Media screening and optimization of lactic acid fermentation

The screening and optimization of lactic acid production using bacteria L. rhamnosus were successfully carried out in shake flask experiment. Initially eleven variables were screened using Placket Burman Design and result indicated that, the main variables that effected were glucose and peptone...

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Main Authors: Abdul Karim, Mohamed Ismail, Mel, Maizirwan, Jamal, Parveen, Mohamed Salleh, Mohamad Ramlan, Alamin, Noraini
Format: Proceeding Paper
Language:English
Published: 2005
Subjects:
Online Access:http://irep.iium.edu.my/10635/
http://irep.iium.edu.my/10635/1/BPE-33.pdf
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author Abdul Karim, Mohamed Ismail
Mel, Maizirwan
Jamal, Parveen
Mohamed Salleh, Mohamad Ramlan
Alamin, Noraini
author_facet Abdul Karim, Mohamed Ismail
Mel, Maizirwan
Jamal, Parveen
Mohamed Salleh, Mohamad Ramlan
Alamin, Noraini
author_sort Abdul Karim, Mohamed Ismail
building IIUM Repository
collection Online Access
description The screening and optimization of lactic acid production using bacteria L. rhamnosus were successfully carried out in shake flask experiment. Initially eleven variables were screened using Placket Burman Design and result indicated that, the main variables that effected were glucose and peptone. These two most significant variables were then optimized using the Central Composite Design. The correlation between those two variables was analyzed using Response Surface Methodology (RSM). The contour plot indicates that the interactions between these two variables are not much significantly different. The optimization and critical value indicate that the most ideal concentration of glucose and peptone for optimum production of lactic acid in shake flask is 9.80g/l and 9.98g/l, respectively. The maximum growth rate for these bacteria is 0.394 h-1 and the productivity is 0.630 g/g.h.
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format Proceeding Paper
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institution International Islamic University Malaysia
institution_category Local University
language English
last_indexed 2025-11-14T14:42:48Z
publishDate 2005
recordtype eprints
repository_type Digital Repository
spelling iium-106352013-05-17T07:23:09Z http://irep.iium.edu.my/10635/ Media screening and optimization of lactic acid fermentation Abdul Karim, Mohamed Ismail Mel, Maizirwan Jamal, Parveen Mohamed Salleh, Mohamad Ramlan Alamin, Noraini TP248.13 Biotechnology TP500 Fermentation industries. Beverages. Alcohol The screening and optimization of lactic acid production using bacteria L. rhamnosus were successfully carried out in shake flask experiment. Initially eleven variables were screened using Placket Burman Design and result indicated that, the main variables that effected were glucose and peptone. These two most significant variables were then optimized using the Central Composite Design. The correlation between those two variables was analyzed using Response Surface Methodology (RSM). The contour plot indicates that the interactions between these two variables are not much significantly different. The optimization and critical value indicate that the most ideal concentration of glucose and peptone for optimum production of lactic acid in shake flask is 9.80g/l and 9.98g/l, respectively. The maximum growth rate for these bacteria is 0.394 h-1 and the productivity is 0.630 g/g.h. 2005 Proceeding Paper PeerReviewed application/pdf en http://irep.iium.edu.my/10635/1/BPE-33.pdf Abdul Karim, Mohamed Ismail and Mel, Maizirwan and Jamal, Parveen and Mohamed Salleh, Mohamad Ramlan and Alamin, Noraini (2005) Media screening and optimization of lactic acid fermentation. In: 2nd International Conference on Chemical and Bioprocess Engineering (ICCBpE-SOMChE 2005), 8-10 Dec 2005, Kota Kinabalu, Sabah.
spellingShingle TP248.13 Biotechnology
TP500 Fermentation industries. Beverages. Alcohol
Abdul Karim, Mohamed Ismail
Mel, Maizirwan
Jamal, Parveen
Mohamed Salleh, Mohamad Ramlan
Alamin, Noraini
Media screening and optimization of lactic acid fermentation
title Media screening and optimization of lactic acid fermentation
title_full Media screening and optimization of lactic acid fermentation
title_fullStr Media screening and optimization of lactic acid fermentation
title_full_unstemmed Media screening and optimization of lactic acid fermentation
title_short Media screening and optimization of lactic acid fermentation
title_sort media screening and optimization of lactic acid fermentation
topic TP248.13 Biotechnology
TP500 Fermentation industries. Beverages. Alcohol
url http://irep.iium.edu.my/10635/
http://irep.iium.edu.my/10635/1/BPE-33.pdf