Current issues in plant cryopreservation and importance for ex situ conservation of threatened Australian native species
An alarming proportion of Australia's unique plant biodiversity is under siege from a variety of environmental threats. Options for in situ conservation are becoming increasingly compromised as encroaching land use, climate change and introduced diseases are highly likely to erode sanctuaries r...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Journal Article |
| Language: | English |
| Published: |
CSIRO PUBLISHING
2019
|
| Subjects: | |
| Online Access: | http://purl.org/au-research/grants/arc/LP160101496 http://hdl.handle.net/20.500.11937/90907 |
| _version_ | 1848765457326145536 |
|---|---|
| author | Streczynski, Robyn Clark, Hamish Whelehan, Lily M. Ang, Sze-Tieng Hardstaff, Lyndle K. Funnekotter, Bryn Bunn, Eric Offord, C.A. Sommerville, K.D. Mancera, Ricardo |
| author_facet | Streczynski, Robyn Clark, Hamish Whelehan, Lily M. Ang, Sze-Tieng Hardstaff, Lyndle K. Funnekotter, Bryn Bunn, Eric Offord, C.A. Sommerville, K.D. Mancera, Ricardo |
| author_sort | Streczynski, Robyn |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | An alarming proportion of Australia's unique plant biodiversity is under siege from a variety of environmental threats. Options for in situ conservation are becoming increasingly compromised as encroaching land use, climate change and introduced diseases are highly likely to erode sanctuaries regardless of best intentions. Ex situ conservation is currently limited to botanic garden living collections and seed banking, with in vitro and cryopreservation technologies still being developed to address ex situ conservation of species not amenable to conventional storage. Cryopreservation (storage in liquid nitrogen) has been used successfully for long-term biosecure storage of shoot tips of several species of threatened Australian plants. We present a case for building on this research and fostering further development and utilisation of cryopreservation as the best means of capturing critical germplasm collections of Australian species with special storage requirements (e.g. recalcitrant-seeded taxa and species with short-lived seeds) that currently cannot be preserved effectively by other means. This review highlights the major issues in cryopreservation that can limit survival including ice crystal damage and desiccation, toxicity of cryoprotective agents, membrane damage, oxidative stress and mitochondrial function. Progress in understanding and mitigating these stresses is vital for advancing cryopreservation for conservation purposes. |
| first_indexed | 2025-11-14T11:35:33Z |
| format | Journal Article |
| id | curtin-20.500.11937-90907 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T11:35:33Z |
| publishDate | 2019 |
| publisher | CSIRO PUBLISHING |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-909072023-05-01T07:55:28Z Current issues in plant cryopreservation and importance for ex situ conservation of threatened Australian native species Streczynski, Robyn Clark, Hamish Whelehan, Lily M. Ang, Sze-Tieng Hardstaff, Lyndle K. Funnekotter, Bryn Bunn, Eric Offord, C.A. Sommerville, K.D. Mancera, Ricardo Science & Technology Life Sciences & Biomedicine Plant Sciences cryobank cryobiology cryobiotechnology cryostorage freezing in vitro conservation plant tissue culture vitrification MOLECULAR-DYNAMICS SIMULATIONS BETA-SITOSTEROL BILAYERS LIQUID-NITROGEN EXPOSURE SHOOT-TIPS OXIDATIVE STRESS EMBRYONIC AXES ENCAPSULATION-DEHYDRATION RECALCITRANT SEEDS COLD-ACCLIMATION MYRTLE RUST An alarming proportion of Australia's unique plant biodiversity is under siege from a variety of environmental threats. Options for in situ conservation are becoming increasingly compromised as encroaching land use, climate change and introduced diseases are highly likely to erode sanctuaries regardless of best intentions. Ex situ conservation is currently limited to botanic garden living collections and seed banking, with in vitro and cryopreservation technologies still being developed to address ex situ conservation of species not amenable to conventional storage. Cryopreservation (storage in liquid nitrogen) has been used successfully for long-term biosecure storage of shoot tips of several species of threatened Australian plants. We present a case for building on this research and fostering further development and utilisation of cryopreservation as the best means of capturing critical germplasm collections of Australian species with special storage requirements (e.g. recalcitrant-seeded taxa and species with short-lived seeds) that currently cannot be preserved effectively by other means. This review highlights the major issues in cryopreservation that can limit survival including ice crystal damage and desiccation, toxicity of cryoprotective agents, membrane damage, oxidative stress and mitochondrial function. Progress in understanding and mitigating these stresses is vital for advancing cryopreservation for conservation purposes. 2019 Journal Article http://hdl.handle.net/20.500.11937/90907 10.1071/BT18147 English http://purl.org/au-research/grants/arc/LP160101496 CSIRO PUBLISHING unknown |
| spellingShingle | Science & Technology Life Sciences & Biomedicine Plant Sciences cryobank cryobiology cryobiotechnology cryostorage freezing in vitro conservation plant tissue culture vitrification MOLECULAR-DYNAMICS SIMULATIONS BETA-SITOSTEROL BILAYERS LIQUID-NITROGEN EXPOSURE SHOOT-TIPS OXIDATIVE STRESS EMBRYONIC AXES ENCAPSULATION-DEHYDRATION RECALCITRANT SEEDS COLD-ACCLIMATION MYRTLE RUST Streczynski, Robyn Clark, Hamish Whelehan, Lily M. Ang, Sze-Tieng Hardstaff, Lyndle K. Funnekotter, Bryn Bunn, Eric Offord, C.A. Sommerville, K.D. Mancera, Ricardo Current issues in plant cryopreservation and importance for ex situ conservation of threatened Australian native species |
| title | Current issues in plant cryopreservation and importance for ex situ conservation of threatened Australian native species |
| title_full | Current issues in plant cryopreservation and importance for ex situ conservation of threatened Australian native species |
| title_fullStr | Current issues in plant cryopreservation and importance for ex situ conservation of threatened Australian native species |
| title_full_unstemmed | Current issues in plant cryopreservation and importance for ex situ conservation of threatened Australian native species |
| title_short | Current issues in plant cryopreservation and importance for ex situ conservation of threatened Australian native species |
| title_sort | current issues in plant cryopreservation and importance for ex situ conservation of threatened australian native species |
| topic | Science & Technology Life Sciences & Biomedicine Plant Sciences cryobank cryobiology cryobiotechnology cryostorage freezing in vitro conservation plant tissue culture vitrification MOLECULAR-DYNAMICS SIMULATIONS BETA-SITOSTEROL BILAYERS LIQUID-NITROGEN EXPOSURE SHOOT-TIPS OXIDATIVE STRESS EMBRYONIC AXES ENCAPSULATION-DEHYDRATION RECALCITRANT SEEDS COLD-ACCLIMATION MYRTLE RUST |
| url | http://purl.org/au-research/grants/arc/LP160101496 http://hdl.handle.net/20.500.11937/90907 |