The Velvet transcription factor PnVeA regulates necrotrophic effectors and secondary metabolism in the wheat pathogen Parastagonospora nodorum
The fungus Parastagonospora nodorum causes septoria nodorum blotch on wheat. The role of the fungal Velvet-family transcription factor VeA in P. nodorum development and virulence was investigated here. Deletion of the P. nodorum VeA ortholog, PnVeA, resulted in growth abnormalities including pigment...
| Main Authors: | , , , , , , , , , |
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| Format: | Journal Article |
| Language: | English |
| Published: |
2024
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| Subjects: | |
| Online Access: | http://hdl.handle.net/20.500.11937/96023 |
| _version_ | 1848766075330625536 |
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| author | Morikawa, Shota Verdonk, Callum John, Evan Lenzo, Leon Sbaraini, Nicolau Turo, Chala Li, Hang Jiang, David Chooi, Y.H. Tan, Kar-Chun |
| author_facet | Morikawa, Shota Verdonk, Callum John, Evan Lenzo, Leon Sbaraini, Nicolau Turo, Chala Li, Hang Jiang, David Chooi, Y.H. Tan, Kar-Chun |
| author_sort | Morikawa, Shota |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | The fungus Parastagonospora nodorum causes septoria nodorum blotch on wheat. The role of the fungal Velvet-family transcription factor VeA in P. nodorum development and virulence was investigated here. Deletion of the P. nodorum VeA ortholog, PnVeA, resulted in growth abnormalities including pigmentation, abolished asexual sporulation and highly reduced virulence on wheat. Comparative RNA-Seq and RT-PCR analyses revealed that the deletion of PnVeA also decoupled the expression of major necrotrophic effector genes. In addition, the deletion of PnVeA resulted in an up-regulation of four predicted secondary metabolite (SM) gene clusters. Using liquid-chromatography mass-spectrometry, it was observed that one of the SM gene clusters led to an accumulation of the mycotoxin alternariol. PnVeA is essential for asexual sporulation, full virulence, secondary metabolism and necrotrophic effector regulation. |
| first_indexed | 2025-11-14T11:45:23Z |
| format | Journal Article |
| id | curtin-20.500.11937-96023 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| language | eng |
| last_indexed | 2025-11-14T11:45:23Z |
| publishDate | 2024 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-960232024-11-13T07:05:09Z The Velvet transcription factor PnVeA regulates necrotrophic effectors and secondary metabolism in the wheat pathogen Parastagonospora nodorum Morikawa, Shota Verdonk, Callum John, Evan Lenzo, Leon Sbaraini, Nicolau Turo, Chala Li, Hang Jiang, David Chooi, Y.H. Tan, Kar-Chun Parastagonospora nodorum Necrotrophic effector Secondary metabolite Velvet A Wheat Ascomycota Fungal Proteins Gene Expression Regulation, Fungal Lactones Multigene Family Mycotoxins Plant Diseases Secondary Metabolism Spores, Fungal Transcription Factors Triticum Virulence Ascomycota Spores, Fungal Triticum Lactones Fungal Proteins Transcription Factors Mycotoxins Virulence Plant Diseases Gene Expression Regulation, Fungal Multigene Family Secondary Metabolism The fungus Parastagonospora nodorum causes septoria nodorum blotch on wheat. The role of the fungal Velvet-family transcription factor VeA in P. nodorum development and virulence was investigated here. Deletion of the P. nodorum VeA ortholog, PnVeA, resulted in growth abnormalities including pigmentation, abolished asexual sporulation and highly reduced virulence on wheat. Comparative RNA-Seq and RT-PCR analyses revealed that the deletion of PnVeA also decoupled the expression of major necrotrophic effector genes. In addition, the deletion of PnVeA resulted in an up-regulation of four predicted secondary metabolite (SM) gene clusters. Using liquid-chromatography mass-spectrometry, it was observed that one of the SM gene clusters led to an accumulation of the mycotoxin alternariol. PnVeA is essential for asexual sporulation, full virulence, secondary metabolism and necrotrophic effector regulation. 2024 Journal Article http://hdl.handle.net/20.500.11937/96023 10.1186/s12866-024-03454-7 eng http://creativecommons.org/licenses/by-nc-nd/4.0/ fulltext |
| spellingShingle | Parastagonospora nodorum Necrotrophic effector Secondary metabolite Velvet A Wheat Ascomycota Fungal Proteins Gene Expression Regulation, Fungal Lactones Multigene Family Mycotoxins Plant Diseases Secondary Metabolism Spores, Fungal Transcription Factors Triticum Virulence Ascomycota Spores, Fungal Triticum Lactones Fungal Proteins Transcription Factors Mycotoxins Virulence Plant Diseases Gene Expression Regulation, Fungal Multigene Family Secondary Metabolism Morikawa, Shota Verdonk, Callum John, Evan Lenzo, Leon Sbaraini, Nicolau Turo, Chala Li, Hang Jiang, David Chooi, Y.H. Tan, Kar-Chun The Velvet transcription factor PnVeA regulates necrotrophic effectors and secondary metabolism in the wheat pathogen Parastagonospora nodorum |
| title | The Velvet transcription factor PnVeA regulates necrotrophic effectors and secondary metabolism in the wheat pathogen Parastagonospora nodorum |
| title_full | The Velvet transcription factor PnVeA regulates necrotrophic effectors and secondary metabolism in the wheat pathogen Parastagonospora nodorum |
| title_fullStr | The Velvet transcription factor PnVeA regulates necrotrophic effectors and secondary metabolism in the wheat pathogen Parastagonospora nodorum |
| title_full_unstemmed | The Velvet transcription factor PnVeA regulates necrotrophic effectors and secondary metabolism in the wheat pathogen Parastagonospora nodorum |
| title_short | The Velvet transcription factor PnVeA regulates necrotrophic effectors and secondary metabolism in the wheat pathogen Parastagonospora nodorum |
| title_sort | velvet transcription factor pnvea regulates necrotrophic effectors and secondary metabolism in the wheat pathogen parastagonospora nodorum |
| topic | Parastagonospora nodorum Necrotrophic effector Secondary metabolite Velvet A Wheat Ascomycota Fungal Proteins Gene Expression Regulation, Fungal Lactones Multigene Family Mycotoxins Plant Diseases Secondary Metabolism Spores, Fungal Transcription Factors Triticum Virulence Ascomycota Spores, Fungal Triticum Lactones Fungal Proteins Transcription Factors Mycotoxins Virulence Plant Diseases Gene Expression Regulation, Fungal Multigene Family Secondary Metabolism |
| url | http://hdl.handle.net/20.500.11937/96023 |