The identification and deletion of the polyketide synthase-nonribosomal peptide synthase gene responsible for the production of the phytotoxic triticone A/B in the wheat fungal pathogen Pyrenophora tritici-repentis
The economically important necrotrophic fungal pathogen, Pyrenophora tritici-repentis (Ptr), causes tan spot of wheat, a disease typified by foliar necrosis and chlorosis. The culture filtrate of an Australian Ptr isolate, M4, possesses phytotoxic activity and plant bioassay guided discovery led to...
| Main Authors: | , , , , , , |
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| Format: | Journal Article |
| Language: | English |
| Published: |
WILEY
2019
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| Online Access: | http://purl.org/au-research/grants/arc/FT160100233 http://hdl.handle.net/20.500.11937/88118 |
| _version_ | 1848764965202165760 |
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| author | Rawlinson, Catherine See, Pao Theen Moolhuijzen, Paula Li, H. Moffat, Caroline Chooi, Yit-Heng Oliver, Richard |
| author_facet | Rawlinson, Catherine See, Pao Theen Moolhuijzen, Paula Li, H. Moffat, Caroline Chooi, Yit-Heng Oliver, Richard |
| author_sort | Rawlinson, Catherine |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | The economically important necrotrophic fungal pathogen, Pyrenophora tritici-repentis (Ptr), causes tan spot of wheat, a disease typified by foliar necrosis and chlorosis. The culture filtrate of an Australian Ptr isolate, M4, possesses phytotoxic activity and plant bioassay guided discovery led to the purification of necrosis inducing toxins called triticone A and B. High-resolution LC–MS/MS analysis of the culture filtrate identified an additional 37 triticone-like compounds. The biosynthetic gene cluster responsible for triticone production (the Ttc cluster) was identified and deletion of TtcA, a hybrid polyketide synthase (PKS)-nonribosomal peptide synthase (NRPS), abolished production of all triticones. The pathogenicity of mutant (ttcA) strains was not visibly affected in our assays. We hypothesize that triticones possess general antimicrobial activity important for competition in multi-microbial environments. |
| first_indexed | 2025-11-14T11:27:44Z |
| format | Journal Article |
| id | curtin-20.500.11937-88118 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T11:27:44Z |
| publishDate | 2019 |
| publisher | WILEY |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-881182022-03-28T00:54:04Z The identification and deletion of the polyketide synthase-nonribosomal peptide synthase gene responsible for the production of the phytotoxic triticone A/B in the wheat fungal pathogen Pyrenophora tritici-repentis Rawlinson, Catherine See, Pao Theen Moolhuijzen, Paula Li, H. Moffat, Caroline Chooi, Yit-Heng Oliver, Richard Science & Technology Life Sciences & Biomedicine Microbiology TAN SPOT SECONDARY METABOLISM SPIROSTAPHYLOTRICHINS STRAIN CATENARIN CLUSTER LACTAM TOXA The economically important necrotrophic fungal pathogen, Pyrenophora tritici-repentis (Ptr), causes tan spot of wheat, a disease typified by foliar necrosis and chlorosis. The culture filtrate of an Australian Ptr isolate, M4, possesses phytotoxic activity and plant bioassay guided discovery led to the purification of necrosis inducing toxins called triticone A and B. High-resolution LC–MS/MS analysis of the culture filtrate identified an additional 37 triticone-like compounds. The biosynthetic gene cluster responsible for triticone production (the Ttc cluster) was identified and deletion of TtcA, a hybrid polyketide synthase (PKS)-nonribosomal peptide synthase (NRPS), abolished production of all triticones. The pathogenicity of mutant (ttcA) strains was not visibly affected in our assays. We hypothesize that triticones possess general antimicrobial activity important for competition in multi-microbial environments. 2019 Journal Article http://hdl.handle.net/20.500.11937/88118 10.1111/1462-2920.14854 English http://purl.org/au-research/grants/arc/FT160100233 http://creativecommons.org/licenses/by/4.0/ WILEY fulltext |
| spellingShingle | Science & Technology Life Sciences & Biomedicine Microbiology TAN SPOT SECONDARY METABOLISM SPIROSTAPHYLOTRICHINS STRAIN CATENARIN CLUSTER LACTAM TOXA Rawlinson, Catherine See, Pao Theen Moolhuijzen, Paula Li, H. Moffat, Caroline Chooi, Yit-Heng Oliver, Richard The identification and deletion of the polyketide synthase-nonribosomal peptide synthase gene responsible for the production of the phytotoxic triticone A/B in the wheat fungal pathogen Pyrenophora tritici-repentis |
| title | The identification and deletion of the polyketide synthase-nonribosomal peptide synthase gene responsible for the production of the phytotoxic triticone A/B in the wheat fungal pathogen Pyrenophora tritici-repentis |
| title_full | The identification and deletion of the polyketide synthase-nonribosomal peptide synthase gene responsible for the production of the phytotoxic triticone A/B in the wheat fungal pathogen Pyrenophora tritici-repentis |
| title_fullStr | The identification and deletion of the polyketide synthase-nonribosomal peptide synthase gene responsible for the production of the phytotoxic triticone A/B in the wheat fungal pathogen Pyrenophora tritici-repentis |
| title_full_unstemmed | The identification and deletion of the polyketide synthase-nonribosomal peptide synthase gene responsible for the production of the phytotoxic triticone A/B in the wheat fungal pathogen Pyrenophora tritici-repentis |
| title_short | The identification and deletion of the polyketide synthase-nonribosomal peptide synthase gene responsible for the production of the phytotoxic triticone A/B in the wheat fungal pathogen Pyrenophora tritici-repentis |
| title_sort | identification and deletion of the polyketide synthase-nonribosomal peptide synthase gene responsible for the production of the phytotoxic triticone a/b in the wheat fungal pathogen pyrenophora tritici-repentis |
| topic | Science & Technology Life Sciences & Biomedicine Microbiology TAN SPOT SECONDARY METABOLISM SPIROSTAPHYLOTRICHINS STRAIN CATENARIN CLUSTER LACTAM TOXA |
| url | http://purl.org/au-research/grants/arc/FT160100233 http://hdl.handle.net/20.500.11937/88118 |