Rhizopine biosensors for plant-dependent control of bacterial gene expression
Engineering signalling between plants and microbes could be exploited to establish host-specificity between plant-growth-promoting bacteria and target crops in the environment. We previously engineered rhizopine-signalling circuitry facilitating exclusive signalling between rhizopine-producing (RhiP...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Journal Article |
| Language: | English |
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WILEY
2023
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| Online Access: | http://purl.org/au-research/grants/arc/FT170100235 http://hdl.handle.net/20.500.11937/96176 |
| _version_ | 1848766107785101312 |
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| author | Haskett, T.L. Geddes, B.A. Paramasivan, P. Green, P. Chitnavis, S. Mendes, M.D. Jorrín, B. Knights, H.E. Bastholm, Tahlia Ramsay, Joshua Oldroyd, G.E.D. Poole, P.S. |
| author_facet | Haskett, T.L. Geddes, B.A. Paramasivan, P. Green, P. Chitnavis, S. Mendes, M.D. Jorrín, B. Knights, H.E. Bastholm, Tahlia Ramsay, Joshua Oldroyd, G.E.D. Poole, P.S. |
| author_sort | Haskett, T.L. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | Engineering signalling between plants and microbes could be exploited to establish host-specificity between plant-growth-promoting bacteria and target crops in the environment. We previously engineered rhizopine-signalling circuitry facilitating exclusive signalling between rhizopine-producing (RhiP) plants and model bacterial strains. Here, we conduct an in-depth analysis of rhizopine-inducible expression in bacteria. We characterize two rhizopine-inducible promoters and explore the bacterial host-range of rhizopine biosensor plasmids. By tuning the expression of rhizopine uptake genes, we also construct a new biosensor plasmid pSIR05 that has minimal impact on host cell growth in vitro and exhibits markedly improved stability of expression in situ on RhiP barley roots compared to the previously described biosensor plasmid pSIR02. We demonstrate that a sub-population of Azorhizobium caulinodans cells carrying pSIR05 can sense rhizopine and activate gene expression when colonizing RhiP barley roots. However, these bacteria were mildly defective for colonization of RhiP barley roots compared to the wild-type parent strain. This work provides advancement towards establishing more robust plant-dependent control of bacterial gene expression and highlights the key challenges remaining to achieve this goal. |
| first_indexed | 2025-11-14T11:45:53Z |
| format | Journal Article |
| id | curtin-20.500.11937-96176 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T11:45:53Z |
| publishDate | 2023 |
| publisher | WILEY |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-961762024-11-07T23:38:58Z Rhizopine biosensors for plant-dependent control of bacterial gene expression Haskett, T.L. Geddes, B.A. Paramasivan, P. Green, P. Chitnavis, S. Mendes, M.D. Jorrín, B. Knights, H.E. Bastholm, Tahlia Ramsay, Joshua Oldroyd, G.E.D. Poole, P.S. Science & Technology Life Sciences & Biomedicine Microbiology RHIZOBIUM-MELILOTI NITROGEN-FIXATION ESCHERICHIA-COLI IN-VIVO RHIZOSPHERE CATABOLISM TRANSCRIPTION REGULATOR PROTEIN LEGUMINOSARUM Bacteria Genes, Bacterial Biosensing Techniques Gene Expression Bacteria Biosensing Techniques Gene Expression Genes, Bacterial Engineering signalling between plants and microbes could be exploited to establish host-specificity between plant-growth-promoting bacteria and target crops in the environment. We previously engineered rhizopine-signalling circuitry facilitating exclusive signalling between rhizopine-producing (RhiP) plants and model bacterial strains. Here, we conduct an in-depth analysis of rhizopine-inducible expression in bacteria. We characterize two rhizopine-inducible promoters and explore the bacterial host-range of rhizopine biosensor plasmids. By tuning the expression of rhizopine uptake genes, we also construct a new biosensor plasmid pSIR05 that has minimal impact on host cell growth in vitro and exhibits markedly improved stability of expression in situ on RhiP barley roots compared to the previously described biosensor plasmid pSIR02. We demonstrate that a sub-population of Azorhizobium caulinodans cells carrying pSIR05 can sense rhizopine and activate gene expression when colonizing RhiP barley roots. However, these bacteria were mildly defective for colonization of RhiP barley roots compared to the wild-type parent strain. This work provides advancement towards establishing more robust plant-dependent control of bacterial gene expression and highlights the key challenges remaining to achieve this goal. 2023 Journal Article http://hdl.handle.net/20.500.11937/96176 10.1111/1462-2920.16288 English http://purl.org/au-research/grants/arc/FT170100235 http://creativecommons.org/licenses/by/4.0/ WILEY fulltext |
| spellingShingle | Science & Technology Life Sciences & Biomedicine Microbiology RHIZOBIUM-MELILOTI NITROGEN-FIXATION ESCHERICHIA-COLI IN-VIVO RHIZOSPHERE CATABOLISM TRANSCRIPTION REGULATOR PROTEIN LEGUMINOSARUM Bacteria Genes, Bacterial Biosensing Techniques Gene Expression Bacteria Biosensing Techniques Gene Expression Genes, Bacterial Haskett, T.L. Geddes, B.A. Paramasivan, P. Green, P. Chitnavis, S. Mendes, M.D. Jorrín, B. Knights, H.E. Bastholm, Tahlia Ramsay, Joshua Oldroyd, G.E.D. Poole, P.S. Rhizopine biosensors for plant-dependent control of bacterial gene expression |
| title | Rhizopine biosensors for plant-dependent control of bacterial gene expression |
| title_full | Rhizopine biosensors for plant-dependent control of bacterial gene expression |
| title_fullStr | Rhizopine biosensors for plant-dependent control of bacterial gene expression |
| title_full_unstemmed | Rhizopine biosensors for plant-dependent control of bacterial gene expression |
| title_short | Rhizopine biosensors for plant-dependent control of bacterial gene expression |
| title_sort | rhizopine biosensors for plant-dependent control of bacterial gene expression |
| topic | Science & Technology Life Sciences & Biomedicine Microbiology RHIZOBIUM-MELILOTI NITROGEN-FIXATION ESCHERICHIA-COLI IN-VIVO RHIZOSPHERE CATABOLISM TRANSCRIPTION REGULATOR PROTEIN LEGUMINOSARUM Bacteria Genes, Bacterial Biosensing Techniques Gene Expression Bacteria Biosensing Techniques Gene Expression Genes, Bacterial |
| url | http://purl.org/au-research/grants/arc/FT170100235 http://hdl.handle.net/20.500.11937/96176 |