Genomic characterization of a unique Panton–Valentine leucocidin-positive community-associated methicillin-resistant Staphylococcus aureus lineage increasingly impacting on Australian indigenous communities
In 2010 a single isolate of a trimethoprim-resistant multilocus sequence type 5, Panton–Valentine leucocidin-positive, community-associated methicillin-resistant Staphylococcus aureus (PVL-positive ST5 CA-MRSA), colloquially named WA121, was identified in northern Western Australia (WA). WA121 now a...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Journal Article |
| Language: | English |
| Published: |
2023
|
| Subjects: | |
| Online Access: | http://purl.org/au-research/grants/arc/FT170100235 http://hdl.handle.net/20.500.11937/96175 |
| _version_ | 1848766107475771392 |
|---|---|
| author | Ramsay, Joshua Parahitiyawa, Nipuna Mowlaboccus, S. Mullally, C.A. Yee, N.W.T. Shoby, P. Colombi, Elena Tan, H.L. Pearson, J.C. Coombs, G.W. |
| author_facet | Ramsay, Joshua Parahitiyawa, Nipuna Mowlaboccus, S. Mullally, C.A. Yee, N.W.T. Shoby, P. Colombi, Elena Tan, H.L. Pearson, J.C. Coombs, G.W. |
| author_sort | Ramsay, Joshua |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | In 2010 a single isolate of a trimethoprim-resistant multilocus sequence type 5, Panton–Valentine leucocidin-positive, community-associated methicillin-resistant Staphylococcus aureus (PVL-positive ST5 CA-MRSA), colloquially named WA121, was identified in northern Western Australia (WA). WA121 now accounts for ~14 % of all WA MRSA infections. To gain an understanding of the genetic composition and phylogenomic structure of WA121 isolates we sequenced the genomes of 155 WA121 isolates collected 2010–2021 and present a detailed genomic description. WA121 was revealed to be a single clonally expanding lineage clearly distinct from sequenced ST5 strains reported outside Australia. WA121 strains were typified by the presence of the distinct PVL phage φSa2wa-st5, the recently described methicillin resistance element SCCmecIVo carrying the trimethoprim resistance (dfrG) transposon Tn4791, the novel β-lactamase transposon Tn7702 and the epidermal cell differentiation inhibitor (EDIN-A) plasmid p2010-15611-2. We present evidence that SCCmecIVo together with Tn4791 has horizontally transferred to Staphylococcus argenteus and evidence of intragenomic movement of both Tn4791 and Tn7702. We experimentally demonstrate that p2010-15611-2 is capable of horizontal transfer by conjugative mobilization from one of several WA121 isolates also har-bouring a pWBG749-like conjugative plasmid. In summary, WA121 is a distinct and clonally expanding Australian PVL-positive CA-MRSA lineage that is increasingly responsible for infections in indigenous communities in northern and western Australia. WA121 harbours a unique complement of mobile genetic elements and is capable of transferring antimicrobial resistance and virulence determinants to other staphylococci. |
| first_indexed | 2025-11-14T11:45:53Z |
| format | Journal Article |
| id | curtin-20.500.11937-96175 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| language | eng |
| last_indexed | 2025-11-14T11:45:53Z |
| publishDate | 2023 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-961752024-11-07T05:08:02Z Genomic characterization of a unique Panton–Valentine leucocidin-positive community-associated methicillin-resistant Staphylococcus aureus lineage increasingly impacting on Australian indigenous communities Ramsay, Joshua Parahitiyawa, Nipuna Mowlaboccus, S. Mullally, C.A. Yee, N.W.T. Shoby, P. Colombi, Elena Tan, H.L. Pearson, J.C. Coombs, G.W. PVL-positive Panton–Valentine leucocidin ST5 CA-MRSA Tn4791 Tn916 Tn7702 Tn553 conjugative mobilization dfrG SXT Methicillin-Resistant Staphylococcus aureus Australia Leukocidins Genomics Western Australia Leukocidins Genomics Australia Western Australia Methicillin-Resistant Staphylococcus aureus In 2010 a single isolate of a trimethoprim-resistant multilocus sequence type 5, Panton–Valentine leucocidin-positive, community-associated methicillin-resistant Staphylococcus aureus (PVL-positive ST5 CA-MRSA), colloquially named