Human cytomegalovirus-encoded micrornas can be found in saliva samples from renal transplant recipients
Human cytomegalovirus (HCMV) infections are common following renal transplantation and may have long-lasting effects. HCMV can be measured directly by viral DNA or indirectly via host immune responses. HCMV-encoded microRNA (miRNA) may alter the pathobiology of HCMV infections and contribute to the...
| Main Authors: | , , , , , |
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| Format: | Journal Article |
| Language: | English |
| Published: |
2020
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| Subjects: | |
| Online Access: | http://purl.org/au-research/grants/nhmrc/1068652 http://hdl.handle.net/20.500.11937/89470 |
| _version_ | 1848765227611455488 |
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| author | Waters, Shelley Lee, Silvia Munyard, Kylie Irish, A. Price, Patricia Wang, B.H. |
| author_facet | Waters, Shelley Lee, Silvia Munyard, Kylie Irish, A. Price, Patricia Wang, B.H. |
| author_sort | Waters, Shelley |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | Human cytomegalovirus (HCMV) infections are common following renal transplantation and may have long-lasting effects. HCMV can be measured directly by viral DNA or indirectly via host immune responses. HCMV-encoded microRNA (miRNA) may alter the pathobiology of HCMV infections and contribute to the progression of HCMV disease. HCMV-encoded miRNAs can be detected in blood but have not been sought in saliva. We investigated saliva samples from 32 renal transplant recipients (RTR) and 12 seropositive healthy controls for whom immunological data was available. Five HCMV-encoded miRNAs (miR-UL112-5p, miR-US5-2-3p, miR-UL36, miR-US25-2-3p and miR-UL22A) were sought using primer probe assays. HCMV miRNA species were detected in saliva from 15 RTR and 3 healthy controls, with miR-US5-2-3p most commonly detected. The presence of HCMV miRNAs associated with increased T-cell responses to HCMV IE-1 in RTR, suggesting a link with frequent reactivations of HCMV. |
| first_indexed | 2025-11-14T11:31:54Z |
| format | Journal Article |
| id | curtin-20.500.11937-89470 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| language | eng |
| last_indexed | 2025-11-14T11:31:54Z |
| publishDate | 2020 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-894702022-11-09T07:20:55Z Human cytomegalovirus-encoded micrornas can be found in saliva samples from renal transplant recipients Waters, Shelley Lee, Silvia Munyard, Kylie Irish, A. Price, Patricia Wang, B.H. HCMV cytomegalovirus kidney transplant miRNA saliva Human cytomegalovirus (HCMV) infections are common following renal transplantation and may have long-lasting effects. HCMV can be measured directly by viral DNA or indirectly via host immune responses. HCMV-encoded microRNA (miRNA) may alter the pathobiology of HCMV infections and contribute to the progression of HCMV disease. HCMV-encoded miRNAs can be detected in blood but have not been sought in saliva. We investigated saliva samples from 32 renal transplant recipients (RTR) and 12 seropositive healthy controls for whom immunological data was available. Five HCMV-encoded miRNAs (miR-UL112-5p, miR-US5-2-3p, miR-UL36, miR-US25-2-3p and miR-UL22A) were sought using primer probe assays. HCMV miRNA species were detected in saliva from 15 RTR and 3 healthy controls, with miR-US5-2-3p most commonly detected. The presence of HCMV miRNAs associated with increased T-cell responses to HCMV IE-1 in RTR, suggesting a link with frequent reactivations of HCMV. 2020 Journal Article http://hdl.handle.net/20.500.11937/89470 10.3390/ncrna6040050 eng http://purl.org/au-research/grants/nhmrc/1068652 http://creativecommons.org/licenses/by/4.0/ fulltext |
| spellingShingle | HCMV cytomegalovirus kidney transplant miRNA saliva Waters, Shelley Lee, Silvia Munyard, Kylie Irish, A. Price, Patricia Wang, B.H. Human cytomegalovirus-encoded micrornas can be found in saliva samples from renal transplant recipients |
| title | Human cytomegalovirus-encoded micrornas can be found in saliva samples from renal transplant recipients |
| title_full | Human cytomegalovirus-encoded micrornas can be found in saliva samples from renal transplant recipients |
| title_fullStr | Human cytomegalovirus-encoded micrornas can be found in saliva samples from renal transplant recipients |
| title_full_unstemmed | Human cytomegalovirus-encoded micrornas can be found in saliva samples from renal transplant recipients |
| title_short | Human cytomegalovirus-encoded micrornas can be found in saliva samples from renal transplant recipients |
| title_sort | human cytomegalovirus-encoded micrornas can be found in saliva samples from renal transplant recipients |
| topic | HCMV cytomegalovirus kidney transplant miRNA saliva |
| url | http://purl.org/au-research/grants/nhmrc/1068652 http://hdl.handle.net/20.500.11937/89470 |