A Simple but Effective Combination of pH Indicators for Plant Tissue Culture
The use of pH indicators provides a simple, semi-quantitative visual method for quickly assessing pH changes in tissue culture media; however, pH indicators are rarely used in routine plant tissue culture media. In this study, chlorophenol red, bromocresol purple, and bromocresol green were tested t...
| Main Authors: | , , |
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| Format: | Journal Article |
| Language: | English |
| Published: |
MDPI
2023
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| Subjects: | |
| Online Access: | http://purl.org/au-research/grants/arc/LP210200907 http://hdl.handle.net/20.500.11937/96292 |
| _version_ | 1848766129500061696 |
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| author | Funnekotter, Bryn Mancera, Ricardo Bunn, E. |
| author_facet | Funnekotter, Bryn Mancera, Ricardo Bunn, E. |
| author_sort | Funnekotter, Bryn |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | The use of pH indicators provides a simple, semi-quantitative visual method for quickly assessing pH changes in tissue culture media; however, pH indicators are rarely used in routine plant tissue culture media. In this study, chlorophenol red, bromocresol purple, and bromocresol green were tested to assess their functionality in the growth medium for plant shoot cultures. In addition, a combination of bromocresol green and bromocresol purple was tested to determine if they would widen the observable colour change to better assess pH changes in the medium. Varying the ratio of bromocresol green to bromocresol purple alters the pH at which the colour changes from blue to green to yellow, with a 1:3 ratio providing a useful pH range of 5–6.5, while a 1:1 ratio provides a useful pH range of 4.5–6. All the pH indicators showed no toxic side effects for the plant species tested in this study and were able to be autoclaved to ensure media sterility. The addition of these pH indicators to quickly assess media pH in large tissue culture collections can aid in routine maintenance. These pH indicators can be used as a ‘traffic light’ system, with blue indicating a high pH, green a normal pH, and yellow a low pH in the media. |
| first_indexed | 2025-11-14T11:46:14Z |
| format | Journal Article |
| id | curtin-20.500.11937-96292 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T11:46:14Z |
| publishDate | 2023 |
| publisher | MDPI |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-962922024-11-19T00:50:40Z A Simple but Effective Combination of pH Indicators for Plant Tissue Culture Funnekotter, Bryn Mancera, Ricardo Bunn, E. Science & Technology Life Sciences & Biomedicine Plant Sciences bromocresol green bromocresol purple chlorophenol red cell culture in vitro culture GROWTH PROTOPLASTS NICOTIANA SELECTION bromocresol green bromocresol purple cell culture chlorophenol red in vitro culture The use of pH indicators provides a simple, semi-quantitative visual method for quickly assessing pH changes in tissue culture media; however, pH indicators are rarely used in routine plant tissue culture media. In this study, chlorophenol red, bromocresol purple, and bromocresol green were tested to assess their functionality in the growth medium for plant shoot cultures. In addition, a combination of bromocresol green and bromocresol purple was tested to determine if they would widen the observable colour change to better assess pH changes in the medium. Varying the ratio of bromocresol green to bromocresol purple alters the pH at which the colour changes from blue to green to yellow, with a 1:3 ratio providing a useful pH range of 5–6.5, while a 1:1 ratio provides a useful pH range of 4.5–6. All the pH indicators showed no toxic side effects for the plant species tested in this study and were able to be autoclaved to ensure media sterility. The addition of these pH indicators to quickly assess media pH in large tissue culture collections can aid in routine maintenance. These pH indicators can be used as a ‘traffic light’ system, with blue indicating a high pH, green a normal pH, and yellow a low pH in the media. 2023 Journal Article http://hdl.handle.net/20.500.11937/96292 10.3390/plants12040740 English http://purl.org/au-research/grants/arc/LP210200907 http://creativecommons.org/licenses/by/4.0/ MDPI fulltext |
| spellingShingle | Science & Technology Life Sciences & Biomedicine Plant Sciences bromocresol green bromocresol purple chlorophenol red cell culture in vitro culture GROWTH PROTOPLASTS NICOTIANA SELECTION bromocresol green bromocresol purple cell culture chlorophenol red in vitro culture Funnekotter, Bryn Mancera, Ricardo Bunn, E. A Simple but Effective Combination of pH Indicators for Plant Tissue Culture |
| title | A Simple but Effective Combination of pH Indicators for Plant Tissue Culture |
| title_full | A Simple but Effective Combination of pH Indicators for Plant Tissue Culture |
| title_fullStr | A Simple but Effective Combination of pH Indicators for Plant Tissue Culture |
| title_full_unstemmed | A Simple but Effective Combination of pH Indicators for Plant Tissue Culture |
| title_short | A Simple but Effective Combination of pH Indicators for Plant Tissue Culture |
| title_sort | simple but effective combination of ph indicators for plant tissue culture |
| topic | Science & Technology Life Sciences & Biomedicine Plant Sciences bromocresol green bromocresol purple chlorophenol red cell culture in vitro culture GROWTH PROTOPLASTS NICOTIANA SELECTION bromocresol green bromocresol purple cell culture chlorophenol red in vitro culture |
| url | http://purl.org/au-research/grants/arc/LP210200907 http://hdl.handle.net/20.500.11937/96292 |