Cryopreservation of the Australian species Macropidia fuliginosa (Haemodoraceae) by vitrification
Somatic embryos were used to develop a cryopreservation protocol for Macropidia fuliginosa, a commercially-important species endemic to the south-west of Western Australia. Somatic embryos were allowed to develop from embryogenic callus for three weeks on an kinetin medium prior to processing. These...
| Main Authors: | , , , , , |
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| Format: | Journal Article |
| Language: | English |
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CRYO LETTERS
2000
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| Online Access: | http://hdl.handle.net/20.500.11937/91302 |
| _version_ | 1848765501636870144 |
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| author | Turner, Shane Tan, B. Senaratna, T. Bunn, E. Dixon, Kingsley Touchell, D.H. |
| author_facet | Turner, Shane Tan, B. Senaratna, T. Bunn, E. Dixon, Kingsley Touchell, D.H. |
| author_sort | Turner, Shane |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | Somatic embryos were used to develop a cryopreservation protocol for Macropidia fuliginosa, a commercially-important species endemic to the south-west of Western Australia. Somatic embryos were allowed to develop from embryogenic callus for three weeks on an kinetin medium prior to processing. These were transferred and cultured on a agar solidified basal medium supplemented with 0 to 0.6 M sorbitol for 2 d prior to incubation in Plant Vitrification Solution Two (PVS2). Following this, embryos were then washed in 1 M sucrose solution (treated controls) or cooled in liquid nitrogen (LN). Cooled embryos were then warmed and washed in sucrose solution. Highest survival for cooled treatments (67.3%) was achieved by preculture with 0.4 M sorbitol, then incubation in PVS2. Further experimentation varying pre-culture duration (2 or 3 d) and incubation on either glycerol (0.8 M) or sorbitol (0.4 M) indicated that very high survival (90.6%) of embryos was achievable by adopting a 2 d preculture period on 0.8 M glycerol. The phenotype and growth rates of plants obtained using this protocol were similar to those of parent plants. This optimised procedure was then applied to tissue culture-derived shoot apices of the same clone also resulting in a high survival rate (84.4%). |
| first_indexed | 2025-11-14T11:36:15Z |
| format | Journal Article |
| id | curtin-20.500.11937-91302 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T11:36:15Z |
| publishDate | 2000 |
| publisher | CRYO LETTERS |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-913022023-04-19T06:50:16Z Cryopreservation of the Australian species Macropidia fuliginosa (Haemodoraceae) by vitrification Turner, Shane Tan, B. Senaratna, T. Bunn, E. Dixon, Kingsley Touchell, D.H. Science & Technology Life Sciences & Biomedicine Biology Physiology Life Sciences & Biomedicine - Other Topics Macropidia fuliginosa PVS2 sorbitol glycerol somatic embryos SHOOT TIPS SURVIVAL CELLS Somatic embryos were used to develop a cryopreservation protocol for Macropidia fuliginosa, a commercially-important species endemic to the south-west of Western Australia. Somatic embryos were allowed to develop from embryogenic callus for three weeks on an kinetin medium prior to processing. These were transferred and cultured on a agar solidified basal medium supplemented with 0 to 0.6 M sorbitol for 2 d prior to incubation in Plant Vitrification Solution Two (PVS2). Following this, embryos were then washed in 1 M sucrose solution (treated controls) or cooled in liquid nitrogen (LN). Cooled embryos were then warmed and washed in sucrose solution. Highest survival for cooled treatments (67.3%) was achieved by preculture with 0.4 M sorbitol, then incubation in PVS2. Further experimentation varying pre-culture duration (2 or 3 d) and incubation on either glycerol (0.8 M) or sorbitol (0.4 M) indicated that very high survival (90.6%) of embryos was achievable by adopting a 2 d preculture period on 0.8 M glycerol. The phenotype and growth rates of plants obtained using this protocol were similar to those of parent plants. This optimised procedure was then applied to tissue culture-derived shoot apices of the same clone also resulting in a high survival rate (84.4%). 2000 Journal Article http://hdl.handle.net/20.500.11937/91302 English CRYO LETTERS restricted |
| spellingShingle | Science & Technology Life Sciences & Biomedicine Biology Physiology Life Sciences & Biomedicine - Other Topics Macropidia fuliginosa PVS2 sorbitol glycerol somatic embryos SHOOT TIPS SURVIVAL CELLS Turner, Shane Tan, B. Senaratna, T. Bunn, E. Dixon, Kingsley Touchell, D.H. Cryopreservation of the Australian species Macropidia fuliginosa (Haemodoraceae) by vitrification |
| title | Cryopreservation of the Australian species Macropidia fuliginosa (Haemodoraceae) by vitrification |
| title_full | Cryopreservation of the Australian species Macropidia fuliginosa (Haemodoraceae) by vitrification |
| title_fullStr | Cryopreservation of the Australian species Macropidia fuliginosa (Haemodoraceae) by vitrification |
| title_full_unstemmed | Cryopreservation of the Australian species Macropidia fuliginosa (Haemodoraceae) by vitrification |
| title_short | Cryopreservation of the Australian species Macropidia fuliginosa (Haemodoraceae) by vitrification |
| title_sort | cryopreservation of the australian species macropidia fuliginosa (haemodoraceae) by vitrification |
| topic | Science & Technology Life Sciences & Biomedicine Biology Physiology Life Sciences & Biomedicine - Other Topics Macropidia fuliginosa PVS2 sorbitol glycerol somatic embryos SHOOT TIPS SURVIVAL CELLS |
| url | http://hdl.handle.net/20.500.11937/91302 |