Multivariate analysis of otolith microchemistry can discriminate the source of oil contamination in exposed fish
The uptake of metals into the aragonite lattice of the fish otolith (ear-bone) has been used for decades as a historical record of exposure to metals in polluted environments. The relative abundance of two metals in particular, Ni and V, are used in forensic chemical analysis of crude oils to assist...
| Main Authors: | , , , , , |
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| Format: | Journal Article |
| Language: | English |
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ELSEVIER SCIENCE INC
2022
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| Subjects: | |
| Online Access: | http://purl.org/au-research/grants/arc/LP170101000 http://hdl.handle.net/20.500.11937/91741 |
| _version_ | 1848765583713107968 |
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| author | Spilsbury, F. McDonald, B. Rankenburg, Kai Evans, Noreen Grice, Kliti Gagnon, Monique |
| author_facet | Spilsbury, F. McDonald, B. Rankenburg, Kai Evans, Noreen Grice, Kliti Gagnon, Monique |
| author_sort | Spilsbury, F. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | The uptake of metals into the aragonite lattice of the fish otolith (ear-bone) has been used for decades as a historical record of exposure to metals in polluted environments. The relative abundance of two metals in particular, Ni and V, are used in forensic chemical analysis of crude oils to assist in confirming its origin. In this study we investigate the potential for metal accumulation in otoliths to act as a biomarker of exposure to crude oil. Using a 33-day static-renewal laboratory trial design, 56 juvenile Lates calcarifer (commonly known as Asian seabass or barramundi) were fed diets enriched with V (20 mg/kg), Ni (500 mg/kg), Fe (500 mg/kg), and two crude oils with distinctly different metals profiles: a heavy fuel oil (1% w/w) and a typical Australian medium crude (1% w/w). Fish exposed to crude oils showed Ba and Al retained in otoliths in a dose-dependent manner, but fish fed V-, Ni- and Fe-enriched diets showed no metal increase in otoliths, indicating that V, Ni and Fe are not incorporated into the otolith of L. calcarifer via dietary exposure. For crude oils, incorporation into otolith for many metals is likely limited due to porphyrin casing reducing their bioavailability. Principal components analysis (PCA) and subsequent linear discriminatory analysis (LDA) of selected otolith metals demonstrated that, even despite large variability in the metal abundances detected in otolith between individuals within the test groups (cv = 1.00), it is possible to discriminate between fish exposed to different crude oils using multivariate analysis of their otolith microchemistry. |
| first_indexed | 2025-11-14T11:37:34Z |
| format | Journal Article |
| id | curtin-20.500.11937-91741 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T11:37:34Z |
| publishDate | 2022 |
| publisher | ELSEVIER SCIENCE INC |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-917412023-06-01T09:30:54Z Multivariate analysis of otolith microchemistry can discriminate the source of oil contamination in exposed fish Spilsbury, F. McDonald, B. Rankenburg, Kai Evans, Noreen Grice, Kliti Gagnon, Monique Science & Technology Life Sciences & Biomedicine Biochemistry & Molecular Biology Endocrinology & Metabolism Toxicology Zoology Otolith Vanadium Nickel Crude oil Heavy fuel oil PCA LDA CRUDE-OIL PETROLEUM-HYDROCARBONS TRACE-METALS ICP-MS NICKEL GULF BARRAMUNDI INDICATORS INGESTION CHEMISTRY Crude oil Heavy fuel oil LDA Nickel Otolith PCA Vanadium Animals Australia Fishes Microchemistry Multivariate Analysis Otolithic Membrane Otolithic Membrane Animals Fishes Microchemistry Multivariate Analysis Australia The uptake of metals into the aragonite lattice of the fish otolith (ear-bone) has been used for decades as a historical record of exposure to metals in polluted environments. The relative abundance of two metals in particular, Ni and V, are used in forensic chemical analysis of crude oils to assist in confirming its origin. In this study we investigate the potential for metal accumulation in otoliths to act as a biomarker of exposure to crude oil. Using a 33-day static-renewal laboratory trial design, 56 juvenile Lates calcarifer (commonly known as Asian seabass or barramundi) were fed diets enriched with V (20 mg/kg), Ni (500 mg/kg), Fe (500 mg/kg), and two crude oils with distinctly different metals profiles: a heavy fuel oil (1% w/w) and a typical Australian medium crude (1% w/w). Fish exposed to crude oils showed Ba and Al retained in otoliths in a dose-dependent manner, but fish fed V-, Ni- and Fe-enriched diets showed no metal increase in otoliths, indicating that V, Ni and Fe are not incorporated into the otolith of L. calcarifer via dietary exposure. For crude oils, incorporation into otolith for many metals is likely limited due to porphyrin casing reducing their bioavailability. Principal components analysis (PCA) and subsequent linear discriminatory analysis (LDA) of selected otolith metals demonstrated that, even despite large variability in the metal abundances detected in otolith between individuals within the test groups (cv = 1.00), it is possible to discriminate between fish exposed to different crude oils using multivariate analysis of their otolith microchemistry. 2022 Journal Article http://hdl.handle.net/20.500.11937/91741 10.1016/j.cbpc.2021.109253 English http://purl.org/au-research/grants/arc/LP170101000 ELSEVIER SCIENCE INC fulltext |
| spellingShingle | Science & Technology Life Sciences & Biomedicine Biochemistry & Molecular Biology Endocrinology & Metabolism Toxicology Zoology Otolith Vanadium Nickel Crude oil Heavy fuel oil PCA LDA CRUDE-OIL PETROLEUM-HYDROCARBONS TRACE-METALS ICP-MS NICKEL GULF BARRAMUNDI INDICATORS INGESTION CHEMISTRY Crude oil Heavy fuel oil LDA Nickel Otolith PCA Vanadium Animals Australia Fishes Microchemistry Multivariate Analysis Otolithic Membrane Otolithic Membrane Animals Fishes Microchemistry Multivariate Analysis Australia Spilsbury, F. McDonald, B. Rankenburg, Kai Evans, Noreen Grice, Kliti Gagnon, Monique Multivariate analysis of otolith microchemistry can discriminate the source of oil contamination in exposed fish |
| title | Multivariate analysis of otolith microchemistry can discriminate the source of oil contamination in exposed fish |
| title_full | Multivariate analysis of otolith microchemistry can discriminate the source of oil contamination in exposed fish |
| title_fullStr | Multivariate analysis of otolith microchemistry can discriminate the source of oil contamination in exposed fish |
| title_full_unstemmed | Multivariate analysis of otolith microchemistry can discriminate the source of oil contamination in exposed fish |
| title_short | Multivariate analysis of otolith microchemistry can discriminate the source of oil contamination in exposed fish |
| title_sort | multivariate analysis of otolith microchemistry can discriminate the source of oil contamination in exposed fish |
| topic | Science & Technology Life Sciences & Biomedicine Biochemistry & Molecular Biology Endocrinology & Metabolism Toxicology Zoology Otolith Vanadium Nickel Crude oil Heavy fuel oil PCA LDA CRUDE-OIL PETROLEUM-HYDROCARBONS TRACE-METALS ICP-MS NICKEL GULF BARRAMUNDI INDICATORS INGESTION CHEMISTRY Crude oil Heavy fuel oil LDA Nickel Otolith PCA Vanadium Animals Australia Fishes Microchemistry Multivariate Analysis Otolithic Membrane Otolithic Membrane Animals Fishes Microchemistry Multivariate Analysis Australia |
| url | http://purl.org/au-research/grants/arc/LP170101000 http://hdl.handle.net/20.500.11937/91741 |