X-ray fluorescence microscopy methods for biological tissues
Synchrotron-based X-ray fluorescence microscopy is a flexible tool for identifying the distribution of trace elements in biological specimens across a broad range of sample sizes. The technique is not particularly limited by sample type and can be performed on ancient fossils, fixed or fresh tissue...
| Main Authors: | , , , , , |
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| Format: | Journal Article |
| Language: | English |
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OXFORD UNIV PRESS
2022
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| Subjects: | |
| Online Access: | http://hdl.handle.net/20.500.11937/92982 |
| _version_ | 1848765682932514816 |
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| author | Pushie, M.J. Sylvain, N.J. Hou, H. Hackett, Mark Kelly, M.E. Webb, S.M. |
| author_facet | Pushie, M.J. Sylvain, N.J. Hou, H. Hackett, Mark Kelly, M.E. Webb, S.M. |
| author_sort | Pushie, M.J. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | Synchrotron-based X-ray fluorescence microscopy is a flexible tool for identifying the distribution of trace elements in biological specimens across a broad range of sample sizes. The technique is not particularly limited by sample type and can be performed on ancient fossils, fixed or fresh tissue specimens, and in some cases even live tissue and live cells can be studied. The technique can also be expanded to provide chemical specificity to elemental maps, either at individual points of interest in a map or across a large field of view. While virtually any sample type can be characterized with X-ray fluorescence microscopy, common biological sample preparation methods ( often borrowed from other fields, such as histology ) can lead to unforeseen pitfalls, resulting in altered element distributions and concentrations. A general overview of sample preparation and data-acquisition methods for X-ray fluorescence microscopy is presented, along with outlining the general approach for applying this technique to a new field of investigation for prospective new users. Considerations for improving data acquisition and quality are reviewed as well as the effects of sample prepa- ration, with a particular focus on soft tissues. The effects of common sample pretreatment steps as well as the underlying factors that govern which, and to what extent, specific elements are likely to be altered are reviewed along with common artifacts observed in X-ray fluorescence microscopy data. |
| first_indexed | 2025-11-14T11:39:08Z |
| format | Journal Article |
| id | curtin-20.500.11937-92982 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T11:39:08Z |
| publishDate | 2022 |
| publisher | OXFORD UNIV PRESS |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-929822023-08-28T01:18:10Z X-ray fluorescence microscopy methods for biological tissues Pushie, M.J. Sylvain, N.J. Hou, H. Hackett, Mark Kelly, M.E. Webb, S.M. Science & Technology Life Sciences & Biomedicine Biochemistry & Molecular Biology X-ray fluorescence microscopy mapping imaging XFI XFM biological samples chemical speciation ALZHEIMERS-DISEASE K-EDGE COPPER MOUSE TRANSMISSION SCRAPIE IRON CU MICROANALYSIS MECHANISMS X-ray fluorescence microscopy XFI XFM biological samples chemical speciation imaging mapping Microscopy, Fluorescence Prospective Studies Spectrometry, X-Ray Emission Synchrotrons Trace Elements X-Rays Trace Elements Microscopy, Fluorescence Spectrometry, X-Ray Emission Prospective Studies Synchrotrons X-Rays Synchrotron-based X-ray fluorescence microscopy is a flexible tool for identifying the distribution of trace elements in biological specimens across a broad range of sample sizes. The technique is not particularly limited by sample type and can be performed on ancient fossils, fixed or fresh tissue specimens, and in some cases even live tissue and live cells can be studied. The technique can also be expanded to provide chemical specificity to elemental maps, either at individual points of interest in a map or across a large field of view. While virtually any sample type can be characterized with X-ray fluorescence microscopy, common biological sample preparation methods ( often borrowed from other fields, such as histology ) can lead to unforeseen pitfalls, resulting in altered element distributions and concentrations. A general overview of sample preparation and data-acquisition methods for X-ray fluorescence microscopy is presented, along with outlining the general approach for applying this technique to a new field of investigation for prospective new users. Considerations for improving data acquisition and quality are reviewed as well as the effects of sample prepa- ration, with a particular focus on soft tissues. The effects of common sample pretreatment steps as well as the underlying factors that govern which, and to what extent, specific elements are likely to be altered are reviewed along with common artifacts observed in X-ray fluorescence microscopy data. 2022 Journal Article http://hdl.handle.net/20.500.11937/92982 10.1093/mtomcs/mfac032 English OXFORD UNIV PRESS unknown |
| spellingShingle | Science & Technology Life Sciences & Biomedicine Biochemistry & Molecular Biology X-ray fluorescence microscopy mapping imaging XFI XFM biological samples chemical speciation ALZHEIMERS-DISEASE K-EDGE COPPER MOUSE TRANSMISSION SCRAPIE IRON CU MICROANALYSIS MECHANISMS X-ray fluorescence microscopy XFI XFM biological samples chemical speciation imaging mapping Microscopy, Fluorescence Prospective Studies Spectrometry, X-Ray Emission Synchrotrons Trace Elements X-Rays Trace Elements Microscopy, Fluorescence Spectrometry, X-Ray Emission Prospective Studies Synchrotrons X-Rays Pushie, M.J. Sylvain, N.J. Hou, H. Hackett, Mark Kelly, M.E. Webb, S.M. X-ray fluorescence microscopy methods for biological tissues |
| title | X-ray fluorescence microscopy methods for biological tissues |
| title_full | X-ray fluorescence microscopy methods for biological tissues |
| title_fullStr | X-ray fluorescence microscopy methods for biological tissues |
| title_full_unstemmed | X-ray fluorescence microscopy methods for biological tissues |
| title_short | X-ray fluorescence microscopy methods for biological tissues |
| title_sort | x-ray fluorescence microscopy methods for biological tissues |
| topic | Science & Technology Life Sciences & Biomedicine Biochemistry & Molecular Biology X-ray fluorescence microscopy mapping imaging XFI XFM biological samples chemical speciation ALZHEIMERS-DISEASE K-EDGE COPPER MOUSE TRANSMISSION SCRAPIE IRON CU MICROANALYSIS MECHANISMS X-ray fluorescence microscopy XFI XFM biological samples chemical speciation imaging mapping Microscopy, Fluorescence Prospective Studies Spectrometry, X-Ray Emission Synchrotrons Trace Elements X-Rays Trace Elements Microscopy, Fluorescence Spectrometry, X-Ray Emission Prospective Studies Synchrotrons X-Rays |
| url | http://hdl.handle.net/20.500.11937/92982 |