Induction of interferon-stimulated genes and cellular stress pathways by morpholinos in zebrafish
The phenotypes caused by morpholino-mediated interference of gene function in zebrafish are often not observed in the corresponding mutant(s). We took advantage of the availability of a relatively large collection of transcriptomic datasets to identify common signatures that characterize morpholino-...
| Main Authors: | , , , , |
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| Format: | Journal Article |
| Language: | English |
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2019
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| Online Access: | http://hdl.handle.net/20.500.11937/96872 |
| _version_ | 1848766203146797056 |
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| author | Lai, J.K.H. Gagalova, Kristina Kuenne, C. El-Brolosy, M.A. Stainier, D.Y.R. |
| author_facet | Lai, J.K.H. Gagalova, Kristina Kuenne, C. El-Brolosy, M.A. Stainier, D.Y.R. |
| author_sort | Lai, J.K.H. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | The phenotypes caused by morpholino-mediated interference of gene function in zebrafish are often not observed in the corresponding mutant(s). We took advantage of the availability of a relatively large collection of transcriptomic datasets to identify common signatures that characterize morpholino-injected animals (morphants). In addition to the previously reported activation of tp53 expression, we observed increased expression of the interferon-stimulated genes (ISGs), isg15 and isg20, the cell death pathway gene casp8, and other cellular stress response genes including phlda3, mdm2 and gadd45aa. Studies involving segmentation stage embryos were more likely to show upregulation of these genes. We also found that the expression of these genes could be upregulated by increasing doses of an egfl7 morpholino, or even high doses of the standard control morpholino. Thus, these data show that morpholinos can induce the expression of ISGs in zebrafish embryos and further our understanding of morpholino effects. |
| first_indexed | 2025-11-14T11:47:24Z |
| format | Journal Article |
| id | curtin-20.500.11937-96872 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| language | eng |
| last_indexed | 2025-11-14T11:47:24Z |
| publishDate | 2019 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-968722025-02-13T00:40:56Z Induction of interferon-stimulated genes and cellular stress pathways by morpholinos in zebrafish Lai, J.K.H. Gagalova, Kristina Kuenne, C. El-Brolosy, M.A. Stainier, D.Y.R. ISG Knockdown Morpholino Mutant Zebrafish p53 Animals Down-Regulation Embryo, Nonmammalian Gene Expression Profiling Gene Expression Regulation, Developmental Gene Knockdown Techniques Interferons Morpholinos Mutation Phenotype Stress, Physiological Tumor Suppressor Protein p53 Up-Regulation Zebrafish Zebrafish Proteins Embryo, Nonmammalian Animals Zebrafish Zebrafish Proteins Interferons Gene Expression Profiling Down-Regulation Gene Expression Regulation, Developmental Up-Regulation Phenotype Mutation Tumor Suppressor Protein p53 Stress, Physiological Gene Knockdown Techniques Morpholinos The phenotypes caused by morpholino-mediated interference of gene function in zebrafish are often not observed in the corresponding mutant(s). We took advantage of the availability of a relatively large collection of transcriptomic datasets to identify common signatures that characterize morpholino-injected animals (morphants). In addition to the previously reported activation of tp53 expression, we observed increased expression of the interferon-stimulated genes (ISGs), isg15 and isg20, the cell death pathway gene casp8, and other cellular stress response genes including phlda3, mdm2 and gadd45aa. Studies involving segmentation stage embryos were more likely to show upregulation of these genes. We also found that the expression of these genes could be upregulated by increasing doses of an egfl7 morpholino, or even high doses of the standard control morpholino. Thus, these data show that morpholinos can induce the expression of ISGs in zebrafish embryos and further our understanding of morpholino effects. 2019 Journal Article http://hdl.handle.net/20.500.11937/96872 10.1016/j.ydbio.2019.06.008 eng unknown |
| spellingShingle | ISG Knockdown Morpholino Mutant Zebrafish p53 Animals Down-Regulation Embryo, Nonmammalian Gene Expression Profiling Gene Expression Regulation, Developmental Gene Knockdown Techniques Interferons Morpholinos Mutation Phenotype Stress, Physiological Tumor Suppressor Protein p53 Up-Regulation Zebrafish Zebrafish Proteins Embryo, Nonmammalian Animals Zebrafish Zebrafish Proteins Interferons Gene Expression Profiling Down-Regulation Gene Expression Regulation, Developmental Up-Regulation Phenotype Mutation Tumor Suppressor Protein p53 Stress, Physiological Gene Knockdown Techniques Morpholinos Lai, J.K.H. Gagalova, Kristina Kuenne, C. El-Brolosy, M.A. Stainier, D.Y.R. Induction of interferon-stimulated genes and cellular stress pathways by morpholinos in zebrafish |
| title | Induction of interferon-stimulated genes and cellular stress pathways by morpholinos in zebrafish |
| title_full | Induction of interferon-stimulated genes and cellular stress pathways by morpholinos in zebrafish |
| title_fullStr | Induction of interferon-stimulated genes and cellular stress pathways by morpholinos in zebrafish |
| title_full_unstemmed | Induction of interferon-stimulated genes and cellular stress pathways by morpholinos in zebrafish |
| title_short | Induction of interferon-stimulated genes and cellular stress pathways by morpholinos in zebrafish |
| title_sort | induction of interferon-stimulated genes and cellular stress pathways by morpholinos in zebrafish |
| topic | ISG Knockdown Morpholino Mutant Zebrafish p53 Animals Down-Regulation Embryo, Nonmammalian Gene Expression Profiling Gene Expression Regulation, Developmental Gene Knockdown Techniques Interferons Morpholinos Mutation Phenotype Stress, Physiological Tumor Suppressor Protein p53 Up-Regulation Zebrafish Zebrafish Proteins Embryo, Nonmammalian Animals Zebrafish Zebrafish Proteins Interferons Gene Expression Profiling Down-Regulation Gene Expression Regulation, Developmental Up-Regulation Phenotype Mutation Tumor Suppressor Protein p53 Stress, Physiological Gene Knockdown Techniques Morpholinos |
| url | http://hdl.handle.net/20.500.11937/96872 |