Reproductive Isolation Among Three Nocturnal Moth-Pollinated Sympatric Habenaria Species (Orchidaceae)

Comparison and quantification of multiple pre- and post-pollination barriers to interspecific hybridization are important to understand the factors promoting reproductive isolation. Such isolating factors have been studied recently in many flowering plant species which seek after the general roles a...

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Main Authors: Hai-Ping, Zhang, Zhi-Bin, Tao, Judith, Trunschke, Mani, Shrestha, Scaccabarozzi, Daniela, Hong, Wang, Zong-Xin, Ren
Format: Journal Article
Published: Frontiers Media 2022
Online Access:http://hdl.handle.net/20.500.11937/89444
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author Hai-Ping, Zhang
Zhi-Bin, Tao
Judith, Trunschke
Mani, Shrestha
Scaccabarozzi, Daniela
Hong, Wang
Zong-Xin, Ren
author_facet Hai-Ping, Zhang
Zhi-Bin, Tao
Judith, Trunschke
Mani, Shrestha
Scaccabarozzi, Daniela
Hong, Wang
Zong-Xin, Ren
author_sort Hai-Ping, Zhang
building Curtin Institutional Repository
collection Online Access
description Comparison and quantification of multiple pre- and post-pollination barriers to interspecific hybridization are important to understand the factors promoting reproductive isolation. Such isolating factors have been studied recently in many flowering plant species which seek after the general roles and relative strengths of different pre- and post-pollination barriers. In this study, we quantified six isolating factors (ecogeographic isolation, phenological isolation, pollinator isolation, pollinia-pistil interactions, fruit production, and seed development) that could possibly be acting as reproductive barriers at different stages among three sympatric Habenaria species (H. limprichtii, H. davidii, and H. delavayi). These three species overlap geographically but occupy different microhabitats varying in soil water content. They were isolated through pollinator interactions both ethologically (pollinator preference) and mechanically (pollinia attachment site), but to a variable degree for different species pairs. Interspecific crosses between H. limprichtii and H. davidii result in high fruit set, and embryo development suggested weak post-pollination barriers, whereas bidirectional crosses of H. delavayi with either of the other two species fail to produce fruits. Our results revealed that pollinators were the most important isolating barrier including both ethological and mechanical mechanisms, to maintain the boundaries among these three sympatric Habenaria species. Our study also highlights the importance of a combination of pre-and post-pollination barriers for species co-existence in Orchidaceae.
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spelling curtin-20.500.11937-894442022-11-09T06:32:11Z Reproductive Isolation Among Three Nocturnal Moth-Pollinated Sympatric Habenaria Species (Orchidaceae) Hai-Ping, Zhang Zhi-Bin, Tao Judith, Trunschke Mani, Shrestha Scaccabarozzi, Daniela Hong, Wang Zong-Xin, Ren Comparison and quantification of multiple pre- and post-pollination barriers to interspecific hybridization are important to understand the factors promoting reproductive isolation. Such isolating factors have been studied recently in many flowering plant species which seek after the general roles and relative strengths of different pre- and post-pollination barriers. In this study, we quantified six isolating factors (ecogeographic isolation, phenological isolation, pollinator isolation, pollinia-pistil interactions, fruit production, and seed development) that could possibly be acting as reproductive barriers at different stages among three sympatric Habenaria species (H. limprichtii, H. davidii, and H. delavayi). These three species overlap geographically but occupy different microhabitats varying in soil water content. They were isolated through pollinator interactions both ethologically (pollinator preference) and mechanically (pollinia attachment site), but to a variable degree for different species pairs. Interspecific crosses between H. limprichtii and H. davidii result in high fruit set, and embryo development suggested weak post-pollination barriers, whereas bidirectional crosses of H. delavayi with either of the other two species fail to produce fruits. Our results revealed that pollinators were the most important isolating barrier including both ethological and mechanical mechanisms, to maintain the boundaries among these three sympatric Habenaria species. Our study also highlights the importance of a combination of pre-and post-pollination barriers for species co-existence in Orchidaceae. 2022 Journal Article http://hdl.handle.net/20.500.11937/89444 10.3389/fpls.2022.908852 http://creativecommons.org/licenses/by/4.0/ Frontiers Media fulltext
spellingShingle Hai-Ping, Zhang
Zhi-Bin, Tao
Judith, Trunschke
Mani, Shrestha
Scaccabarozzi, Daniela
Hong, Wang
Zong-Xin, Ren
Reproductive Isolation Among Three Nocturnal Moth-Pollinated Sympatric Habenaria Species (Orchidaceae)
title Reproductive Isolation Among Three Nocturnal Moth-Pollinated Sympatric Habenaria Species (Orchidaceae)
title_full Reproductive Isolation Among Three Nocturnal Moth-Pollinated Sympatric Habenaria Species (Orchidaceae)
title_fullStr Reproductive Isolation Among Three Nocturnal Moth-Pollinated Sympatric Habenaria Species (Orchidaceae)
title_full_unstemmed Reproductive Isolation Among Three Nocturnal Moth-Pollinated Sympatric Habenaria Species (Orchidaceae)
title_short Reproductive Isolation Among Three Nocturnal Moth-Pollinated Sympatric Habenaria Species (Orchidaceae)
title_sort reproductive isolation among three nocturnal moth-pollinated sympatric habenaria species (orchidaceae)
url http://hdl.handle.net/20.500.11937/89444