In Vitro primary human airway epithelial whole exhaust exposure
The method outlined in this article is a customization of the whole exhaust exposure method generated by Mullins et al. (2016) using reprogrammed primary human airway epithelial cells as described by Martinovich et al. (2017). It has been used successfully to generate recently published data (Landwe...
| Main Authors: | , , , , , , , , |
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| Format: | Journal Article |
| Language: | English |
| Published: |
2021
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| Subjects: | |
| Online Access: | http://purl.org/au-research/grants/arc/DP170104346 http://hdl.handle.net/20.500.11937/90020 |
| _version_ | 1848765311757582336 |
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| author | Landwehr, Katherine R. Hillas, J. Mead-Hunter, Ryan Brooks, P. King, A. O'Leary, R.A. Kicic, Anthony Mullins, Ben Larcombe, Alexander |
| author_facet | Landwehr, Katherine R. Hillas, J. Mead-Hunter, Ryan Brooks, P. King, A. O'Leary, R.A. Kicic, Anthony Mullins, Ben Larcombe, Alexander |
| author_sort | Landwehr, Katherine R. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | The method outlined in this article is a customization of the whole exhaust exposure method generated by Mullins et al. (2016) using reprogrammed primary human airway epithelial cells as described by Martinovich et al. (2017). It has been used successfully to generate recently published data (Landwehr et al. 2021). The goal was to generate an exhaust exposure model where exhaust is collected from a modern engine, real-world exhaust concentrations are used and relevant tissues exposed to assess the effects of multiple biodiesel exposures. Exhaust was generated, gently vacuumed into a dilution chamber where it was diluted 1/15 with air and then vacuumed into an incubator containing the primary cell cultures for exposure. Exhaust physico-chemical properties including combustion gas concentrations and particle spectra were then analyzed using a combustion gas analyzer and a Universal Scanning Mobility Particle Sizer. 24 h after exposure, cellular viability and mediator release were measured using Annexin-V/PI staining and meditator multiplexing kits respectively. This method was generated to test biodiesel exhaust exposures but can be easily adapted for any type of engine exhaust exposure or even potentially other respirable environmental exposures such as woodsmoke. The main customization points for this method are: • Exhaust generated by a diesel engine equipped with EURO VI exhaust after treatment devices including diesel particulate filter and diesel oxidation catalyst. • The generated exhaust was diluted 1/15 with air to replicate real world exposure concentrations. • Used primary human airway epithelial cells obtained from bronchoscope brushings from multiple volunteers and reprogrammed to allow multiple, comparative exposures from the same individual. |
| first_indexed | 2025-11-14T11:33:14Z |
| format | Journal Article |
| id | curtin-20.500.11937-90020 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| language | eng |
| last_indexed | 2025-11-14T11:33:14Z |
| publishDate | 2021 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-900202023-02-07T07:15:59Z In Vitro primary human airway epithelial whole exhaust exposure Landwehr, Katherine R. Hillas, J. Mead-Hunter, Ryan Brooks, P. King, A. O'Leary, R.A. Kicic, Anthony Mullins, Ben Larcombe, Alexander Airway epithelial cells Biodiesel Environmental exposure Exhaust exposure Exposure protocol The method outlined in this article is a customization of the whole exhaust exposure method generated by Mullins et al. (2016) using reprogrammed primary human airway epithelial cells as described by Martinovich et al. (2017). It has been used successfully to generate recently published data (Landwehr et al. 2021). The goal was to generate an exhaust exposure model where exhaust is collected from a modern engine, real-world exhaust concentrations are used and relevant tissues exposed to assess the effects of multiple biodiesel exposures. Exhaust was generated, gently vacuumed into a dilution chamber where it was diluted 1/15 with air and then vacuumed into an incubator containing the primary cell cultures for exposure. Exhaust physico-chemical properties including combustion gas concentrations and particle spectra were then analyzed using a combustion gas analyzer and a Universal Scanning Mobility Particle Sizer. 24 h after exposure, cellular viability and mediator release were measured using Annexin-V/PI staining and meditator multiplexing kits respectively. This method was generated to test biodiesel exhaust exposures but can be easily adapted for any type of engine exhaust exposure or even potentially other respirable environmental exposures such as woodsmoke. The main customization points for this method are: • Exhaust generated by a diesel engine equipped with EURO VI exhaust after treatment devices including diesel particulate filter and diesel oxidation catalyst. • The generated exhaust was diluted 1/15 with air to replicate real world exposure concentrations. • Used primary human airway epithelial cells obtained from bronchoscope brushings from multiple volunteers and reprogrammed to allow multiple, comparative exposures from the same individual. 2021 Journal Article http://hdl.handle.net/20.500.11937/90020 10.1016/j.mex.2021.101561 eng http://purl.org/au-research/grants/arc/DP170104346 http://creativecommons.org/licenses/by-nc-nd/4.0/ fulltext |
| spellingShingle | Airway epithelial cells Biodiesel Environmental exposure Exhaust exposure Exposure protocol Landwehr, Katherine R. Hillas, J. Mead-Hunter, Ryan Brooks, P. King, A. O'Leary, R.A. Kicic, Anthony Mullins, Ben Larcombe, Alexander In Vitro primary human airway epithelial whole exhaust exposure |
| title | In Vitro primary human airway epithelial whole exhaust exposure |
| title_full | In Vitro primary human airway epithelial whole exhaust exposure |
| title_fullStr | In Vitro primary human airway epithelial whole exhaust exposure |
| title_full_unstemmed | In Vitro primary human airway epithelial whole exhaust exposure |
| title_short | In Vitro primary human airway epithelial whole exhaust exposure |
| title_sort | in vitro primary human airway epithelial whole exhaust exposure |
| topic | Airway epithelial cells Biodiesel Environmental exposure Exhaust exposure Exposure protocol |
| url | http://purl.org/au-research/grants/arc/DP170104346 http://hdl.handle.net/20.500.11937/90020 |