“Wax On, Wax Off”: In Vivo Imaging of Plant Physiology and Disease with Fourier Transform Infrared Reflectance Microspectroscopy
Analysis of the epicuticular wax layer on the surface of plant leaves can provide a unique window into plant physiology and responses to environmental stimuli. Well-established analytical methodologies can quantify epicuticular wax composition, yet few methods are capable of imaging wax distribution...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Journal Article |
| Language: | English |
| Published: |
WILEY
2021
|
| Subjects: | |
| Online Access: | http://purl.org/au-research/grants/arc/FT190100017 http://hdl.handle.net/20.500.11937/90105 |
| _version_ | 1848765326352711680 |
|---|---|
| author | Khambatta, Karina Hollings, Ashley Sauzier, Georgina Sanglard, Lilian M.V.P. Klein, A.R. Tobin, M.J. Vongsvivut, J. Gibberd, Mark Payne, Alan Naim, Fatima Hackett, Mark |
| author_facet | Khambatta, Karina Hollings, Ashley Sauzier, Georgina Sanglard, Lilian M.V.P. Klein, A.R. Tobin, M.J. Vongsvivut, J. Gibberd, Mark Payne, Alan Naim, Fatima Hackett, Mark |
| author_sort | Khambatta, Karina |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | Analysis of the epicuticular wax layer on the surface of plant leaves can provide a unique window into plant physiology and responses to environmental stimuli. Well-established analytical methodologies can quantify epicuticular wax composition, yet few methods are capable of imaging wax distribution in situ or in vivo. Here, the first report of Fourier transform infrared (FTIR) reflectance spectroscopic imaging as a non-destructive, in situ, method to investigate variation in epicuticular wax distribution at 25 µm spatial resolution is presented. The authors demonstrate in vivo imaging of alterations in epicuticular waxes during leaf development and in situ imaging during plant disease or exposure to environmental stressors. It is envisaged that this new analytical capability will enable in vivo studies of plants to provide insights into how the physiology of plants and crops respond to environmental stresses such as disease, soil contamination, drought, soil acidity, and climate change. |
| first_indexed | 2025-11-14T11:33:28Z |
| format | Journal Article |
| id | curtin-20.500.11937-90105 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T11:33:28Z |
| publishDate | 2021 |
| publisher | WILEY |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-901052023-02-13T06:12:09Z “Wax On, Wax Off”: In Vivo Imaging of Plant Physiology and Disease with Fourier Transform Infrared Reflectance Microspectroscopy Khambatta, Karina Hollings, Ashley Sauzier, Georgina Sanglard, Lilian M.V.P. Klein, A.R. Tobin, M.J. Vongsvivut, J. Gibberd, Mark Payne, Alan Naim, Fatima Hackett, Mark Science & Technology Physical Sciences Technology Chemistry, Multidisciplinary Nanoscience & Nanotechnology Materials Science, Multidisciplinary Chemistry Science & Technology - Other Topics Materials Science epicuticular wax infrared microscopy plant physiology reflectance SPECTROSCOPY RAMAN STRESS ATR INTERFACE SURFACES Analysis of the epicuticular wax layer on the surface of plant leaves can provide a unique window into plant physiology and responses to environmental stimuli. Well-established analytical methodologies can quantify epicuticular wax composition, yet few methods are capable of imaging wax distribution in situ or in vivo. Here, the first report of Fourier transform infrared (FTIR) reflectance spectroscopic imaging as a non-destructive, in situ, method to investigate variation in epicuticular wax distribution at 25 µm spatial resolution is presented. The authors demonstrate in vivo imaging of alterations in epicuticular waxes during leaf development and in situ imaging during plant disease or exposure to environmental stressors. It is envisaged that this new analytical capability will enable in vivo studies of plants to provide insights into how the physiology of plants and crops respond to environmental stresses such as disease, soil contamination, drought, soil acidity, and climate change. 2021 Journal Article http://hdl.handle.net/20.500.11937/90105 10.1002/advs.202101902 English http://purl.org/au-research/grants/arc/FT190100017 http://creativecommons.org/licenses/by/4.0/ WILEY fulltext |
| spellingShingle | Science & Technology Physical Sciences Technology Chemistry, Multidisciplinary Nanoscience & Nanotechnology Materials Science, Multidisciplinary Chemistry Science & Technology - Other Topics Materials Science epicuticular wax infrared microscopy plant physiology reflectance SPECTROSCOPY RAMAN STRESS ATR INTERFACE SURFACES Khambatta, Karina Hollings, Ashley Sauzier, Georgina Sanglard, Lilian M.V.P. Klein, A.R. Tobin, M.J. Vongsvivut, J. Gibberd, Mark Payne, Alan Naim, Fatima Hackett, Mark “Wax On, Wax Off”: In Vivo Imaging of Plant Physiology and Disease with Fourier Transform Infrared Reflectance Microspectroscopy |
| title | “Wax On, Wax Off”: In Vivo Imaging of Plant Physiology and Disease with Fourier Transform Infrared Reflectance Microspectroscopy |
| title_full | “Wax On, Wax Off”: In Vivo Imaging of Plant Physiology and Disease with Fourier Transform Infrared Reflectance Microspectroscopy |
| title_fullStr | “Wax On, Wax Off”: In Vivo Imaging of Plant Physiology and Disease with Fourier Transform Infrared Reflectance Microspectroscopy |
| title_full_unstemmed | “Wax On, Wax Off”: In Vivo Imaging of Plant Physiology and Disease with Fourier Transform Infrared Reflectance Microspectroscopy |
| title_short | “Wax On, Wax Off”: In Vivo Imaging of Plant Physiology and Disease with Fourier Transform Infrared Reflectance Microspectroscopy |
| title_sort | “wax on, wax off”: in vivo imaging of plant physiology and disease with fourier transform infrared reflectance microspectroscopy |
| topic | Science & Technology Physical Sciences Technology Chemistry, Multidisciplinary Nanoscience & Nanotechnology Materials Science, Multidisciplinary Chemistry Science & Technology - Other Topics Materials Science epicuticular wax infrared microscopy plant physiology reflectance SPECTROSCOPY RAMAN STRESS ATR INTERFACE SURFACES |
| url | http://purl.org/au-research/grants/arc/FT190100017 http://hdl.handle.net/20.500.11937/90105 |