Antimicrobial activity of crude epicarp and seed extracts from mature avocado fruit (Persea americana) of three cultivars

The epicarp and seed of Persea Americana Mill. var. Hass (Lauraceae), Persea Americana Mill. var. Shepard, and Persea americana Mill. var Fuerte cultivars of mature avocados (n = 3) were ground separately and extracted with both absolute ethanol and distilled water. Extracts were analyzed for antimi...

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Main Authors: Raymond Chia, T., Dykes, Gary
Format: Journal Article
Published: Informa Healthcare 2010
Online Access:http://hdl.handle.net/20.500.11937/9822
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author Raymond Chia, T.
Dykes, Gary
author_facet Raymond Chia, T.
Dykes, Gary
author_sort Raymond Chia, T.
building Curtin Institutional Repository
collection Online Access
description The epicarp and seed of Persea Americana Mill. var. Hass (Lauraceae), Persea Americana Mill. var. Shepard, and Persea americana Mill. var Fuerte cultivars of mature avocados (n = 3) were ground separately and extracted with both absolute ethanol and distilled water. Extracts were analyzed for antimicrobial activity using the microtiter broth microdilution assay against four Gram-positive bacteria, six Gram-negative bacteria, and one yeast. Antimicrobial activity against two molds was determined by the hole plate method. The ethanol extracts showed antimicrobial activity (104.2-416.7 µg/mL) toward both Gram-positive and Gram-negative bacteria (except Escherichia coli), while inhibition of the water extracts was only observed for Listeria monocytogenes (93.8-375.0 µg/mL) and Staphylococcus epidermidis (354.2 µg/mL). The minimum concentration required to inhibit Zygosaccharomyces bailii was 500 µg/mL for the ethanol extracts, while no inhibition was observed for the water extracts. No inhibition by either ethanol or water extracts was observed against Penicillium spp. and Aspergillus flavus. © 2010 Informa UK Ltd.
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spelling curtin-20.500.11937-98222017-09-13T14:52:45Z Antimicrobial activity of crude epicarp and seed extracts from mature avocado fruit (Persea americana) of three cultivars Raymond Chia, T. Dykes, Gary The epicarp and seed of Persea Americana Mill. var. Hass (Lauraceae), Persea Americana Mill. var. Shepard, and Persea americana Mill. var Fuerte cultivars of mature avocados (n = 3) were ground separately and extracted with both absolute ethanol and distilled water. Extracts were analyzed for antimicrobial activity using the microtiter broth microdilution assay against four Gram-positive bacteria, six Gram-negative bacteria, and one yeast. Antimicrobial activity against two molds was determined by the hole plate method. The ethanol extracts showed antimicrobial activity (104.2-416.7 µg/mL) toward both Gram-positive and Gram-negative bacteria (except Escherichia coli), while inhibition of the water extracts was only observed for Listeria monocytogenes (93.8-375.0 µg/mL) and Staphylococcus epidermidis (354.2 µg/mL). The minimum concentration required to inhibit Zygosaccharomyces bailii was 500 µg/mL for the ethanol extracts, while no inhibition was observed for the water extracts. No inhibition by either ethanol or water extracts was observed against Penicillium spp. and Aspergillus flavus. © 2010 Informa UK Ltd. 2010 Journal Article http://hdl.handle.net/20.500.11937/9822 10.3109/13880200903273922 Informa Healthcare unknown
spellingShingle Raymond Chia, T.
Dykes, Gary
Antimicrobial activity of crude epicarp and seed extracts from mature avocado fruit (Persea americana) of three cultivars
title Antimicrobial activity of crude epicarp and seed extracts from mature avocado fruit (Persea americana) of three cultivars
title_full Antimicrobial activity of crude epicarp and seed extracts from mature avocado fruit (Persea americana) of three cultivars
title_fullStr Antimicrobial activity of crude epicarp and seed extracts from mature avocado fruit (Persea americana) of three cultivars
title_full_unstemmed Antimicrobial activity of crude epicarp and seed extracts from mature avocado fruit (Persea americana) of three cultivars
title_short Antimicrobial activity of crude epicarp and seed extracts from mature avocado fruit (Persea americana) of three cultivars
title_sort antimicrobial activity of crude epicarp and seed extracts from mature avocado fruit (persea americana) of three cultivars
url http://hdl.handle.net/20.500.11937/9822