Fuel feedstock determines biodiesel exhaust toxicity in a human airway epithelial cell exposure model
Background: Biodiesel is promoted as a sustainable replacement for commercial diesel. Biodiesel fuel and exhaust properties change depending on the base feedstock oil/fat used during creation. The aims of this study were, for the first time, to compare the exhaust exposure health impacts of a wide r...
| Main Authors: | , , , , , , , , |
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| Format: | Journal Article |
| Language: | English |
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ELSEVIER
2021
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| Subjects: | |
| Online Access: | https://www.sciencedirect.com/science/article/am/pii/S0304389421016022 http://hdl.handle.net/20.500.11937/90019 |
| _version_ | 1848765311484952576 |
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| author | Landwehr, Katherine R. Hillas, J. Mead-Hunter, Ryan Brooks, P. King, Andrew O'Leary, R.A. Kicic, Anthony Mullins, Ben Larcombe, Alexander |
| author_facet | Landwehr, Katherine R. Hillas, J. Mead-Hunter, Ryan Brooks, P. King, Andrew O'Leary, R.A. Kicic, Anthony Mullins, Ben Larcombe, Alexander |
| author_sort | Landwehr, Katherine R. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | Background: Biodiesel is promoted as a sustainable replacement for commercial diesel. Biodiesel fuel and exhaust properties change depending on the base feedstock oil/fat used during creation. The aims of this study were, for the first time, to compare the exhaust exposure health impacts of a wide range of biodiesels made from different feedstocks and relate these effects with the corresponding exhaust characteristics. Method: Primary airway epithelial cells were exposed to diluted exhaust from an engine running on conventional diesel and biodiesel made from Soy, Canola, Waste Cooking Oil, Tallow, Palm and Cottonseed. Exhaust properties and cellular viability and mediator release were analysed post exposure. Results: The exhaust physico-chemistry of Tallow biodiesel was the most different to diesel as well as the most toxic, with exposure resulting in significantly decreased cellular viability (95.8 ± 6.5%) and increased release of several immune mediators including IL-6 (+223.11 ± 368.83 pg/mL) and IL-8 (+1516.17 ± 2908.79 pg/mL) above Air controls. In contrast Canola biodiesel was the least toxic with exposure only increasing TNF-α (4.91 ± 8.61). Conclusion: This study, which investigated the toxic effects for the largest range of biodiesels, shows that exposure to different exhausts results in a spectrum of toxic effects in vitro when combusted under identical conditions. |
| first_indexed | 2025-11-14T11:33:14Z |
| format | Journal Article |
| id | curtin-20.500.11937-90019 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T11:33:14Z |
| publishDate | 2021 |
| publisher | ELSEVIER |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-900192023-04-26T09:03:50Z Fuel feedstock determines biodiesel exhaust toxicity in a human airway epithelial cell exposure model Landwehr, Katherine R. Hillas, J. Mead-Hunter, Ryan Brooks, P. King, Andrew O'Leary, R.A. Kicic, Anthony Mullins, Ben Larcombe, Alexander Science & Technology Technology Life Sciences & Biomedicine Engineering, Environmental Environmental Sciences Engineering Environmental Sciences & Ecology Biodiesel Exhaust exposure Health In vitro exposure model Vehicle emissions DIESEL-ENGINE EXHAUST OXIDATIVE STRESS METHYL-ESTER PARTICULATE MATTER IN-VITRO CARDIOVASCULAR MORTALITY 2ND-GENERATION BIODIESEL KINEMATIC VISCOSITY INTERFERON-GAMMA SULFUR-CONTENT Background: Biodiesel is promoted as a sustainable replacement for commercial diesel. Biodiesel fuel and exhaust properties change depending on the base feedstock oil/fat used during creation. The aims of this study were, for the first time, to compare the exhaust exposure health impacts of a wide range of biodiesels made from different feedstocks and relate these effects with the corresponding exhaust characteristics. Method: Primary airway epithelial cells were exposed to diluted exhaust from an engine running on conventional diesel and biodiesel made from Soy, Canola, Waste Cooking Oil, Tallow, Palm and Cottonseed. Exhaust properties and cellular viability and mediator release were analysed post exposure. Results: The exhaust physico-chemistry of Tallow biodiesel was the most different to diesel as well as the most toxic, with exposure resulting in significantly decreased cellular viability (95.8 ± 6.5%) and increased release of several immune mediators including IL-6 (+223.11 ± 368.83 pg/mL) and IL-8 (+1516.17 ± 2908.79 pg/mL) above Air controls. In contrast Canola biodiesel was the least toxic with exposure only increasing TNF-α (4.91 ± 8.61). Conclusion: This study, which investigated the toxic effects for the largest range of biodiesels, shows that exposure to different exhausts results in a spectrum of toxic effects in vitro when combusted under identical conditions. 2021 Journal Article http://hdl.handle.net/20.500.11937/90019 10.1016/j.jhazmat.2021.126637 English https://www.sciencedirect.com/science/article/am/pii/S0304389421016022 http://purl.org/au-research/grants/arc/DP170104346 ELSEVIER unknown |
| spellingShingle | Science & Technology Technology Life Sciences & Biomedicine Engineering, Environmental Environmental Sciences Engineering Environmental Sciences & Ecology Biodiesel Exhaust exposure Health In vitro exposure model Vehicle emissions DIESEL-ENGINE EXHAUST OXIDATIVE STRESS METHYL-ESTER PARTICULATE MATTER IN-VITRO CARDIOVASCULAR MORTALITY 2ND-GENERATION BIODIESEL KINEMATIC VISCOSITY INTERFERON-GAMMA SULFUR-CONTENT Landwehr, Katherine R. Hillas, J. Mead-Hunter, Ryan Brooks, P. King, Andrew O'Leary, R.A. Kicic, Anthony Mullins, Ben Larcombe, Alexander Fuel feedstock determines biodiesel exhaust toxicity in a human airway epithelial cell exposure model |
| title | Fuel feedstock determines biodiesel exhaust toxicity in a human airway epithelial cell exposure model |
| title_full | Fuel feedstock determines biodiesel exhaust toxicity in a human airway epithelial cell exposure model |
| title_fullStr | Fuel feedstock determines biodiesel exhaust toxicity in a human airway epithelial cell exposure model |
| title_full_unstemmed | Fuel feedstock determines biodiesel exhaust toxicity in a human airway epithelial cell exposure model |
| title_short | Fuel feedstock determines biodiesel exhaust toxicity in a human airway epithelial cell exposure model |
| title_sort | fuel feedstock determines biodiesel exhaust toxicity in a human airway epithelial cell exposure model |
| topic | Science & Technology Technology Life Sciences & Biomedicine Engineering, Environmental Environmental Sciences Engineering Environmental Sciences & Ecology Biodiesel Exhaust exposure Health In vitro exposure model Vehicle emissions DIESEL-ENGINE EXHAUST OXIDATIVE STRESS METHYL-ESTER PARTICULATE MATTER IN-VITRO CARDIOVASCULAR MORTALITY 2ND-GENERATION BIODIESEL KINEMATIC VISCOSITY INTERFERON-GAMMA SULFUR-CONTENT |
| url | https://www.sciencedirect.com/science/article/am/pii/S0304389421016022 https://www.sciencedirect.com/science/article/am/pii/S0304389421016022 http://hdl.handle.net/20.500.11937/90019 |