |
| Language: | English |
| Published: |
2011
|
| Subjects: | |
| Online Access: | http://irep.iium.edu.my/11991/ http://irep.iium.edu.my/11991/1/Microbiology_Congress%231.pdf |
| _version_ | 1848777384099053568 |
|---|---|
| author | Mohd Kamal, Irnada Syuhaidah Mohd Nor Rashid, Noraswati Mel, Maizirwan |
| author_facet | Mohd Kamal, Irnada Syuhaidah Mohd Nor Rashid, Noraswati Mel, Maizirwan |
| author_sort | Mohd Kamal, Irnada Syuhaidah |
| building | IIUM Repository |
| collection | Online Access |
| description | Schizophyllan commune fsolates were collected from rubber plantations in Selangor and Perak area in Malaysia. Spawns were prepared using three different grains (wheat, corn and paddy). The grains were incubated for 7 days at room temperature.
After the grains were fully covered with myceHa, they were place into a cryo-vial with three different. treatments, 10% glycerol, sterile distilled water and no cryoprotectants.
AU of the treatments were frozen in liquid nitrogen for 7 days followed by thawing (cooling) at 30°C for 30 min. The grains were inoculated onto PDA agar to observe the
viability (rate of survival) of the seeds. The radius of each grain with different treatments was measured. Results showed that all treatments showed 100% viability aft.er 48 hours and wheat wit.h no cryoprotectants showed the highest growth rate. This indicated that the grains have mycelial protective properties. In this study, a cryopreservation process was simplified as opposed to the conventional method in which 10% glycerol was used as the cryoprotectant. This study promotes an alternative method for cryopreservalion which is more economical as no cryoprotectants were required. |
| first_indexed | 2025-11-14T14:45:07Z |
| format | Proceeding Paper |
| id | iium-11991 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T14:45:07Z |
| publishDate | 2011 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-119912015-09-09T03:05:58Z http://irep.iium.edu.my/11991/ A Cryopreservatlon method of Sclzaphyllurn commune local strains Mohd Kamal, Irnada Syuhaidah Mohd Nor Rashid, Noraswati Mel, Maizirwan TP248.13 Biotechnology Schizophyllan commune fsolates were collected from rubber plantations in Selangor and Perak area in Malaysia. Spawns were prepared using three different grains (wheat, corn and paddy). The grains were incubated for 7 days at room temperature. After the grains were fully covered with myceHa, they were place into a cryo-vial with three different. treatments, 10% glycerol, sterile distilled water and no cryoprotectants. AU of the treatments were frozen in liquid nitrogen for 7 days followed by thawing (cooling) at 30°C for 30 min. The grains were inoculated onto PDA agar to observe the viability (rate of survival) of the seeds. The radius of each grain with different treatments was measured. Results showed that all treatments showed 100% viability aft.er 48 hours and wheat wit.h no cryoprotectants showed the highest growth rate. This indicated that the grains have mycelial protective properties. In this study, a cryopreservation process was simplified as opposed to the conventional method in which 10% glycerol was used as the cryoprotectant. This study promotes an alternative method for cryopreservalion which is more economical as no cryoprotectants were required. 2011-12-08 Proceeding Paper PeerReviewed application/pdf en http://irep.iium.edu.my/11991/1/Microbiology_Congress%231.pdf Mohd Kamal, Irnada Syuhaidah and Mohd Nor Rashid, Noraswati and Mel, Maizirwan (2011) A Cryopreservatlon method of Sclzaphyllurn commune local strains. In: International Congress of the Malaysian Society for Microbiology 2011, 8-11 December 2011, Penang. |
| spellingShingle | TP248.13 Biotechnology Mohd Kamal, Irnada Syuhaidah Mohd Nor Rashid, Noraswati Mel, Maizirwan A Cryopreservatlon method of Sclzaphyllurn commune local strains |
| title | A Cryopreservatlon method of Sclzaphyllurn commune local strains |
| title_full | A Cryopreservatlon method of Sclzaphyllurn commune local strains |
| title_fullStr | A Cryopreservatlon method of Sclzaphyllurn commune local strains |
| title_full_unstemmed | A Cryopreservatlon method of Sclzaphyllurn commune local strains |
| title_short | A Cryopreservatlon method of Sclzaphyllurn commune local strains |
| title_sort | cryopreservatlon method of sclzaphyllurn commune local strains |
| topic | TP248.13 Biotechnology |
| url | http://irep.iium.edu.my/11991/ http://irep.iium.edu.my/11991/1/Microbiology_Congress%231.pdf |