The design, synthesis and biological evaluation of novel URB602 analogues as potential monoacylglycerol lipase inhibitors

We have synthesised an extensive series of URB602 analogues as inhibitors of monoacylglycerol lipase (MAGL), which is the major enzyme responsible for metabolising the endocannabinoid 2-arachidonylglycerol. The recently identified crystal structure of MAGL was used in the design strategy and reveale...

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Main Authors: Szabo, M., Agostino, Mark, Malone, D., Yuriev, E., Capuano, B.
Format: Journal Article
Published: Pergamon 2011
Subjects:
Online Access:http://hdl.handle.net/20.500.11937/8137
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author Szabo, M.
Agostino, Mark
Malone, D.
Yuriev, E.
Capuano, B.
author_facet Szabo, M.
Agostino, Mark
Malone, D.
Yuriev, E.
Capuano, B.
author_sort Szabo, M.
building Curtin Institutional Repository
collection Online Access
description We have synthesised an extensive series of URB602 analogues as inhibitors of monoacylglycerol lipase (MAGL), which is the major enzyme responsible for metabolising the endocannabinoid 2-arachidonylglycerol. The recently identified crystal structure of MAGL was used in the design strategy and revealed three possible binding sites for URB602 and the proposed analogues. A test series of carbamate analogues were docked into the identified sites to predict the most favourable binding location. The synthesised analogues of URB602 explored the biological effects of isosteric replacement, ring size and substitution, para substitution of the biphenyl moiety and the incorporation of a bicyclic element. The compounds were tested for their ability to inhibit human MAGL. The carbamate analogue 16 displayed the most significant inhibitory activity, reducing MAGL activity to 26% of controls at 100 μM compared to 73% for the parent compound URB602.
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institution Curtin University Malaysia
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publishDate 2011
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spelling curtin-20.500.11937-81372017-09-13T14:35:41Z The design, synthesis and biological evaluation of novel URB602 analogues as potential monoacylglycerol lipase inhibitors Szabo, M. Agostino, Mark Malone, D. Yuriev, E. Capuano, B. Monoacylglycerol lipase Endocannabinoid system Structure–activity relationship 2-Arachidonylglycerol URB602 We have synthesised an extensive series of URB602 analogues as inhibitors of monoacylglycerol lipase (MAGL), which is the major enzyme responsible for metabolising the endocannabinoid 2-arachidonylglycerol. The recently identified crystal structure of MAGL was used in the design strategy and revealed three possible binding sites for URB602 and the proposed analogues. A test series of carbamate analogues were docked into the identified sites to predict the most favourable binding location. The synthesised analogues of URB602 explored the biological effects of isosteric replacement, ring size and substitution, para substitution of the biphenyl moiety and the incorporation of a bicyclic element. The compounds were tested for their ability to inhibit human MAGL. The carbamate analogue 16 displayed the most significant inhibitory activity, reducing MAGL activity to 26% of controls at 100 μM compared to 73% for the parent compound URB602. 2011 Journal Article http://hdl.handle.net/20.500.11937/8137 10.1016/j.bmcl.2011.09.038 Pergamon restricted
spellingShingle Monoacylglycerol lipase
Endocannabinoid system
Structure–activity relationship
2-Arachidonylglycerol
URB602
Szabo, M.
Agostino, Mark
Malone, D.
Yuriev, E.
Capuano, B.
The design, synthesis and biological evaluation of novel URB602 analogues as potential monoacylglycerol lipase inhibitors
title The design, synthesis and biological evaluation of novel URB602 analogues as potential monoacylglycerol lipase inhibitors
title_full The design, synthesis and biological evaluation of novel URB602 analogues as potential monoacylglycerol lipase inhibitors
title_fullStr The design, synthesis and biological evaluation of novel URB602 analogues as potential monoacylglycerol lipase inhibitors
title_full_unstemmed The design, synthesis and biological evaluation of novel URB602 analogues as potential monoacylglycerol lipase inhibitors
title_short The design, synthesis and biological evaluation of novel URB602 analogues as potential monoacylglycerol lipase inhibitors
title_sort design, synthesis and biological evaluation of novel urb602 analogues as potential monoacylglycerol lipase inhibitors
topic Monoacylglycerol lipase
Endocannabinoid system
Structure–activity relationship
2-Arachidonylglycerol
URB602
url http://hdl.handle.net/20.500.11937/8137