The detection of CMV in saliva can mark a systemic infection with CMV in renal transplant recipients
Human cytomegalovirus (CMV) is often transmitted through saliva. The salivary gland is a site of CMV replication and saliva can be used to diagnose congenital CMV infections. CMV replication is monitored in whole blood or plasma in renal transplant recipients (RTR) and associates with clinical disea...
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| Format: | Journal Article |
| Language: | English |
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MDPI
2019
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| Online Access: | http://purl.org/au-research/grants/nhmrc/1068652 http://hdl.handle.net/20.500.11937/89471 |
| _version_ | 1848765227873599488 |
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| author | Waters, Shelley Lee, Silvia Lloyd, M. Irish, A. Price, Patricia |
| author_facet | Waters, Shelley Lee, Silvia Lloyd, M. Irish, A. Price, Patricia |
| author_sort | Waters, Shelley |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | Human cytomegalovirus (CMV) is often transmitted through saliva. The salivary gland is a site of CMV replication and saliva can be used to diagnose congenital CMV infections. CMV replication is monitored in whole blood or plasma in renal transplant recipients (RTR) and associates with clinical disease. However, these assays may not detect replication in the salivary gland and there is little data linking detection in saliva with systemic infection and clinical sequelae. RTR (n = 82) were recruited > 2 years after transplantation. An in-house quantitative PCR assay was used to detect CMV UL54 in saliva samples. CMV DNA was sought in plasma using a commercial assay. Vascular health was predicted using flow mediated dilatation (FMD) and plasma biomarkers. CMV-reactive antibodies were quantified by ELISA and circulating CMV-specific T-cells by an interferon-γ ELISpot assay. Vδ2− γδ T-cells were detected using multicolor flow cytometry reflecting population expansion after CMV infection. The presence of CMV DNA in saliva and plasma associated with plasma levels of antibodies reactive with CMV gB and with populations of circulating Vδ2− γδ T-cells (p < 0.01). T-cells reactive to CMV immediate early (IE)-1 protein were generally lower in patients with CMV DNA in saliva or plasma, but the level of significance varied (p = 0.02–0.16). Additionally, CMV DNA in saliva or plasma associated weakly with impaired FMD (p = 0.06–0.09). The data suggest that CMV detected in saliva reflects systemic infections in adult RTR. |
| first_indexed | 2025-11-14T11:31:54Z |
| format | Journal Article |
| id | curtin-20.500.11937-89471 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T11:31:54Z |
| publishDate | 2019 |
| publisher | MDPI |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-894712022-11-09T07:07:16Z The detection of CMV in saliva can mark a systemic infection with CMV in renal transplant recipients Waters, Shelley Lee, Silvia Lloyd, M. Irish, A. Price, Patricia Science & Technology Life Sciences & Biomedicine Physical Sciences Biochemistry & Molecular Biology Chemistry, Multidisciplinary Chemistry cytomegalovirus infection saliva renal transplantation CELL-ADHESION MOLECULE-1 GAMMA-DELTA CYTOMEGALOVIRUS ANTIBODY T-CELLS EXPRESSION RECOMBINANT SELECTIN PLASMA GB Human cytomegalovirus (CMV) is often transmitted through saliva. The salivary gland is a site of CMV replication and saliva can be used to diagnose congenital CMV infections. CMV replication is monitored in whole blood or plasma in renal transplant recipients (RTR) and associates with clinical disease. However, these assays may not detect replication in the salivary gland and there is little data linking detection in saliva with systemic infection and clinical sequelae. RTR (n = 82) were recruited > 2 years after transplantation. An in-house quantitative PCR assay was used to detect CMV UL54 in saliva samples. CMV DNA was sought in plasma using a commercial assay. Vascular health was predicted using flow mediated dilatation (FMD) and plasma biomarkers. CMV-reactive antibodies were quantified by ELISA and circulating CMV-specific T-cells by an interferon-γ ELISpot assay. Vδ2− γδ T-cells were detected using multicolor flow cytometry reflecting population expansion after CMV infection. The presence of CMV DNA in saliva and plasma associated with plasma levels of antibodies reactive with CMV gB and with populations of circulating Vδ2− γδ T-cells (p < 0.01). T-cells reactive to CMV immediate early (IE)-1 protein were generally lower in patients with CMV DNA in saliva or plasma, but the level of significance varied (p = 0.02–0.16). Additionally, CMV DNA in saliva or plasma associated weakly with impaired FMD (p = 0.06–0.09). The data suggest that CMV detected in saliva reflects systemic infections in adult RTR. 2019 Journal Article http://hdl.handle.net/20.500.11937/89471 10.3390/ijms20205230 English http://purl.org/au-research/grants/nhmrc/1068652 https://creativecommons.org/licenses/by/4.0/ MDPI fulltext |
| spellingShingle | Science & Technology Life Sciences & Biomedicine Physical Sciences Biochemistry & Molecular Biology Chemistry, Multidisciplinary Chemistry cytomegalovirus infection saliva renal transplantation CELL-ADHESION MOLECULE-1 GAMMA-DELTA CYTOMEGALOVIRUS ANTIBODY T-CELLS EXPRESSION RECOMBINANT SELECTIN PLASMA GB Waters, Shelley Lee, Silvia Lloyd, M. Irish, A. Price, Patricia The detection of CMV in saliva can mark a systemic infection with CMV in renal transplant recipients |
| title | The detection of CMV in saliva can mark a systemic infection with CMV in renal transplant recipients |
| title_full | The detection of CMV in saliva can mark a systemic infection with CMV in renal transplant recipients |
| title_fullStr | The detection of CMV in saliva can mark a systemic infection with CMV in renal transplant recipients |
| title_full_unstemmed | The detection of CMV in saliva can mark a systemic infection with CMV in renal transplant recipients |
| title_short | The detection of CMV in saliva can mark a systemic infection with CMV in renal transplant recipients |
| title_sort | detection of cmv in saliva can mark a systemic infection with cmv in renal transplant recipients |
| topic | Science & Technology Life Sciences & Biomedicine Physical Sciences Biochemistry & Molecular Biology Chemistry, Multidisciplinary Chemistry cytomegalovirus infection saliva renal transplantation CELL-ADHESION MOLECULE-1 GAMMA-DELTA CYTOMEGALOVIRUS ANTIBODY T-CELLS EXPRESSION RECOMBINANT SELECTIN PLASMA GB |
| url | http://purl.org/au-research/grants/nhmrc/1068652 http://hdl.handle.net/20.500.11937/89471 |