Expansion and conservation of biosynthetic gene clusters in pathogenic Pyrenophora spp.
Pyrenophora is a fungal genus responsible for a number of major cereal diseases. Although fungi produce many specialised or secondary metabolites for defence and interacting with the surrounding environment, the repertoire of specialised metabolites (SM) within Pyrenophora pathogenic species remains...
| Main Authors: | , , , , , , |
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| Format: | Journal Article |
| Language: | English |
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MDPI
2020
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| Subjects: | |
| Online Access: | http://hdl.handle.net/20.500.11937/88122 |
| _version_ | 1848764966394396672 |
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| author | Moolhuijzen, Paula Muria Gonzalez, Jordi Syme, Robert Rawlinson, Catherine See, Pao Theen Moffat, Caroline Ellwood, Simon |
| author_facet | Moolhuijzen, Paula Muria Gonzalez, Jordi Syme, Robert Rawlinson, Catherine See, Pao Theen Moffat, Caroline Ellwood, Simon |
| author_sort | Moolhuijzen, Paula |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | Pyrenophora is a fungal genus responsible for a number of major cereal diseases. Although fungi produce many specialised or secondary metabolites for defence and interacting with the surrounding environment, the repertoire of specialised metabolites (SM) within Pyrenophora pathogenic species remains mostly uncharted. In this study, an in-depth comparative analysis of the P. teres f. teres, P teres f. maculata and P. tritici-repentis potential to produce SMs, based on in silico predicted biosynthetic gene clusters (BGCs), was conducted using genome assemblies from PacBio DNA reads. Conservation of BGCs between the Pyrenophora species included type I polyketide synthases, terpene synthases and the first reporting of a type III polyketide synthase in P teres f. maculata. P. teres isolates exhibited substantial expansion of non-ribosomal peptide synthases relative to P. tritici-repentis, hallmarked by the presence of tailoring cis-acting nitrogen methyltransferase domains. P. teres isolates also possessed unique non-ribosomal peptide synthase (NRPS)-indole and indole BGCs, while a P. tritici-repentis phytotoxin BGC for triticone production was absent in P. teres. These differences highlight diversification between the pathogens that reflects their different evolutionary histories, host adaption and lifestyles. |
| first_indexed | 2025-11-14T11:27:45Z |
| format | Journal Article |
| id | curtin-20.500.11937-88122 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T11:27:45Z |
| publishDate | 2020 |
| publisher | MDPI |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-881222022-03-28T01:05:39Z Expansion and conservation of biosynthetic gene clusters in pathogenic Pyrenophora spp. Moolhuijzen, Paula Muria Gonzalez, Jordi Syme, Robert Rawlinson, Catherine See, Pao Theen Moffat, Caroline Ellwood, Simon Science & Technology Life Sciences & Biomedicine Food Science & Technology Toxicology necrotrophic fungal pathogen synteny comparative genomics PKS NRPS secondary metabolism TERES F MACULATA TRITICI-REPENTIS NET BLOTCH BARLEY IDENTIFICATION METABOLITE VIRULENCE TOXIN WHEAT SPOT Pyrenophora is a fungal genus responsible for a number of major cereal diseases. Although fungi produce many specialised or secondary metabolites for defence and interacting with the surrounding environment, the repertoire of specialised metabolites (SM) within Pyrenophora pathogenic species remains mostly uncharted. In this study, an in-depth comparative analysis of the P. teres f. teres, P teres f. maculata and P. tritici-repentis potential to produce SMs, based on in silico predicted biosynthetic gene clusters (BGCs), was conducted using genome assemblies from PacBio DNA reads. Conservation of BGCs between the Pyrenophora species included type I polyketide synthases, terpene synthases and the first reporting of a type III polyketide synthase in P teres f. maculata. P. teres isolates exhibited substantial expansion of non-ribosomal peptide synthases relative to P. tritici-repentis, hallmarked by the presence of tailoring cis-acting nitrogen methyltransferase domains. P. teres isolates also possessed unique non-ribosomal peptide synthase (NRPS)-indole and indole BGCs, while a P. tritici-repentis phytotoxin BGC for triticone production was absent in P. teres. These differences highlight diversification between the pathogens that reflects their different evolutionary histories, host adaption and lifestyles. 2020 Journal Article http://hdl.handle.net/20.500.11937/88122 10.3390/toxins12040242 English http://creativecommons.org/licenses/by/4.0/ MDPI fulltext |
| spellingShingle | Science & Technology Life Sciences & Biomedicine Food Science & Technology Toxicology necrotrophic fungal pathogen synteny comparative genomics PKS NRPS secondary metabolism TERES F MACULATA TRITICI-REPENTIS NET BLOTCH BARLEY IDENTIFICATION METABOLITE VIRULENCE TOXIN WHEAT SPOT Moolhuijzen, Paula Muria Gonzalez, Jordi Syme, Robert Rawlinson, Catherine See, Pao Theen Moffat, Caroline Ellwood, Simon Expansion and conservation of biosynthetic gene clusters in pathogenic Pyrenophora spp. |
| title | Expansion and conservation of biosynthetic gene clusters in pathogenic Pyrenophora spp. |
| title_full | Expansion and conservation of biosynthetic gene clusters in pathogenic Pyrenophora spp. |
| title_fullStr | Expansion and conservation of biosynthetic gene clusters in pathogenic Pyrenophora spp. |
| title_full_unstemmed | Expansion and conservation of biosynthetic gene clusters in pathogenic Pyrenophora spp. |
| title_short | Expansion and conservation of biosynthetic gene clusters in pathogenic Pyrenophora spp. |
| title_sort | expansion and conservation of biosynthetic gene clusters in pathogenic pyrenophora spp. |
| topic | Science & Technology Life Sciences & Biomedicine Food Science & Technology Toxicology necrotrophic fungal pathogen synteny comparative genomics PKS NRPS secondary metabolism TERES F MACULATA TRITICI-REPENTIS NET BLOTCH BARLEY IDENTIFICATION METABOLITE VIRULENCE TOXIN WHEAT SPOT |
| url | http://hdl.handle.net/20.500.11937/88122 |