Spider webs capture environmental DNA from terrestrial vertebrates

Environmental DNA holds significant promise as a non-invasive tool for tracking terrestrial biodiversity. However, in non-homogenous terrestrial environments, the continual exploration of new substrates is crucial. Here we test the hypothesis that spider webs can act as passive biofilters, capturi...

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Main Authors: Newton, Joshua, Nevill, Paul, Bateman, Phillip, Campbell, Mathew, Allentoft, Morten
Format: Journal Article
Published: Cell Press 2024
Online Access:http://hdl.handle.net/20.500.11937/94404
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author Newton, Joshua
Nevill, Paul
Bateman, Phillip
Campbell, Mathew
Allentoft, Morten
author_facet Newton, Joshua
Nevill, Paul
Bateman, Phillip
Campbell, Mathew
Allentoft, Morten
author_sort Newton, Joshua
building Curtin Institutional Repository
collection Online Access
description Environmental DNA holds significant promise as a non-invasive tool for tracking terrestrial biodiversity. However, in non-homogenous terrestrial environments, the continual exploration of new substrates is crucial. Here we test the hypothesis that spider webs can act as passive biofilters, capturing eDNA from vertebrates present in the local environment. Using a metabarcoding approach, we detected verte brate eDNA from all analyzed spider webs (N = 49). Spider webs obtained from an Australian woodland locality yielded vertebrate eDNA from 32 different species, including native mammals and birds. In contrast, webs from Perth Zoo, less than 50 km away, yielded eDNA from 61 different vertebrates and produced a highly distinct species composition, largely reflecting exotic species hosted in the zoo. We show that higher animal biomass and proximity to animal enclosures increased eDNA detection probabil ity in the zoo. Our results indicate a tremendous potential for using spider webs as a cost-effective means to monitor terrestrial vertebrates.
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institution Curtin University Malaysia
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last_indexed 2025-11-14T11:42:06Z
publishDate 2024
publisher Cell Press
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spelling curtin-20.500.11937-944042024-04-05T01:27:04Z Spider webs capture environmental DNA from terrestrial vertebrates Newton, Joshua Nevill, Paul Bateman, Phillip Campbell, Mathew Allentoft, Morten Environmental DNA holds significant promise as a non-invasive tool for tracking terrestrial biodiversity. However, in non-homogenous terrestrial environments, the continual exploration of new substrates is crucial. Here we test the hypothesis that spider webs can act as passive biofilters, capturing eDNA from vertebrates present in the local environment. Using a metabarcoding approach, we detected verte brate eDNA from all analyzed spider webs (N = 49). Spider webs obtained from an Australian woodland locality yielded vertebrate eDNA from 32 different species, including native mammals and birds. In contrast, webs from Perth Zoo, less than 50 km away, yielded eDNA from 61 different vertebrates and produced a highly distinct species composition, largely reflecting exotic species hosted in the zoo. We show that higher animal biomass and proximity to animal enclosures increased eDNA detection probabil ity in the zoo. Our results indicate a tremendous potential for using spider webs as a cost-effective means to monitor terrestrial vertebrates. 2024 Journal Article http://hdl.handle.net/20.500.11937/94404 Cell Press fulltext
spellingShingle Newton, Joshua
Nevill, Paul
Bateman, Phillip
Campbell, Mathew
Allentoft, Morten
Spider webs capture environmental DNA from terrestrial vertebrates
title Spider webs capture environmental DNA from terrestrial vertebrates
title_full Spider webs capture environmental DNA from terrestrial vertebrates
title_fullStr Spider webs capture environmental DNA from terrestrial vertebrates
title_full_unstemmed Spider webs capture environmental DNA from terrestrial vertebrates
title_short Spider webs capture environmental DNA from terrestrial vertebrates
title_sort spider webs capture environmental dna from terrestrial vertebrates
url http://hdl.handle.net/20.500.11937/94404