WA121, was identified in northern Western Australia (WA). WA121 now accounts for ~14 % of all WA MRSA infections. To gain an understanding of the genetic composition and phylogenomic structure of WA121 isolates we sequenced the genomes of 155 WA121 isolates collected 2010–2021 and present a detailed genomic description. WA121 was revealed to be a single clonally expanding lineage clearly distinct from sequenced ST5 strains reported outside Australia. WA121 strains were typified by the presence of the distinct PVL phage φSa2wa-st5, the recently described methicillin resistance element SCCmecIVo carrying the trimethoprim resistance (dfrG) transposon Tn4791, the novel β-lactamase transposon Tn7702 and the epidermal cell differentiation inhibitor (EDIN-A) plasmid p2010-15611-2. We present evidence that SCCmecIVo together with Tn4791 has horizontally transferred to Staphylococcus argenteus and evidence of intragenomic movement of both Tn4791 and Tn7702. We experimentally demonstrate that p2010-15611-2 is capable of horizontal transfer by conjugative mobilization from one of several WA121 isolates also har-bouring a pWBG749-like conjugative plasmid. In summary, WA121 is a distinct and clonally expanding Australian PVL-positive CA-MRSA lineage that is increasingly responsible for infections in indigenous communities in northern and western Australia. WA121 harbours a unique complement of mobile genetic elements and is capable of transferring antimicrobial resistance and virulence determinants to other staphylococci. 2023 Journal Article http://hdl.handle.net/20.500.11937/96175 10.1099/mgen.0.001172 eng http://purl.org/au-research/grants/arc/FT170100235 http://creativecommons.org/licenses/by/4.0/deed.ast fulltext |
| spellingShingle | PVL-positive Panton–Valentine leucocidin ST5 CA-MRSA Tn4791 Tn916 Tn7702 Tn553 conjugative mobilization dfrG SXT Methicillin-Resistant Staphylococcus aureus Australia Leukocidins Genomics Western Australia Leukocidins Genomics Australia Western Australia Methicillin-Resistant Staphylococcus aureus Ramsay, Joshua Parahitiyawa, Nipuna Mowlaboccus, S. Mullally, C.A. Yee, N.W.T. Shoby, P. Colombi, Elena Tan, H.L. Pearson, J.C. Coombs, G.W. Genomic characterization of a unique Panton–Valentine leucocidin-positive community-associated methicillin-resistant Staphylococcus aureus lineage increasingly impacting on Australian indigenous communities |
| title | Genomic characterization of a unique Panton–Valentine leucocidin-positive community-associated methicillin-resistant Staphylococcus aureus lineage increasingly impacting on Australian indigenous communities |
| title_full | Genomic characterization of a unique Panton–Valentine leucocidin-positive community-associated methicillin-resistant Staphylococcus aureus lineage increasingly impacting on Australian indigenous communities |
| title_fullStr | Genomic characterization of a unique Panton–Valentine leucocidin-positive community-associated methicillin-resistant Staphylococcus aureus lineage increasingly impacting on Australian indigenous communities |
| title_full_unstemmed | Genomic characterization of a unique Panton–Valentine leucocidin-positive community-associated methicillin-resistant Staphylococcus aureus lineage increasingly impacting on Australian indigenous communities |
| title_short | Genomic characterization of a unique Panton–Valentine leucocidin-positive community-associated methicillin-resistant Staphylococcus aureus lineage increasingly impacting on Australian indigenous communities |
| title_sort | genomic characterization of a unique panton–valentine leucocidin-positive community-associated methicillin-resistant staphylococcus aureus lineage increasingly impacting on australian indigenous communities |
| topic | PVL-positive Panton–Valentine leucocidin ST5 CA-MRSA Tn4791 Tn916 Tn7702 Tn553 conjugative mobilization dfrG SXT Methicillin-Resistant Staphylococcus aureus Australia Leukocidins Genomics Western Australia Leukocidins Genomics Australia Western Australia Methicillin-Resistant Staphylococcus aureus |
| url | http://purl.org/au-research/grants/arc/FT170100235 http://hdl.handle.net/20.500.11937/96175 |