Apoplastic effector candidates of a foliar forest pathogen trigger cell death in host and non-host plants
Forests are under threat from pests, pathogens, and changing climate. A major forest pathogen worldwide is the hemibiotroph Dothistroma septosporum, which causes dothistroma needle blight (DNB) of pines. While D. septosporum uses effector proteins to facilitate host infection, it is currently unclea...
| Main Authors: | , , , , , , , , |
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| Format: | Journal Article |
| Language: | English |
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NATURE PORTFOLIO
2021
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| Subjects: | |
| Online Access: | http://hdl.handle.net/20.500.11937/95662 |
| _version_ | 1848766041411289088 |
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| author | Hunziker, Lukas Tarallo, M. Gough, K. Guo, M. Hargreaves, C. Loo, T.S. McDougal, R.L. Mesarich, C.H. Bradshaw, R.E. |
| author_facet | Hunziker, Lukas Tarallo, M. Gough, K. Guo, M. Hargreaves, C. Loo, T.S. McDougal, R.L. Mesarich, C.H. Bradshaw, R.E. |
| author_sort | Hunziker, Lukas |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | Forests are under threat from pests, pathogens, and changing climate. A major forest pathogen worldwide is the hemibiotroph Dothistroma septosporum, which causes dothistroma needle blight (DNB) of pines. While D. septosporum uses effector proteins to facilitate host infection, it is currently unclear whether any of these effectors are recognised by immune receptors to activate the host immune system. Such information is needed to identify and select disease resistance against D. septosporum in pines. We predicted and investigated apoplastic D. septosporum candidate effectors (DsCEs) using bioinformatics and plant-based experiments. We discovered DsCEs that trigger cell death in the angiosperm Nicotiana spp., indicative of a hypersensitive defence response and suggesting their recognition by immune receptors in non-host plants. In a first for foliar forest pathogens, we developed a novel protein infiltration method to show that tissue-cultured pine shoots can respond with a cell death response to a DsCE, as well as to a reference cell death-inducing protein. The conservation of responses across plant taxa suggests that knowledge of pathogen–angiosperm interactions may also be relevant to pathogen–gymnosperm interactions. These results contribute to our understanding of forest pathogens and may ultimately provide clues to disease immunity in both commercial and natural forests. |
| first_indexed | 2025-11-14T11:44:50Z |
| format | Journal Article |
| id | curtin-20.500.11937-95662 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T11:44:50Z |
| publishDate | 2021 |
| publisher | NATURE PORTFOLIO |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-956622024-10-10T04:02:44Z Apoplastic effector candidates of a foliar forest pathogen trigger cell death in host and non-host plants Hunziker, Lukas Tarallo, M. Gough, K. Guo, M. Hargreaves, C. Loo, T.S. McDougal, R.L. Mesarich, C.H. Bradshaw, R.E. Science & Technology Multidisciplinary Sciences Science & Technology - Other Topics DOTHISTROMA NEEDLE BLIGHT CLADOSPORIUM-FULVUM FUNGAL EFFECTORS VIRULENCE FACTOR RICH PROTEIN RESISTANCE RECOGNITION GENE ELICITOR IMMUNITY Ascomycota Cell Death Disease Resistance Host-Pathogen Interactions Pinus Plant Diseases Nicotiana Ascomycota Pinus Cell Death Plant Diseases Host-Pathogen Interactions Disease Resistance Nicotiana Forests are under threat from pests, pathogens, and changing climate. A major forest pathogen worldwide is the hemibiotroph Dothistroma septosporum, which causes dothistroma needle blight (DNB) of pines. While D. septosporum uses effector proteins to facilitate host infection, it is currently unclear whether any of these effectors are recognised by immune receptors to activate the host immune system. Such information is needed to identify and select disease resistance against D. septosporum in pines. We predicted and investigated apoplastic D. septosporum candidate effectors (DsCEs) using bioinformatics and plant-based experiments. We discovered DsCEs that trigger cell death in the angiosperm Nicotiana spp., indicative of a hypersensitive defence response and suggesting their recognition by immune receptors in non-host plants. In a first for foliar forest pathogens, we developed a novel protein infiltration method to show that tissue-cultured pine shoots can respond with a cell death response to a DsCE, as well as to a reference cell death-inducing protein. The conservation of responses across plant taxa suggests that knowledge of pathogen–angiosperm interactions may also be relevant to pathogen–gymnosperm interactions. These results contribute to our understanding of forest pathogens and may ultimately provide clues to disease immunity in both commercial and natural forests. 2021 Journal Article http://hdl.handle.net/20.500.11937/95662 10.1038/s41598-021-99415-5 English http://creativecommons.org/licenses/by/4.0/ NATURE PORTFOLIO fulltext |
| spellingShingle | Science & Technology Multidisciplinary Sciences Science & Technology - Other Topics DOTHISTROMA NEEDLE BLIGHT CLADOSPORIUM-FULVUM FUNGAL EFFECTORS VIRULENCE FACTOR RICH PROTEIN RESISTANCE RECOGNITION GENE ELICITOR IMMUNITY Ascomycota Cell Death Disease Resistance Host-Pathogen Interactions Pinus Plant Diseases Nicotiana Ascomycota Pinus Cell Death Plant Diseases Host-Pathogen Interactions Disease Resistance Nicotiana Hunziker, Lukas Tarallo, M. Gough, K. Guo, M. Hargreaves, C. Loo, T.S. McDougal, R.L. Mesarich, C.H. Bradshaw, R.E. Apoplastic effector candidates of a foliar forest pathogen trigger cell death in host and non-host plants |
| title | Apoplastic effector candidates of a foliar forest pathogen trigger cell death in host and non-host plants |
| title_full | Apoplastic effector candidates of a foliar forest pathogen trigger cell death in host and non-host plants |
| title_fullStr | Apoplastic effector candidates of a foliar forest pathogen trigger cell death in host and non-host plants |
| title_full_unstemmed | Apoplastic effector candidates of a foliar forest pathogen trigger cell death in host and non-host plants |
| title_short | Apoplastic effector candidates of a foliar forest pathogen trigger cell death in host and non-host plants |
| title_sort | apoplastic effector candidates of a foliar forest pathogen trigger cell death in host and non-host plants |
| topic | Science & Technology Multidisciplinary Sciences Science & Technology - Other Topics DOTHISTROMA NEEDLE BLIGHT CLADOSPORIUM-FULVUM FUNGAL EFFECTORS VIRULENCE FACTOR RICH PROTEIN RESISTANCE RECOGNITION GENE ELICITOR IMMUNITY Ascomycota Cell Death Disease Resistance Host-Pathogen Interactions Pinus Plant Diseases Nicotiana Ascomycota Pinus Cell Death Plant Diseases Host-Pathogen Interactions Disease Resistance Nicotiana |
| url | http://hdl.handle.net/20.500.11937/95662 |