The respiratory health effects of acute in vivo diesel and biodiesel exhaust in a mouse model
Background: Biodiesel, a renewable diesel fuel that can be created from almost any natural fat or oil, is promoted as a greener and healthier alternative to commercial mineral diesel without the supporting experimental data to back these claims. The aim of this research was to assess the health effe...
| Main Authors: | , , , , , |
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| Format: | Journal Article |
| Language: | English |
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2024
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| Online Access: | http://purl.org/au-research/grants/arc/DP170104346 http://hdl.handle.net/20.500.11937/96016 |
| _version_ | 1848766074338672640 |
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| author | Landwehr, Katherine Mead-Hunter, Ryan O'Leary, R.A. Kicic, Anthony Mullins, Ben Larcombe, Alexander |
| author_facet | Landwehr, Katherine Mead-Hunter, Ryan O'Leary, R.A. Kicic, Anthony Mullins, Ben Larcombe, Alexander |
| author_sort | Landwehr, Katherine |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | Background: Biodiesel, a renewable diesel fuel that can be created from almost any natural fat or oil, is promoted as a greener and healthier alternative to commercial mineral diesel without the supporting experimental data to back these claims. The aim of this research was to assess the health effects of acute exposure to two types of biodiesel exhaust, or mineral diesel exhaust or air as a control in mice. Male BALB/c mice were exposed for 2-hrs to diluted exhaust obtained from a diesel engine running on mineral diesel, Tallow biodiesel or Canola biodiesel. A room air exposure group was used as a control. Twenty-four hours after exposure, a variety of respiratory related end point measurements were assessed, including lung function, responsiveness to methacholine and airway and systemic immune responses. Results: Tallow biodiesel exhaust exposure resulted in the greatest number of significant effects compared to Air controls, including increased airway hyperresponsiveness (178.1 ± 31.3% increase from saline for Tallow biodiesel exhaust exposed mice compared to 155.8 ± 19.1 for Air control), increased airway inflammation (63463 ± 13497 cells/mL in the bronchoalveolar lavage of Tallow biodiesel exhaust exposed mice compared to 40561 ± 11800 for Air exposed controls) and indications of immune dysregulation. In contrast, exposure to Canola biodiesel exhaust resulted in fewer significant effects compared to Air controls with a slight increase in airway resistance at functional residual capacity and indications of immune dysregulation. Exposure to mineral diesel exhaust resulted in significant effects between that of the two biodiesels with increased airway hyperresponsiveness and indications of immune dysregulation. Conclusion: These data show that a single, brief exposure to biodiesel exhaust can result in negative health impacts in a mouse model, and that the biological effects of exposure change depending on the feedstock used to make the biodiesel. |
| first_indexed | 2025-11-14T11:45:22Z |
| format | Journal Article |
| id | curtin-20.500.11937-96016 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| language | eng |
| last_indexed | 2025-11-14T11:45:22Z |
| publishDate | 2024 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-960162024-11-07T05:00:50Z The respiratory health effects of acute in vivo diesel and biodiesel exhaust in a mouse model Landwehr, Katherine Mead-Hunter, Ryan O'Leary, R.A. Kicic, Anthony Mullins, Ben Larcombe, Alexander Biodiesel health effects Exhaust toxicology Lung function Mouse model Animals Vehicle Emissions Biofuels Mice Mice, Inbred BALB C Male Gasoline Air Pollutants Lung Bronchoalveolar Lavage Fluid Inhalation Exposure Lung Bronchoalveolar Lavage Fluid Animals Mice, Inbred BALB C Mice Air Pollutants Gasoline Vehicle Emissions Inhalation Exposure Male Biofuels Background: Biodiesel, a renewable diesel fuel that can be created from almost any natural fat or oil, is promoted as a greener and healthier alternative to commercial mineral diesel without the supporting experimental data to back these claims. The aim of this research was to assess the health effects of acute exposure to two types of biodiesel exhaust, or mineral diesel exhaust or air as a control in mice. Male BALB/c mice were exposed for 2-hrs to diluted exhaust obtained from a diesel engine running on mineral diesel, Tallow biodiesel or Canola biodiesel. A room air exposure group was used as a control. Twenty-four hours after exposure, a variety of respiratory related end point measurements were assessed, including lung function, responsiveness to methacholine and airway and systemic immune responses. Results: Tallow biodiesel exhaust exposure resulted in the greatest number of significant effects compared to Air controls, including increased airway hyperresponsiveness (178.1 ± 31.3% increase from saline for Tallow biodiesel exhaust exposed mice compared to 155.8 ± 19.1 for Air control), increased airway inflammation (63463 ± 13497 cells/mL in the bronchoalveolar lavage of Tallow biodiesel exhaust exposed mice compared to 40561 ± 11800 for Air exposed controls) and indications of immune dysregulation. In contrast, exposure to Canola biodiesel exhaust resulted in fewer significant effects compared to Air controls with a slight increase in airway resistance at functional residual capacity and indications of immune dysregulation. Exposure to mineral diesel exhaust resulted in significant effects between that of the two biodiesels with increased airway hyperresponsiveness and indications of immune dysregulation. Conclusion: These data show that a single, brief exposure to biodiesel exhaust can result in negative health impacts in a mouse model, and that the biological effects of exposure change depending on the feedstock used to make the biodiesel. 2024 Journal Article http://hdl.handle.net/20.500.11937/96016 10.1016/j.chemosphere.2024.142621 eng http://purl.org/au-research/grants/arc/DP170104346 https://creativecommons.org/licenses/by/4.0/ fulltext |
| spellingShingle | Biodiesel health effects Exhaust toxicology Lung function Mouse model Animals Vehicle Emissions Biofuels Mice Mice, Inbred BALB C Male Gasoline Air Pollutants Lung Bronchoalveolar Lavage Fluid Inhalation Exposure Lung Bronchoalveolar Lavage Fluid Animals Mice, Inbred BALB C Mice Air Pollutants Gasoline Vehicle Emissions Inhalation Exposure Male Biofuels Landwehr, Katherine Mead-Hunter, Ryan O'Leary, R.A. Kicic, Anthony Mullins, Ben Larcombe, Alexander The respiratory health effects of acute in vivo diesel and biodiesel exhaust in a mouse model |
| title | The respiratory health effects of acute in vivo diesel and biodiesel exhaust in a mouse model |
| title_full | The respiratory health effects of acute in vivo diesel and biodiesel exhaust in a mouse model |
| title_fullStr | The respiratory health effects of acute in vivo diesel and biodiesel exhaust in a mouse model |
| title_full_unstemmed | The respiratory health effects of acute in vivo diesel and biodiesel exhaust in a mouse model |
| title_short | The respiratory health effects of acute in vivo diesel and biodiesel exhaust in a mouse model |
| title_sort | respiratory health effects of acute in vivo diesel and biodiesel exhaust in a mouse model |
| topic | Biodiesel health effects Exhaust toxicology Lung function Mouse model Animals Vehicle Emissions Biofuels Mice Mice, Inbred BALB C Male Gasoline Air Pollutants Lung Bronchoalveolar Lavage Fluid Inhalation Exposure Lung Bronchoalveolar Lavage Fluid Animals Mice, Inbred BALB C Mice Air Pollutants Gasoline Vehicle Emissions Inhalation Exposure Male Biofuels |
| url | http://purl.org/au-research/grants/arc/DP170104346 http://hdl.handle.net/20.500.11937/96016 |