Flies who cannot take the heat: Genome-wide gene expression analysis of temperature-sensitive lethality in an inbred line of Drosophila melanogaster
Fitness decreases associated with inbreeding depression often become more pronounced in a stressful environment. The functional genomic causes of these inbreeding-by-environment (I × E) interactions, and of inbreeding depression in general, are poorly known. To further our understanding of I × E int...
| Main Authors: | , , , |
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| Format: | Journal Article |
| Language: | English |
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2014
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| Online Access: | https://onlinelibrary.wiley.com/doi/pdfdirect/10.1111/jeb.12472 http://hdl.handle.net/20.500.11937/96877 |
| _version_ | 1848766204443885568 |
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| author | Vermeulen, C.J. Sørensen, P. Gagalova, Kristina Loeschcke, V. |
| author_facet | Vermeulen, C.J. Sørensen, P. Gagalova, Kristina Loeschcke, V. |
| author_sort | Vermeulen, C.J. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | Fitness decreases associated with inbreeding depression often become more pronounced in a stressful environment. The functional genomic causes of these inbreeding-by-environment (I × E) interactions, and of inbreeding depression in general, are poorly known. To further our understanding of I × E interactions, we performed a genome-wide gene expression study of a single inbred line that suffers from temperature-sensitive lethality. We confirmed that increased differential expression between the thermosensitive line and the control line occurs at the restrictive temperature. This demonstrates that I × E interactions in survival are reflected in similar I × E interactions at the gene expression level. To make an impression of the cellular response associated with the lethal effect, we analysed all functional annotation terms that were overrepresented among the differentially expressed genes. Some sets of differentially expressed genes function in the general stress response, and these are more likely to also be differentially expressed in other studies of inbreeding, inbreeding depression, immunity and heat stress. Other sets of differentially expressed genes are shared with studies of gene expression in inbred lines, but not studies of the response to extrinsic stress, and represent a general transcriptomic signature of inbreeding. Finally, some sets of genes have an annotation that is not reported in other studies. These we consider to be candidates for the genes harbouring the mutations responsible for the thermosensitive phenotype, as these mutations are expected to be unique to this line. These genes may also serve as candidate QTL in studies of thermal tolerance and heat resistance. |
| first_indexed | 2025-11-14T11:47:26Z |
| format | Journal Article |
| id | curtin-20.500.11937-96877 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| language | eng |
| last_indexed | 2025-11-14T11:47:26Z |
| publishDate | 2014 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-968772025-02-13T00:50:00Z Flies who cannot take the heat: Genome-wide gene expression analysis of temperature-sensitive lethality in an inbred line of Drosophila melanogaster Vermeulen, C.J. Sørensen, P. Gagalova, Kristina Loeschcke, V. conditional lethality gene expression genotype-by-environment interaction inbreeding depression mitochondrial large ribosomal subunit septate junction stressful environments thermal resistance transcriptome Animals Drosophila melanogaster Genes, Insect Genes, Lethal Genetic Association Studies Hot Temperature Inbreeding Male Phenotype Quantitative Trait Loci Stress, Physiological Transcriptome Animals Drosophila melanogaster Inbreeding Phenotype Genes, Insect Genes, Lethal Quantitative Trait Loci Male Hot Temperature Stress, Physiological Genetic Association Studies Transcriptome Fitness decreases associated with inbreeding depression often become more pronounced in a stressful environment. The functional genomic causes of these inbreeding-by-environment (I × E) interactions, and of inbreeding depression in general, are poorly known. To further our understanding of I × E interactions, we performed a genome-wide gene expression study of a single inbred line that suffers from temperature-sensitive lethality. We confirmed that increased differential expression between the thermosensitive line and the control line occurs at the restrictive temperature. This demonstrates that I × E interactions in survival are reflected in similar I × E interactions at the gene expression level. To make an impression of the cellular response associated with the lethal effect, we analysed all functional annotation terms that were overrepresented among the differentially expressed genes. Some sets of differentially expressed genes function in the general stress response, and these are more likely to also be differentially expressed in other studies of inbreeding, inbreeding depression, immunity and heat stress. Other sets of differentially expressed genes are shared with studies of gene expression in inbred lines, but not studies of the response to extrinsic stress, and represent a general transcriptomic signature of inbreeding. Finally, some sets of genes have an annotation that is not reported in other studies. These we consider to be candidates for the genes harbouring the mutations responsible for the thermosensitive phenotype, as these mutations are expected to be unique to this line. These genes may also serve as candidate QTL in studies of thermal tolerance and heat resistance. 2014 Journal Article http://hdl.handle.net/20.500.11937/96877 10.1111/jeb.12472 eng https://onlinelibrary.wiley.com/doi/pdfdirect/10.1111/jeb.12472 restricted |
| spellingShingle | conditional lethality gene expression genotype-by-environment interaction inbreeding depression mitochondrial large ribosomal subunit septate junction stressful environments thermal resistance transcriptome Animals Drosophila melanogaster Genes, Insect Genes, Lethal Genetic Association Studies Hot Temperature Inbreeding Male Phenotype Quantitative Trait Loci Stress, Physiological Transcriptome Animals Drosophila melanogaster Inbreeding Phenotype Genes, Insect Genes, Lethal Quantitative Trait Loci Male Hot Temperature Stress, Physiological Genetic Association Studies Transcriptome Vermeulen, C.J. Sørensen, P. Gagalova, Kristina Loeschcke, V. Flies who cannot take the heat: Genome-wide gene expression analysis of temperature-sensitive lethality in an inbred line of Drosophila melanogaster |
| title | Flies who cannot take the heat: Genome-wide gene expression analysis of temperature-sensitive lethality in an inbred line of Drosophila melanogaster |
| title_full | Flies who cannot take the heat: Genome-wide gene expression analysis of temperature-sensitive lethality in an inbred line of Drosophila melanogaster |
| title_fullStr | Flies who cannot take the heat: Genome-wide gene expression analysis of temperature-sensitive lethality in an inbred line of Drosophila melanogaster |
| title_full_unstemmed | Flies who cannot take the heat: Genome-wide gene expression analysis of temperature-sensitive lethality in an inbred line of Drosophila melanogaster |
| title_short | Flies who cannot take the heat: Genome-wide gene expression analysis of temperature-sensitive lethality in an inbred line of Drosophila melanogaster |
| title_sort | flies who cannot take the heat: genome-wide gene expression analysis of temperature-sensitive lethality in an inbred line of drosophila melanogaster |
| topic | conditional lethality gene expression genotype-by-environment interaction inbreeding depression mitochondrial large ribosomal subunit septate junction stressful environments thermal resistance transcriptome Animals Drosophila melanogaster Genes, Insect Genes, Lethal Genetic Association Studies Hot Temperature Inbreeding Male Phenotype Quantitative Trait Loci Stress, Physiological Transcriptome Animals Drosophila melanogaster Inbreeding Phenotype Genes, Insect Genes, Lethal Quantitative Trait Loci Male Hot Temperature Stress, Physiological Genetic Association Studies Transcriptome |
| url | https://onlinelibrary.wiley.com/doi/pdfdirect/10.1111/jeb.12472 http://hdl.handle.net/20.500.11937/96877 |