Next-generation re-sequencing as a tool for rapid bioinformatic screening of presence and absence of genes and accessory chromosomes across isolates of Zymoseptoria tritici.
The wheat pathogen Zymoseptoria tritici possesses a large number of accessory chromosomes that may be present or absent in its genome. The genome of the reference isolate IPO323 has been assembled to a very high standard and contains 21 full length chromosome sequences, 8 of which represent accessor...
| Main Authors: | , , , , , |
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| Format: | Journal Article |
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Academic Press
2015
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| Online Access: | http://hdl.handle.net/20.500.11937/9842 |
| _version_ | 1848746067109085184 |
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| author | McDonald, M. Williams, A. Milgate, A. Pattemore, J. Solomon, P. Hane, James |
| author_facet | McDonald, M. Williams, A. Milgate, A. Pattemore, J. Solomon, P. Hane, James |
| author_sort | McDonald, M. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | The wheat pathogen Zymoseptoria tritici possesses a large number of accessory chromosomes that may be present or absent in its genome. The genome of the reference isolate IPO323 has been assembled to a very high standard and contains 21 full length chromosome sequences, 8 of which represent accessory chromosomes. The IPO323 reference, when combined with low-cost next-generation sequencing and bioinformatics, can be used as a powerful tool to assess the presence or absence of accessory chromosomes. We present an outline of a range of bioinformatics techniques that can be applied to the analysis of presence–absence variation among accessory chromosomes across 13 novel isolates of Z. tritici. |
| first_indexed | 2025-11-14T06:27:21Z |
| format | Journal Article |
| id | curtin-20.500.11937-9842 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T06:27:21Z |
| publishDate | 2015 |
| publisher | Academic Press |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-98422017-09-13T14:50:55Z Next-generation re-sequencing as a tool for rapid bioinformatic screening of presence and absence of genes and accessory chromosomes across isolates of Zymoseptoria tritici. McDonald, M. Williams, A. Milgate, A. Pattemore, J. Solomon, P. Hane, James Accessory chromosome Zymoseptoria tritici Presence–absence variation Next-generation sequencing Comparative genomics The wheat pathogen Zymoseptoria tritici possesses a large number of accessory chromosomes that may be present or absent in its genome. The genome of the reference isolate IPO323 has been assembled to a very high standard and contains 21 full length chromosome sequences, 8 of which represent accessory chromosomes. The IPO323 reference, when combined with low-cost next-generation sequencing and bioinformatics, can be used as a powerful tool to assess the presence or absence of accessory chromosomes. We present an outline of a range of bioinformatics techniques that can be applied to the analysis of presence–absence variation among accessory chromosomes across 13 novel isolates of Z. tritici. 2015 Journal Article http://hdl.handle.net/20.500.11937/9842 10.1016/j.fgb.2015.04.012 Academic Press unknown |
| spellingShingle | Accessory chromosome Zymoseptoria tritici Presence–absence variation Next-generation sequencing Comparative genomics McDonald, M. Williams, A. Milgate, A. Pattemore, J. Solomon, P. Hane, James Next-generation re-sequencing as a tool for rapid bioinformatic screening of presence and absence of genes and accessory chromosomes across isolates of Zymoseptoria tritici. |
| title | Next-generation re-sequencing as a tool for rapid bioinformatic screening of presence and absence of genes and accessory chromosomes across isolates of Zymoseptoria tritici. |
| title_full | Next-generation re-sequencing as a tool for rapid bioinformatic screening of presence and absence of genes and accessory chromosomes across isolates of Zymoseptoria tritici. |
| title_fullStr | Next-generation re-sequencing as a tool for rapid bioinformatic screening of presence and absence of genes and accessory chromosomes across isolates of Zymoseptoria tritici. |
| title_full_unstemmed | Next-generation re-sequencing as a tool for rapid bioinformatic screening of presence and absence of genes and accessory chromosomes across isolates of Zymoseptoria tritici. |
| title_short | Next-generation re-sequencing as a tool for rapid bioinformatic screening of presence and absence of genes and accessory chromosomes across isolates of Zymoseptoria tritici. |
| title_sort | next-generation re-sequencing as a tool for rapid bioinformatic screening of presence and absence of genes and accessory chromosomes across isolates of zymoseptoria tritici. |
| topic | Accessory chromosome Zymoseptoria tritici Presence–absence variation Next-generation sequencing Comparative genomics |
| url | http://hdl.handle.net/20.500.11937/9842 |