Haliphthoros milfordensis isolated from eggs and larvae of mud crab (Scylla tranquebarica) in Sabah, Malaysia
Fungal infection occurred during mud crab spawning in a hatchery and caused almost 100% mortality in mud crab larvae after 5 days post-hatching. A fungus was isolated from eggs and larvae using PYGS agar and named IPMB 1603. During morphological identification, the strain IPMB 1603 was observed to p...
| Main Authors: | , , |
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| Format: | Journal Article |
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2017
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| Online Access: | https://eafp.org/download/2017-volume37/issue_6/37-6-226-hatai.pdf http://hdl.handle.net/20.500.11937/84708 |
| _version_ | 1848764677240127488 |
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| author | Lee, Yih Nin Hatai, K. Kurata, O. |
| author_facet | Lee, Yih Nin Hatai, K. Kurata, O. |
| author_sort | Lee, Yih Nin |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | Fungal infection occurred during mud crab spawning in a hatchery and caused almost 100% mortality in mud crab larvae after 5 days post-hatching. A fungus was isolated from eggs and larvae using PYGS agar and named IPMB 1603. During morphological identification, the strain IPMB 1603 was observed to produce a fragment inside the hyphae and the zoospores swam away from the tips of the discharge tube on several occasions, this closely resembling the genus Haliphthoros. Following comparisons made on the nucleotide sequence of the ITS1 region, the strain IPMB 1603 was identified belonging to a cluster of Haliphthoros milfordensis sharing 97-100% similarity. In this study, the strain IPMB 1603 was found to be an euryhaline fungus as the strain was able to grow on PYG agar containing NaCl. Strain IPMB 1603 only grew at certain salinity ranges, in which on PYGS agar contained sea water higher than 10 ppt. The optimum temperature range for fungal growth of strain IPMB 1603 was 30-35cC. Different growth rates were demonstrated at pH ranging from 4 to 9, but optimum growth was observed at pH 6 to 8. This is the first record of Haliphthoros milfordensis infection in Malaysia. |
| first_indexed | 2025-11-14T11:23:09Z |
| format | Journal Article |
| id | curtin-20.500.11937-84708 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T11:23:09Z |
| publishDate | 2017 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-847082021-08-11T00:41:40Z Haliphthoros milfordensis isolated from eggs and larvae of mud crab (Scylla tranquebarica) in Sabah, Malaysia Lee, Yih Nin Hatai, K. Kurata, O. Fungal infection occurred during mud crab spawning in a hatchery and caused almost 100% mortality in mud crab larvae after 5 days post-hatching. A fungus was isolated from eggs and larvae using PYGS agar and named IPMB 1603. During morphological identification, the strain IPMB 1603 was observed to produce a fragment inside the hyphae and the zoospores swam away from the tips of the discharge tube on several occasions, this closely resembling the genus Haliphthoros. Following comparisons made on the nucleotide sequence of the ITS1 region, the strain IPMB 1603 was identified belonging to a cluster of Haliphthoros milfordensis sharing 97-100% similarity. In this study, the strain IPMB 1603 was found to be an euryhaline fungus as the strain was able to grow on PYG agar containing NaCl. Strain IPMB 1603 only grew at certain salinity ranges, in which on PYGS agar contained sea water higher than 10 ppt. The optimum temperature range for fungal growth of strain IPMB 1603 was 30-35cC. Different growth rates were demonstrated at pH ranging from 4 to 9, but optimum growth was observed at pH 6 to 8. This is the first record of Haliphthoros milfordensis infection in Malaysia. 2017 Journal Article http://hdl.handle.net/20.500.11937/84708 https://eafp.org/download/2017-volume37/issue_6/37-6-226-hatai.pdf http://creativecommons.org/licenses/by/2.5/scotland/ fulltext |
| spellingShingle | Lee, Yih Nin Hatai, K. Kurata, O. Haliphthoros milfordensis isolated from eggs and larvae of mud crab (Scylla tranquebarica) in Sabah, Malaysia |
| title | Haliphthoros milfordensis isolated from eggs and larvae of mud crab (Scylla tranquebarica) in Sabah, Malaysia |
| title_full | Haliphthoros milfordensis isolated from eggs and larvae of mud crab (Scylla tranquebarica) in Sabah, Malaysia |
| title_fullStr | Haliphthoros milfordensis isolated from eggs and larvae of mud crab (Scylla tranquebarica) in Sabah, Malaysia |
| title_full_unstemmed | Haliphthoros milfordensis isolated from eggs and larvae of mud crab (Scylla tranquebarica) in Sabah, Malaysia |
| title_short | Haliphthoros milfordensis isolated from eggs and larvae of mud crab (Scylla tranquebarica) in Sabah, Malaysia |
| title_sort | haliphthoros milfordensis isolated from eggs and larvae of mud crab (scylla tranquebarica) in sabah, malaysia |
| url | https://eafp.org/download/2017-volume37/issue_6/37-6-226-hatai.pdf http://hdl.handle.net/20.500.11937/84708 |