In-vitro Evidence for Novel, Non-Classical, Alternative P450scc-Initiated Vitamin D Metabolic Pathways in Human Placenta via HPLC-UV/DAD and LC-ESI-MS/MS Analysis

Current findings identify and substantiate existence of previously unrecognised, likely, in-vivo alternative, non-classical P450scc-initiated vitamin D3/D2 metabolic pathways within in-vitro human placenta models. These pathways are further modified by CYP27A1 or CYP27B1 activity in human placenta a...

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Main Author: Shehabi, Haleem Ziad
Format: Thesis
Published: Curtin University 2020
Online Access:http://hdl.handle.net/20.500.11937/89121
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author Shehabi, Haleem Ziad
author_facet Shehabi, Haleem Ziad
author_sort Shehabi, Haleem Ziad
building Curtin Institutional Repository
collection Online Access
description Current findings identify and substantiate existence of previously unrecognised, likely, in-vivo alternative, non-classical P450scc-initiated vitamin D3/D2 metabolic pathways within in-vitro human placenta models. These pathways are further modified by CYP27A1 or CYP27B1 activity in human placenta and confirmed where required by purified human enzyme studies. This data was closely verified using equivalent in-vitro bovine adrenal cortex models and where necessary by purified bovine enzyme studies. Profiling was achieved by newly developed HPLC-UV/DAD and LC-ESI-MS/MS methodology.
first_indexed 2025-11-14T11:30:55Z
format Thesis
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institution Curtin University Malaysia
institution_category Local University
last_indexed 2025-11-14T11:30:55Z
publishDate 2020
publisher Curtin University
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spelling curtin-20.500.11937-891212022-08-08T01:33:47Z In-vitro Evidence for Novel, Non-Classical, Alternative P450scc-Initiated Vitamin D Metabolic Pathways in Human Placenta via HPLC-UV/DAD and LC-ESI-MS/MS Analysis Shehabi, Haleem Ziad Current findings identify and substantiate existence of previously unrecognised, likely, in-vivo alternative, non-classical P450scc-initiated vitamin D3/D2 metabolic pathways within in-vitro human placenta models. These pathways are further modified by CYP27A1 or CYP27B1 activity in human placenta and confirmed where required by purified human enzyme studies. This data was closely verified using equivalent in-vitro bovine adrenal cortex models and where necessary by purified bovine enzyme studies. Profiling was achieved by newly developed HPLC-UV/DAD and LC-ESI-MS/MS methodology. 2020 Thesis http://hdl.handle.net/20.500.11937/89121 Curtin University restricted
spellingShingle Shehabi, Haleem Ziad
In-vitro Evidence for Novel, Non-Classical, Alternative P450scc-Initiated Vitamin D Metabolic Pathways in Human Placenta via HPLC-UV/DAD and LC-ESI-MS/MS Analysis
title In-vitro Evidence for Novel, Non-Classical, Alternative P450scc-Initiated Vitamin D Metabolic Pathways in Human Placenta via HPLC-UV/DAD and LC-ESI-MS/MS Analysis
title_full In-vitro Evidence for Novel, Non-Classical, Alternative P450scc-Initiated Vitamin D Metabolic Pathways in Human Placenta via HPLC-UV/DAD and LC-ESI-MS/MS Analysis
title_fullStr In-vitro Evidence for Novel, Non-Classical, Alternative P450scc-Initiated Vitamin D Metabolic Pathways in Human Placenta via HPLC-UV/DAD and LC-ESI-MS/MS Analysis
title_full_unstemmed In-vitro Evidence for Novel, Non-Classical, Alternative P450scc-Initiated Vitamin D Metabolic Pathways in Human Placenta via HPLC-UV/DAD and LC-ESI-MS/MS Analysis
title_short In-vitro Evidence for Novel, Non-Classical, Alternative P450scc-Initiated Vitamin D Metabolic Pathways in Human Placenta via HPLC-UV/DAD and LC-ESI-MS/MS Analysis
title_sort in-vitro evidence for novel, non-classical, alternative p450scc-initiated vitamin d metabolic pathways in human placenta via hplc-uv/dad and lc-esi-ms/ms analysis
url http://hdl.handle.net/20.500.11937/89121