Electroimmunoblotting of myelin basic protein peptides: a novel approach to the rapid characterisation of antigenic specificities of monoclonal and polyclonal anti-MBP antibodies
A rapid and sensitive method for the identification of antigenic determinants recognised by monoclonal and polyclonal antibodies directed against myelin basic protein (MBP) is described. By electroimmunoblotting a series of overlapping peptides covering the entire MBP molecule with monoclonal anti-M...
| Main Authors: | , , , , , |
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| Format: | Journal Article |
| Language: | English |
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Elsevier
1988
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| Subjects: | |
| Online Access: | http://hdl.handle.net/20.500.11937/81499 |
| _version_ | 1848764379068104704 |
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| author | Sheng, H.Z. Martenson, R.E. Grgacic, E.V. Dowse, Carol Carnegie, R.L. Bernard, C.C.A. |
| author_facet | Sheng, H.Z. Martenson, R.E. Grgacic, E.V. Dowse, Carol Carnegie, R.L. Bernard, C.C.A. |
| author_sort | Sheng, H.Z. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | A rapid and sensitive method for the identification of antigenic determinants recognised by monoclonal and polyclonal antibodies directed against myelin basic protein (MBP) is described. By electroimmunoblotting a series of overlapping peptides covering the entire MBP molecule with monoclonal anti-MBP antibodies, the binding pattern of immunoreactive peptides can be rapidly determined and the reactive antigenic determinant identified. This procedure, which can be performed with both native and synthetic peptides, can also with appropriate modification, be applied to the analysis of naturally occuring or experimentally induced polyclonal anti-MBP autoantibodies. © 1988. |
| first_indexed | 2025-11-14T11:18:25Z |
| format | Journal Article |
| id | curtin-20.500.11937-81499 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T11:18:25Z |
| publishDate | 1988 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-814992020-11-03T04:55:07Z Electroimmunoblotting of myelin basic protein peptides: a novel approach to the rapid characterisation of antigenic specificities of monoclonal and polyclonal anti-MBP antibodies Sheng, H.Z. Martenson, R.E. Grgacic, E.V. Dowse, Carol Carnegie, R.L. Bernard, C.C.A. Science & Technology Life Sciences & Biomedicine Immunology Neurosciences Neurosciences & Neurology A rapid and sensitive method for the identification of antigenic determinants recognised by monoclonal and polyclonal antibodies directed against myelin basic protein (MBP) is described. By electroimmunoblotting a series of overlapping peptides covering the entire MBP molecule with monoclonal anti-MBP antibodies, the binding pattern of immunoreactive peptides can be rapidly determined and the reactive antigenic determinant identified. This procedure, which can be performed with both native and synthetic peptides, can also with appropriate modification, be applied to the analysis of naturally occuring or experimentally induced polyclonal anti-MBP autoantibodies. © 1988. 1988 Journal Article http://hdl.handle.net/20.500.11937/81499 10.1016/0165-5728(88)90066-5 English Elsevier restricted |
| spellingShingle | Science & Technology Life Sciences & Biomedicine Immunology Neurosciences Neurosciences & Neurology Sheng, H.Z. Martenson, R.E. Grgacic, E.V. Dowse, Carol Carnegie, R.L. Bernard, C.C.A. Electroimmunoblotting of myelin basic protein peptides: a novel approach to the rapid characterisation of antigenic specificities of monoclonal and polyclonal anti-MBP antibodies |
| title | Electroimmunoblotting of myelin basic protein peptides: a novel approach to the rapid characterisation of antigenic specificities of monoclonal and polyclonal anti-MBP antibodies |
| title_full | Electroimmunoblotting of myelin basic protein peptides: a novel approach to the rapid characterisation of antigenic specificities of monoclonal and polyclonal anti-MBP antibodies |
| title_fullStr | Electroimmunoblotting of myelin basic protein peptides: a novel approach to the rapid characterisation of antigenic specificities of monoclonal and polyclonal anti-MBP antibodies |
| title_full_unstemmed | Electroimmunoblotting of myelin basic protein peptides: a novel approach to the rapid characterisation of antigenic specificities of monoclonal and polyclonal anti-MBP antibodies |
| title_short | Electroimmunoblotting of myelin basic protein peptides: a novel approach to the rapid characterisation of antigenic specificities of monoclonal and polyclonal anti-MBP antibodies |
| title_sort | electroimmunoblotting of myelin basic protein peptides: a novel approach to the rapid characterisation of antigenic specificities of monoclonal and polyclonal anti-mbp antibodies |
| topic | Science & Technology Life Sciences & Biomedicine Immunology Neurosciences Neurosciences & Neurology |
| url | http://hdl.handle.net/20.500.11937/81499 |