Influence of native ureolytic microbial community on biocementation potential of Sporosarcina pasteurii

Microbially induced calcium carbonate precipitation (MICP)/Biocementation has emerged as a promising technique for soil engineering applications. There are chiefly two methods by which MICP is applied for field applications including biostimulation and bioaugmentation. Although bioaugmentation strat...

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Main Authors: Murugan, Raja, Suraishkumar, G.K., Mukherjee, Abhijit, Dhami, Navdeep
Format: Journal Article
Language:English
Published: NATURE PORTFOLIO 2021
Subjects:
Online Access:http://purl.org/au-research/grants/arc/LP180100132
http://hdl.handle.net/20.500.11937/88268
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author Murugan, Raja
Suraishkumar, G.K.
Mukherjee, Abhijit
Dhami, Navdeep
author_facet Murugan, Raja
Suraishkumar, G.K.
Mukherjee, Abhijit
Dhami, Navdeep
author_sort Murugan, Raja
building Curtin Institutional Repository
collection Online Access
description Microbially induced calcium carbonate precipitation (MICP)/Biocementation has emerged as a promising technique for soil engineering applications. There are chiefly two methods by which MICP is applied for field applications including biostimulation and bioaugmentation. Although bioaugmentation strategy using efficient ureolytic biocementing culture of Sporosarcina pasteurii is widely practiced, the impact of native ureolytic microbial communities (NUMC) on CaCO3 mineralisation via S. pasteurii has not been explored. In this paper, we investigated the effect of different concentrations of NUMC on MICP kinetics and biomineral properties in the presence and absence of S. pasteurii. Kinetic analysis showed that the biocementation potential of S. pasteurii is sixfold higher than NUMC and is not significantly impacted even when the concentration of the NUMC is eight times higher. Micrographic results revealed a quick rate of CaCO3 precipitation by S. pasteurii leading to generation of smaller CaCO3 crystals (5–40 µm), while slow rate of CaCO3 precipitation by NUMC led to creation of larger CaCO3 crystals (35–100 µm). Mineralogical results showed the predominance of calcite phase in both sets. The outcome of current study is crucial for tailor-made applications of MICP.
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spelling curtin-20.500.11937-882682022-05-03T04:16:27Z Influence of native ureolytic microbial community on biocementation potential of Sporosarcina pasteurii Murugan, Raja Suraishkumar, G.K. Mukherjee, Abhijit Dhami, Navdeep Science & Technology Multidisciplinary Sciences Science & Technology - Other Topics INDUCED CARBONATE PRECIPITATION CALCITE PRECIPITATION BACILLUS-PASTEURII MORPHOLOGY KINETICS BACTERIA MINERALIZATION OPTIMIZATION TEMPERATURE IMPROVEMENT Microbially induced calcium carbonate precipitation (MICP)/Biocementation has emerged as a promising technique for soil engineering applications. There are chiefly two methods by which MICP is applied for field applications including biostimulation and bioaugmentation. Although bioaugmentation strategy using efficient ureolytic biocementing culture of Sporosarcina pasteurii is widely practiced, the impact of native ureolytic microbial communities (NUMC) on CaCO3 mineralisation via S. pasteurii has not been explored. In this paper, we investigated the effect of different concentrations of NUMC on MICP kinetics and biomineral properties in the presence and absence of S. pasteurii. Kinetic analysis showed that the biocementation potential of S. pasteurii is sixfold higher than NUMC and is not significantly impacted even when the concentration of the NUMC is eight times higher. Micrographic results revealed a quick rate of CaCO3 precipitation by S. pasteurii leading to generation of smaller CaCO3 crystals (5–40 µm), while slow rate of CaCO3 precipitation by NUMC led to creation of larger CaCO3 crystals (35–100 µm). Mineralogical results showed the predominance of calcite phase in both sets. The outcome of current study is crucial for tailor-made applications of MICP. 2021 Journal Article http://hdl.handle.net/20.500.11937/88268 10.1038/s41598-021-00315-5 English http://purl.org/au-research/grants/arc/LP180100132 http://creativecommons.org/licenses/by/4.0/ NATURE PORTFOLIO fulltext
spellingShingle Science & Technology
Multidisciplinary Sciences
Science & Technology - Other Topics
INDUCED CARBONATE PRECIPITATION
CALCITE PRECIPITATION
BACILLUS-PASTEURII
MORPHOLOGY
KINETICS
BACTERIA
MINERALIZATION
OPTIMIZATION
TEMPERATURE
IMPROVEMENT
Murugan, Raja
Suraishkumar, G.K.
Mukherjee, Abhijit
Dhami, Navdeep
Influence of native ureolytic microbial community on biocementation potential of Sporosarcina pasteurii
title Influence of native ureolytic microbial community on biocementation potential of Sporosarcina pasteurii
title_full Influence of native ureolytic microbial community on biocementation potential of Sporosarcina pasteurii
title_fullStr Influence of native ureolytic microbial community on biocementation potential of Sporosarcina pasteurii
title_full_unstemmed Influence of native ureolytic microbial community on biocementation potential of Sporosarcina pasteurii
title_short Influence of native ureolytic microbial community on biocementation potential of Sporosarcina pasteurii
title_sort influence of native ureolytic microbial community on biocementation potential of sporosarcina pasteurii
topic Science & Technology
Multidisciplinary Sciences
Science & Technology - Other Topics
INDUCED CARBONATE PRECIPITATION
CALCITE PRECIPITATION
BACILLUS-PASTEURII
MORPHOLOGY
KINETICS
BACTERIA
MINERALIZATION
OPTIMIZATION
TEMPERATURE
IMPROVEMENT
url http://purl.org/au-research/grants/arc/LP180100132
http://hdl.handle.net/20.500.11937/88268