An analysis of the feasibility of short read sequencing.
Several methods for ultra high-throughput DNA sequencing are currently under investigation. Many of these methods yield very short blocks of sequence information (reads). Here we report on an analysis showing the level of genome sequencing possible as a function of read length. It is shown that re-s...
| Main Authors: | , , , , , , , |
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| Format: | Journal Article |
| Language: | English |
| Published: |
Oxford University Press
2005
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| Subjects: | |
| Online Access: | http://hdl.handle.net/20.500.11937/81475 |
| _version_ | 1848764372683325440 |
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| author | Whiteford, N. Haslam, N. Weber, G. Prügel-Bennett, A. Essex, J.W. Roach, P.L. Bradley, M. Neylon, Cameron |
| author_facet | Whiteford, N. Haslam, N. Weber, G. Prügel-Bennett, A. Essex, J.W. Roach, P.L. Bradley, M. Neylon, Cameron |
| author_sort | Whiteford, N. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | Several methods for ultra high-throughput DNA sequencing are currently under investigation. Many of these methods yield very short blocks of sequence information (reads). Here we report on an analysis showing the level of genome sequencing possible as a function of read length. It is shown that re-sequencing and de novo sequencing of the majority of a bacterial genome is possible with read lengths of 20-30 nt, and that reads of 50 nt can provide reconstructed contigs (a contiguous fragment of sequence data) of 1000 nt and greater that cover 80% of human chromosome 1. |
| first_indexed | 2025-11-14T11:18:19Z |
| format | Journal Article |
| id | curtin-20.500.11937-81475 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| language | eng |
| last_indexed | 2025-11-14T11:18:19Z |
| publishDate | 2005 |
| publisher | Oxford University Press |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-814752020-11-09T03:42:13Z An analysis of the feasibility of short read sequencing. Whiteford, N. Haslam, N. Weber, G. Prügel-Bennett, A. Essex, J.W. Roach, P.L. Bradley, M. Neylon, Cameron Chromosomes, Human, Pair 1 Feasibility Studies Genome, Bacterial Genome, Human Genome, Viral Genomics Humans Sequence Analysis, DNA Several methods for ultra high-throughput DNA sequencing are currently under investigation. Many of these methods yield very short blocks of sequence information (reads). Here we report on an analysis showing the level of genome sequencing possible as a function of read length. It is shown that re-sequencing and de novo sequencing of the majority of a bacterial genome is possible with read lengths of 20-30 nt, and that reads of 50 nt can provide reconstructed contigs (a contiguous fragment of sequence data) of 1000 nt and greater that cover 80% of human chromosome 1. 2005 Journal Article http://hdl.handle.net/20.500.11937/81475 10.1093/nar/gni170 eng http://creativecommons.org/licenses/by-nc/4.0/ Oxford University Press fulltext |
| spellingShingle | Chromosomes, Human, Pair 1 Feasibility Studies Genome, Bacterial Genome, Human Genome, Viral Genomics Humans Sequence Analysis, DNA Whiteford, N. Haslam, N. Weber, G. Prügel-Bennett, A. Essex, J.W. Roach, P.L. Bradley, M. Neylon, Cameron An analysis of the feasibility of short read sequencing. |
| title | An analysis of the feasibility of short read sequencing. |
| title_full | An analysis of the feasibility of short read sequencing. |
| title_fullStr | An analysis of the feasibility of short read sequencing. |
| title_full_unstemmed | An analysis of the feasibility of short read sequencing. |
| title_short | An analysis of the feasibility of short read sequencing. |
| title_sort | analysis of the feasibility of short read sequencing. |
| topic | Chromosomes, Human, Pair 1 Feasibility Studies Genome, Bacterial Genome, Human Genome, Viral Genomics Humans Sequence Analysis, DNA |
| url | http://hdl.handle.net/20.500.11937/81475 |