Luciferase-free Luciferin Electrochemiluminescence
Luciferin is one of Nature's most widespread luminophores, and enzymes that catalyze luciferin luminescence are the basis of successful commercial “glow” assays for gene expression and metabolic ATP formation. Herein we report an electrochemical method to promote firefly's luciferin lumine...
| Main Authors: | , , , , , , , |
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| Format: | Journal Article |
| Language: | English |
| Published: |
WILEY-V C H VERLAG GMBH
2022
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| Online Access: | http://purl.org/au-research/grants/arc/DP190100735 http://hdl.handle.net/20.500.11937/90478 |
| _version_ | 1848765385058287616 |
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| author | Belotti, Mattia El-Tahawy, M.M.T. Yu, L.J. Russell, I.C. Darwish, Nadim Coote, M.L. Garavelli, M. Ciampi, Simone |
| author_facet | Belotti, Mattia El-Tahawy, M.M.T. Yu, L.J. Russell, I.C. Darwish, Nadim Coote, M.L. Garavelli, M. Ciampi, Simone |
| author_sort | Belotti, Mattia |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | Luciferin is one of Nature's most widespread luminophores, and enzymes that catalyze luciferin luminescence are the basis of successful commercial “glow” assays for gene expression and metabolic ATP formation. Herein we report an electrochemical method to promote firefly's luciferin luminescence in the absence of its natural biocatalyst—luciferase. We have gained experimental and computational insights on the mechanism of the enzyme-free luciferin electrochemiluminescence, demonstrated its spectral tuning from green to red by means of electrolyte engineering, proven that the colour change does not require, as still debated, a keto/enol isomerization of the light emitter, and gained evidence of the electrostatic-assisted stabilization of the charge-transfer excited state by double layer electric fields. Luciferin's electrochemiluminescence, as well as the in situ generation of fluorescent oxyluciferin, are applied towards an optical measurement of diffusion coefficients. |
| first_indexed | 2025-11-14T11:34:24Z |
| format | Journal Article |
| id | curtin-20.500.11937-90478 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T11:34:24Z |
| publishDate | 2022 |
| publisher | WILEY-V C H VERLAG GMBH |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-904782023-03-20T04:50:06Z Luciferase-free Luciferin Electrochemiluminescence Belotti, Mattia El-Tahawy, M.M.T. Yu, L.J. Russell, I.C. Darwish, Nadim Coote, M.L. Garavelli, M. Ciampi, Simone Science & Technology Physical Sciences Chemistry, Multidisciplinary Chemistry Electrochemiluminescence Electrochemistry Electrostatic Interactions Profluorescence Quantum Chemistry ELECTRICAL DOUBLE-LAYER FIREFLY LUCIFERIN SPECTRAL DISTRIBUTION BIOLUMINESCENCE CHEMILUMINESCENCE MECHANISM MODULATION LIGHT LUMINESCENCE OXIDATION Luciferin is one of Nature's most widespread luminophores, and enzymes that catalyze luciferin luminescence are the basis of successful commercial “glow” assays for gene expression and metabolic ATP formation. Herein we report an electrochemical method to promote firefly's luciferin luminescence in the absence of its natural biocatalyst—luciferase. We have gained experimental and computational insights on the mechanism of the enzyme-free luciferin electrochemiluminescence, demonstrated its spectral tuning from green to red by means of electrolyte engineering, proven that the colour change does not require, as still debated, a keto/enol isomerization of the light emitter, and gained evidence of the electrostatic-assisted stabilization of the charge-transfer excited state by double layer electric fields. Luciferin's electrochemiluminescence, as well as the in situ generation of fluorescent oxyluciferin, are applied towards an optical measurement of diffusion coefficients. 2022 Journal Article http://hdl.handle.net/20.500.11937/90478 10.1002/anie.202209670 English http://purl.org/au-research/grants/arc/DP190100735 http://purl.org/au-research/grants/arc/FT190100148 http://purl.org/au-research/grants/arc/FL170100041 http://purl.org/au-research/grants/arc/CE140100012 http://creativecommons.org/licenses/by-nc-nd/4.0/ WILEY-V C H VERLAG GMBH fulltext |
| spellingShingle | Science & Technology Physical Sciences Chemistry, Multidisciplinary Chemistry Electrochemiluminescence Electrochemistry Electrostatic Interactions Profluorescence Quantum Chemistry ELECTRICAL DOUBLE-LAYER FIREFLY LUCIFERIN SPECTRAL DISTRIBUTION BIOLUMINESCENCE CHEMILUMINESCENCE MECHANISM MODULATION LIGHT LUMINESCENCE OXIDATION Belotti, Mattia El-Tahawy, M.M.T. Yu, L.J. Russell, I.C. Darwish, Nadim Coote, M.L. Garavelli, M. Ciampi, Simone Luciferase-free Luciferin Electrochemiluminescence |
| title | Luciferase-free Luciferin Electrochemiluminescence |
| title_full | Luciferase-free Luciferin Electrochemiluminescence |
| title_fullStr | Luciferase-free Luciferin Electrochemiluminescence |
| title_full_unstemmed | Luciferase-free Luciferin Electrochemiluminescence |
| title_short | Luciferase-free Luciferin Electrochemiluminescence |
| title_sort | luciferase-free luciferin electrochemiluminescence |
| topic | Science & Technology Physical Sciences Chemistry, Multidisciplinary Chemistry Electrochemiluminescence Electrochemistry Electrostatic Interactions Profluorescence Quantum Chemistry ELECTRICAL DOUBLE-LAYER FIREFLY LUCIFERIN SPECTRAL DISTRIBUTION BIOLUMINESCENCE CHEMILUMINESCENCE MECHANISM MODULATION LIGHT LUMINESCENCE OXIDATION |
| url | http://purl.org/au-research/grants/arc/DP190100735 http://purl.org/au-research/grants/arc/DP190100735 http://purl.org/au-research/grants/arc/DP190100735 http://purl.org/au-research/grants/arc/DP190100735 http://hdl.handle.net/20.500.11937/90478 |