Renoprotective effect of long acting thioredoxin by modulating oxidative stress and macrophage migration inhibitory factor against rhabdomyolysis-Associated acute kidney injury
Rhabdomyolysis-Associated acute kidney injury (AKI) is a serious life-Threatening condition. As such, more effective strategies are needed for its prevention. Thioredoxin-1 (Trx), a redox-Active and macrophage migration inhibitory factor (MIF) modulating protein, has a short retention time in the bl...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Journal Article |
| Published: |
2015
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| Online Access: | http://hdl.handle.net/20.500.11937/9945 |
| _version_ | 1848746095936536576 |
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| author | Nishida, K. Watanabe, H. Ogaki, S. Kodama, A. Tanaka, R. Imafuku, T. Ishima, Y. Toyoda, M. Kondoh, M. Wu, Q. Fukagawa, M. Otagiri, M. Maruyama, T. Chuang, Victor |
| author_facet | Nishida, K. Watanabe, H. Ogaki, S. Kodama, A. Tanaka, R. Imafuku, T. Ishima, Y. Toyoda, M. Kondoh, M. Wu, Q. Fukagawa, M. Otagiri, M. Maruyama, T. Chuang, Victor |
| author_sort | Nishida, K. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | Rhabdomyolysis-Associated acute kidney injury (AKI) is a serious life-Threatening condition. As such, more effective strategies are needed for its prevention. Thioredoxin-1 (Trx), a redox-Active and macrophage migration inhibitory factor (MIF) modulating protein, has a short retention time in the blood. We examined the renoprotective effect of long acting Trx that was genetically fused with human serum albumin (HSA-Trx) against glycerol-induced AKI. An intravenous HSA-Trx pre-Treatment attenuated the glycerol-induced decline in renal function, compared to a PBS, HSA or Trx alone. HSA-Trx caused a reduction in the tubular injuries and in the number of apoptosis-positive tubular cells. Renal superoxide, 8-hydroxy deoxyguanosine, nitrotyrosine and the plasma Cys34-cysteinylated albumin were clearly suppressed by the HSA-Trx treatment. Prior to decreasing TNF-a and IL-6, HSA-Trx suppressed an increase of plasma MIF level. In LLC-PK1 cells, HSA-Trx decreased the level of reactive oxygen species and lactate dehydrogenase release induced by myoglobin. HSA-Trx treatment resulted in a threefold increase in the survival of lethal glycerol-Treated mice. The post-Administration of HSA-Trx at 1 and 3â €‰hr after glycerol injection exerted a significant renoprotective effect. These results suggest HSA-Trx has potential for use in the treatment of rhabdomyolysis-Associated AKI via its extended effects of modulating oxidative stress and MIF. |
| first_indexed | 2025-11-14T06:27:49Z |
| format | Journal Article |
| id | curtin-20.500.11937-9945 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T06:27:49Z |
| publishDate | 2015 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-99452017-09-13T14:53:17Z Renoprotective effect of long acting thioredoxin by modulating oxidative stress and macrophage migration inhibitory factor against rhabdomyolysis-Associated acute kidney injury Nishida, K. Watanabe, H. Ogaki, S. Kodama, A. Tanaka, R. Imafuku, T. Ishima, Y. Toyoda, M. Kondoh, M. Wu, Q. Fukagawa, M. Otagiri, M. Maruyama, T. Chuang, Victor Rhabdomyolysis-Associated acute kidney injury (AKI) is a serious life-Threatening condition. As such, more effective strategies are needed for its prevention. Thioredoxin-1 (Trx), a redox-Active and macrophage migration inhibitory factor (MIF) modulating protein, has a short retention time in the blood. We examined the renoprotective effect of long acting Trx that was genetically fused with human serum albumin (HSA-Trx) against glycerol-induced AKI. An intravenous HSA-Trx pre-Treatment attenuated the glycerol-induced decline in renal function, compared to a PBS, HSA or Trx alone. HSA-Trx caused a reduction in the tubular injuries and in the number of apoptosis-positive tubular cells. Renal superoxide, 8-hydroxy deoxyguanosine, nitrotyrosine and the plasma Cys34-cysteinylated albumin were clearly suppressed by the HSA-Trx treatment. Prior to decreasing TNF-a and IL-6, HSA-Trx suppressed an increase of plasma MIF level. In LLC-PK1 cells, HSA-Trx decreased the level of reactive oxygen species and lactate dehydrogenase release induced by myoglobin. HSA-Trx treatment resulted in a threefold increase in the survival of lethal glycerol-Treated mice. The post-Administration of HSA-Trx at 1 and 3â €‰hr after glycerol injection exerted a significant renoprotective effect. These results suggest HSA-Trx has potential for use in the treatment of rhabdomyolysis-Associated AKI via its extended effects of modulating oxidative stress and MIF. 2015 Journal Article http://hdl.handle.net/20.500.11937/9945 10.1038/srep14471 unknown |
| spellingShingle | Nishida, K. Watanabe, H. Ogaki, S. Kodama, A. Tanaka, R. Imafuku, T. Ishima, Y. Toyoda, M. Kondoh, M. Wu, Q. Fukagawa, M. Otagiri, M. Maruyama, T. Chuang, Victor Renoprotective effect of long acting thioredoxin by modulating oxidative stress and macrophage migration inhibitory factor against rhabdomyolysis-Associated acute kidney injury |
| title | Renoprotective effect of long acting thioredoxin by modulating oxidative stress and macrophage migration inhibitory factor against rhabdomyolysis-Associated acute kidney injury |
| title_full | Renoprotective effect of long acting thioredoxin by modulating oxidative stress and macrophage migration inhibitory factor against rhabdomyolysis-Associated acute kidney injury |
| title_fullStr | Renoprotective effect of long acting thioredoxin by modulating oxidative stress and macrophage migration inhibitory factor against rhabdomyolysis-Associated acute kidney injury |
| title_full_unstemmed | Renoprotective effect of long acting thioredoxin by modulating oxidative stress and macrophage migration inhibitory factor against rhabdomyolysis-Associated acute kidney injury |
| title_short | Renoprotective effect of long acting thioredoxin by modulating oxidative stress and macrophage migration inhibitory factor against rhabdomyolysis-Associated acute kidney injury |
| title_sort | renoprotective effect of long acting thioredoxin by modulating oxidative stress and macrophage migration inhibitory factor against rhabdomyolysis-associated acute kidney injury |
| url | http://hdl.handle.net/20.500.11937/9945 |