Effects of plant growth regulators on survival and recovery growth following cryopreservation
Studies on the effects of plant growth regulators (PGRs) on survival, recovery and postrecovery growth of shoot apices following cryopreservation are limited. In this study, the effects of plant growth regulators in both the culture phase and the recovery phase of cryostorage were examined for the r...
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| Format: | Journal Article |
| Language: | English |
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CRYO LETTERS
2001
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| Subjects: | |
| Online Access: | http://hdl.handle.net/20.500.11937/91301 |
| _version_ | 1848765501372628992 |
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| author | Turner, Shane Touchell, D.H. Senaratna, T. Bunn, E. Tan, B. Dixon, Kingsley |
| author_facet | Turner, Shane Touchell, D.H. Senaratna, T. Bunn, E. Tan, B. Dixon, Kingsley |
| author_sort | Turner, Shane |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | Studies on the effects of plant growth regulators (PGRs) on survival, recovery and postrecovery growth of shoot apices following cryopreservation are limited. In this study, the effects of plant growth regulators in both the culture phase and the recovery phase of cryostorage were examined for the rare plant species, Anigozanthos viridis ssp terraspectans Hopper. Survival of shoot apices was not correlated to cytokinin or auxin treatments administered in culture media prior to cryostorage. In recovery media, the plant growth regulators, kinetin, zeatin (cytokinins), IAA, (auxin) and GA3 were examined for their effect following cryopreservation. It was found that the application of a combination of cytokinin and 0.5 μM GA3 from day zero was the most appropriate for obtaining vigorously growing plantlets following LN immersion. This combination proved to be more effective than basal medium, zeatin or kinetin treatments. |
| first_indexed | 2025-11-14T11:36:15Z |
| format | Journal Article |
| id | curtin-20.500.11937-91301 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T11:36:15Z |
| publishDate | 2001 |
| publisher | CRYO LETTERS |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-913012023-04-19T06:51:42Z Effects of plant growth regulators on survival and recovery growth following cryopreservation Turner, Shane Touchell, D.H. Senaratna, T. Bunn, E. Tan, B. Dixon, Kingsley Science & Technology Life Sciences & Biomedicine Biology Physiology Life Sciences & Biomedicine - Other Topics Anigozanthos viridis ssp terraspectans plant growth regulators (PGR) cryopreservation PVS2 vitrification rare and endangered SHOOT-TIPS WESTERN-AUSTRALIA INVITRO-PROPAGATION COLD-ACCLIMATION VITRIFICATION Cryopreservation Culture Media, Conditioned Cytokinins Plant Growth Regulators Plant Physiological Phenomena Plant Shoots Regeneration Survival Rate Time Factors Plant Shoots Plant Growth Regulators Cytokinins Culture Media, Conditioned Cryopreservation Survival Rate Regeneration Time Factors Plant Physiological Phenomena Studies on the effects of plant growth regulators (PGRs) on survival, recovery and postrecovery growth of shoot apices following cryopreservation are limited. In this study, the effects of plant growth regulators in both the culture phase and the recovery phase of cryostorage were examined for the rare plant species, Anigozanthos viridis ssp terraspectans Hopper. Survival of shoot apices was not correlated to cytokinin or auxin treatments administered in culture media prior to cryostorage. In recovery media, the plant growth regulators, kinetin, zeatin (cytokinins), IAA, (auxin) and GA3 were examined for their effect following cryopreservation. It was found that the application of a combination of cytokinin and 0.5 μM GA3 from day zero was the most appropriate for obtaining vigorously growing plantlets following LN immersion. This combination proved to be more effective than basal medium, zeatin or kinetin treatments. 2001 Journal Article http://hdl.handle.net/20.500.11937/91301 English CRYO LETTERS restricted |
| spellingShingle | Science & Technology Life Sciences & Biomedicine Biology Physiology Life Sciences & Biomedicine - Other Topics Anigozanthos viridis ssp terraspectans plant growth regulators (PGR) cryopreservation PVS2 vitrification rare and endangered SHOOT-TIPS WESTERN-AUSTRALIA INVITRO-PROPAGATION COLD-ACCLIMATION VITRIFICATION Cryopreservation Culture Media, Conditioned Cytokinins Plant Growth Regulators Plant Physiological Phenomena Plant Shoots Regeneration Survival Rate Time Factors Plant Shoots Plant Growth Regulators Cytokinins Culture Media, Conditioned Cryopreservation Survival Rate Regeneration Time Factors Plant Physiological Phenomena Turner, Shane Touchell, D.H. Senaratna, T. Bunn, E. Tan, B. Dixon, Kingsley Effects of plant growth regulators on survival and recovery growth following cryopreservation |
| title | Effects of plant growth regulators on survival and recovery growth following cryopreservation |
| title_full | Effects of plant growth regulators on survival and recovery growth following cryopreservation |
| title_fullStr | Effects of plant growth regulators on survival and recovery growth following cryopreservation |
| title_full_unstemmed | Effects of plant growth regulators on survival and recovery growth following cryopreservation |
| title_short | Effects of plant growth regulators on survival and recovery growth following cryopreservation |
| title_sort | effects of plant growth regulators on survival and recovery growth following cryopreservation |
| topic | Science & Technology Life Sciences & Biomedicine Biology Physiology Life Sciences & Biomedicine - Other Topics Anigozanthos viridis ssp terraspectans plant growth regulators (PGR) cryopreservation PVS2 vitrification rare and endangered SHOOT-TIPS WESTERN-AUSTRALIA INVITRO-PROPAGATION COLD-ACCLIMATION VITRIFICATION Cryopreservation Culture Media, Conditioned Cytokinins Plant Growth Regulators Plant Physiological Phenomena Plant Shoots Regeneration Survival Rate Time Factors Plant Shoots Plant Growth Regulators Cytokinins Culture Media, Conditioned Cryopreservation Survival Rate Regeneration Time Factors Plant Physiological Phenomena |
| url | http://hdl.handle.net/20.500.11937/91301 |