Insulinotropic effect of Teucrium polium; Identification of potential mechanistic drug targets
Teucrium polium (T. polium), commonly known as golden germander, is a herb that grows in the Mediterranean region and is used by the locals to treat diabetes mellitus. The glucose-lowering effect of T. polium is well established in rodents. This study aims to examine whether the glucose-lowering eff...
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| Format: | Journal Article |
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Net Journals
2024
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| Online Access: | http://hdl.handle.net/20.500.11937/95312 |
| _version_ | 1848765998769897472 |
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| author | Mannan, Adnan Helmerhorst, Erik Carlessi, Rodrigo Caccetta, Rima |
| author_facet | Mannan, Adnan Helmerhorst, Erik Carlessi, Rodrigo Caccetta, Rima |
| author_sort | Mannan, Adnan |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | Teucrium polium (T. polium), commonly known as golden germander, is a herb that grows in the Mediterranean region and is used by the locals to treat diabetes mellitus. The glucose-lowering effect of T. polium is well established in rodents. This study aims to examine whether the glucose-lowering effect is by inducing the release of insulin from pancreatic beta cells and to identify key measurable biomolecules/sites that are modified in the lead-up to insulin release. Insulin secretion was examined in BRIN-BD11 pancreatic beta cells treated with T. polium extract in vitro and key flavonoids were identified using LCMS. The T. polium extract promoted increased insulin secretion (p<0.05), in a dose-dependent manner, and increased glucose uptake (p<0.05) within 30min. This was accompanied by an increase in GLUT2 (p<0.05) and glucokinase (p<0.01) expression. Also, mitochondrial metabolism and glycolytic rates were enhanced (p<0.05), and ATP production increased (p<0.01), which coincided with increased (p<0.01) calcium influx. Rutin and apigenin were detected, but not quercetin, in this extract. T. polium promotes insulin secretion. T. polium does this by GLUT2 and glucokinase expression, increased glucose transport, metabolism and ATP production, ultimately increasing intracellular calcium. |
| first_indexed | 2025-11-14T11:44:10Z |
| format | Journal Article |
| id | curtin-20.500.11937-95312 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T11:44:10Z |
| publishDate | 2024 |
| publisher | Net Journals |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-953122024-08-08T05:35:54Z Insulinotropic effect of Teucrium polium; Identification of potential mechanistic drug targets Mannan, Adnan Helmerhorst, Erik Carlessi, Rodrigo Caccetta, Rima Teucrium polium (T. polium), commonly known as golden germander, is a herb that grows in the Mediterranean region and is used by the locals to treat diabetes mellitus. The glucose-lowering effect of T. polium is well established in rodents. This study aims to examine whether the glucose-lowering effect is by inducing the release of insulin from pancreatic beta cells and to identify key measurable biomolecules/sites that are modified in the lead-up to insulin release. Insulin secretion was examined in BRIN-BD11 pancreatic beta cells treated with T. polium extract in vitro and key flavonoids were identified using LCMS. The T. polium extract promoted increased insulin secretion (p<0.05), in a dose-dependent manner, and increased glucose uptake (p<0.05) within 30min. This was accompanied by an increase in GLUT2 (p<0.05) and glucokinase (p<0.01) expression. Also, mitochondrial metabolism and glycolytic rates were enhanced (p<0.05), and ATP production increased (p<0.01), which coincided with increased (p<0.01) calcium influx. Rutin and apigenin were detected, but not quercetin, in this extract. T. polium promotes insulin secretion. T. polium does this by GLUT2 and glucokinase expression, increased glucose transport, metabolism and ATP production, ultimately increasing intracellular calcium. 2024 Journal Article http://hdl.handle.net/20.500.11937/95312 10.30918/ampr.121.24.012 http://creativecommons.org/licenses/by/4.0/deed.en_US Net Journals fulltext |
| spellingShingle | Mannan, Adnan Helmerhorst, Erik Carlessi, Rodrigo Caccetta, Rima Insulinotropic effect of Teucrium polium; Identification of potential mechanistic drug targets |
| title | Insulinotropic effect of Teucrium polium; Identification of potential mechanistic drug targets |
| title_full | Insulinotropic effect of Teucrium polium; Identification of potential mechanistic drug targets |
| title_fullStr | Insulinotropic effect of Teucrium polium; Identification of potential mechanistic drug targets |
| title_full_unstemmed | Insulinotropic effect of Teucrium polium; Identification of potential mechanistic drug targets |
| title_short | Insulinotropic effect of Teucrium polium; Identification of potential mechanistic drug targets |
| title_sort | insulinotropic effect of teucrium polium; identification of potential mechanistic drug targets |
| url | http://hdl.handle.net/20.500.11937/95312 |