Invertebrate DNA metabarcoding reveals changes in communities across mine site restoration chronosequences
Invertebrate biomonitoring can reveal crucial information about the status of restoration projects; however, it is routinely underused because of the high level of taxonomic expertise and resources required. Invertebrate DNA metabarcoding has been used to characterize invertebrate biodiversity but i...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Journal Article |
| Language: | English |
| Published: |
Wiley-Blackwell
2019
|
| Subjects: | |
| Online Access: | http://purl.org/au-research/grants/arc/IC150100041 http://hdl.handle.net/20.500.11937/87445 |
| _version_ | 1848764917213036544 |
|---|---|
| author | Fernandes, Kristen Van Der Heyde, Mieke Megan, Coghlan Wardell-Johnson, Grant Bunce, Michael Harris, Richard Nevill, Paul |
| author_facet | Fernandes, Kristen Van Der Heyde, Mieke Megan, Coghlan Wardell-Johnson, Grant Bunce, Michael Harris, Richard Nevill, Paul |
| author_sort | Fernandes, Kristen |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | Invertebrate biomonitoring can reveal crucial information about the status of restoration projects; however, it is routinely underused because of the high level of taxonomic expertise and resources required. Invertebrate DNA metabarcoding has been used to characterize invertebrate biodiversity but its application in restoration remains untested. We use DNA metabarcoding, a new approach for restoration assessment, to explore the invertebrate composition from pitfall traps at two mine site restoration chronosequences in southwestern Australia. Invertebrates were profiled using two cytochrome oxidase subunit 1 assays to investigate invertebrate biodiversity. The data revealed differences between invertebrate communities at the two mines and between the different age plots of the chronosequences. Several characteristic taxa were identified for each age within the chronosequence, including springtails within the youngest sites (Order: Collembola) and millipedes within the oldest and reference sites (Order: Julida). This study facilitates development of a molecular “toolkit” for the monitoring of ecological restoration projects. We suggest that a metabarcoding approach shows promise in complementing current monitoring practices that rely on alpha taxonomy. |
| first_indexed | 2025-11-14T11:26:58Z |
| format | Journal Article |
| id | curtin-20.500.11937-87445 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T11:26:58Z |
| publishDate | 2019 |
| publisher | Wiley-Blackwell |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-874452022-05-31T01:11:15Z Invertebrate DNA metabarcoding reveals changes in communities across mine site restoration chronosequences Fernandes, Kristen Van Der Heyde, Mieke Megan, Coghlan Wardell-Johnson, Grant Bunce, Michael Harris, Richard Nevill, Paul Science & Technology Life Sciences & Biomedicine Ecology Environmental Sciences & Ecology biomonitoring chronosequence metabarcoding mine site restoration next-generation sequencing Restoration Ecology FUNCTIONAL RECOVERY ECOSYSTEM SERVICES PLANT DIVERSITY ANTS SUCCESS BIOINDICATORS SEQUENCES RESPONSES RICHNESS Invertebrate biomonitoring can reveal crucial information about the status of restoration projects; however, it is routinely underused because of the high level of taxonomic expertise and resources required. Invertebrate DNA metabarcoding has been used to characterize invertebrate biodiversity but its application in restoration remains untested. We use DNA metabarcoding, a new approach for restoration assessment, to explore the invertebrate composition from pitfall traps at two mine site restoration chronosequences in southwestern Australia. Invertebrates were profiled using two cytochrome oxidase subunit 1 assays to investigate invertebrate biodiversity. The data revealed differences between invertebrate communities at the two mines and between the different age plots of the chronosequences. Several characteristic taxa were identified for each age within the chronosequence, including springtails within the youngest sites (Order: Collembola) and millipedes within the oldest and reference sites (Order: Julida). This study facilitates development of a molecular “toolkit” for the monitoring of ecological restoration projects. We suggest that a metabarcoding approach shows promise in complementing current monitoring practices that rely on alpha taxonomy. 2019 Journal Article http://hdl.handle.net/20.500.11937/87445 10.1111/rec.12976 English http://purl.org/au-research/grants/arc/IC150100041 Wiley-Blackwell fulltext |
| spellingShingle | Science & Technology Life Sciences & Biomedicine Ecology Environmental Sciences & Ecology biomonitoring chronosequence metabarcoding mine site restoration next-generation sequencing Restoration Ecology FUNCTIONAL RECOVERY ECOSYSTEM SERVICES PLANT DIVERSITY ANTS SUCCESS BIOINDICATORS SEQUENCES RESPONSES RICHNESS Fernandes, Kristen Van Der Heyde, Mieke Megan, Coghlan Wardell-Johnson, Grant Bunce, Michael Harris, Richard Nevill, Paul Invertebrate DNA metabarcoding reveals changes in communities across mine site restoration chronosequences |
| title | Invertebrate DNA metabarcoding reveals changes in communities across mine site restoration chronosequences |
| title_full | Invertebrate DNA metabarcoding reveals changes in communities across mine site restoration chronosequences |
| title_fullStr | Invertebrate DNA metabarcoding reveals changes in communities across mine site restoration chronosequences |
| title_full_unstemmed | Invertebrate DNA metabarcoding reveals changes in communities across mine site restoration chronosequences |
| title_short | Invertebrate DNA metabarcoding reveals changes in communities across mine site restoration chronosequences |
| title_sort | invertebrate dna metabarcoding reveals changes in communities across mine site restoration chronosequences |
| topic | Science & Technology Life Sciences & Biomedicine Ecology Environmental Sciences & Ecology biomonitoring chronosequence metabarcoding mine site restoration next-generation sequencing Restoration Ecology FUNCTIONAL RECOVERY ECOSYSTEM SERVICES PLANT DIVERSITY ANTS SUCCESS BIOINDICATORS SEQUENCES RESPONSES RICHNESS |
| url | http://purl.org/au-research/grants/arc/IC150100041 http://hdl.handle.net/20.500.11937/87445 |