Embryo culture: Can we perform better than nature?

Culture of preimplantation-stage embryos has always been a key element of laboratory embryology and has contributed substantially to the success of many assisted reproduction procedures. During the past decade, its importance has increased as extended in-vitro embryo culture and single blastocyst tr...

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Main Authors: Vajta, G., Rienzi, L., Cobo, A., Yovich, John
Format: Journal Article
Published: Elsevier Ltd 2010
Online Access:http://hdl.handle.net/20.500.11937/8253
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author Vajta, G.
Rienzi, L.
Cobo, A.
Yovich, John
author_facet Vajta, G.
Rienzi, L.
Cobo, A.
Yovich, John
author_sort Vajta, G.
building Curtin Institutional Repository
collection Online Access
description Culture of preimplantation-stage embryos has always been a key element of laboratory embryology and has contributed substantially to the success of many assisted reproduction procedures. During the past decade, its importance has increased as extended in-vitro embryo culture and single blastocyst transfer have become indispensable parts of the approach to decreasing the chance of multiple pregnancy while preserving the overall efficiency of the treatment. However, in spite of the scientific and commercial challenge stimulating research worldwide to optimize embryo culture conditions, a consensus is missing even in the basic principles, including composition and exchange of media, the required physical and biological environment and even the temperature of incubation. This review attempts to summarize the controversies, demonstrate the fragility of some widely accepted dogmas and generate an open-minded debate towards rapid and efficient optimization. New approaches expanding the traditional frames of mammalian embryo culture are also discussed. Although some researchers suppose that the efficiency of the presently applied in-vitro culture systems have already approached the biological limits, authors are confident that substantial improvement may be achieved that may expand considerably the possibilities of future assisted reproduction in humans. © 2010, Reproductive Healthcare Ltd. Published by Elsevier Ltd. All rights reserved.
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spelling curtin-20.500.11937-82532017-09-13T14:37:01Z Embryo culture: Can we perform better than nature? Vajta, G. Rienzi, L. Cobo, A. Yovich, John Culture of preimplantation-stage embryos has always been a key element of laboratory embryology and has contributed substantially to the success of many assisted reproduction procedures. During the past decade, its importance has increased as extended in-vitro embryo culture and single blastocyst transfer have become indispensable parts of the approach to decreasing the chance of multiple pregnancy while preserving the overall efficiency of the treatment. However, in spite of the scientific and commercial challenge stimulating research worldwide to optimize embryo culture conditions, a consensus is missing even in the basic principles, including composition and exchange of media, the required physical and biological environment and even the temperature of incubation. This review attempts to summarize the controversies, demonstrate the fragility of some widely accepted dogmas and generate an open-minded debate towards rapid and efficient optimization. New approaches expanding the traditional frames of mammalian embryo culture are also discussed. Although some researchers suppose that the efficiency of the presently applied in-vitro culture systems have already approached the biological limits, authors are confident that substantial improvement may be achieved that may expand considerably the possibilities of future assisted reproduction in humans. © 2010, Reproductive Healthcare Ltd. Published by Elsevier Ltd. All rights reserved. 2010 Journal Article http://hdl.handle.net/20.500.11937/8253 10.1016/j.rbmo.2009.12.018 Elsevier Ltd restricted
spellingShingle Vajta, G.
Rienzi, L.
Cobo, A.
Yovich, John
Embryo culture: Can we perform better than nature?
title Embryo culture: Can we perform better than nature?
title_full Embryo culture: Can we perform better than nature?
title_fullStr Embryo culture: Can we perform better than nature?
title_full_unstemmed Embryo culture: Can we perform better than nature?
title_short Embryo culture: Can we perform better than nature?
title_sort embryo culture: can we perform better than nature?
url http://hdl.handle.net/20.500.11937/8253