A DNA barcoding approach to identify plant species in multiflower honey

The purpose of this study was to test the ability of DNA barcoding to identify the plant origins of processed honey. Four multifloral honeys produced at different sites in a floristically rich area in the northern Italian Alps were examined by using the rbcL and trnH-psbA plastid regions as barcode...

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Main Authors: Caridi, L., Bruni, I., Galimberti, A., Scaccabarozzi, Daniela, De Mattia, F., Casiraghi, M., Labra, M.
Format: Journal Article
Published: Elsevier 2015
Online Access:http://hdl.handle.net/20.500.11937/81260
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author Caridi, L.
Bruni, I.
Galimberti, A.
Scaccabarozzi, Daniela
De Mattia, F.
Casiraghi, M.
Labra, M.
author_facet Caridi, L.
Bruni, I.
Galimberti, A.
Scaccabarozzi, Daniela
De Mattia, F.
Casiraghi, M.
Labra, M.
author_sort Caridi, L.
building Curtin Institutional Repository
collection Online Access
description The purpose of this study was to test the ability of DNA barcoding to identify the plant origins of processed honey. Four multifloral honeys produced at different sites in a floristically rich area in the northern Italian Alps were examined by using the rbcL and trnH-psbA plastid regions as barcode markers. An extensive reference database of barcode sequences was generated for the local flora to determine the taxonomic composition of honey. Thirty-nine plant species were identified in the four honey samples, each of which originated from a mix of common plants belonging to Castanea, Quercus, Fagus and several herbaceous taxa. Interestingly, at least one endemic plant was found in all four honey samples, providing a clear signature for the geographic identity of these products. DNA of the toxic plant Atropa belladonna was detected in one sample, illustrating the usefulness of DNA barcoding for evaluating the safety of honey.
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format Journal Article
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institution Curtin University Malaysia
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last_indexed 2025-11-14T11:17:51Z
publishDate 2015
publisher Elsevier
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spelling curtin-20.500.11937-812602021-01-13T07:53:49Z A DNA barcoding approach to identify plant species in multiflower honey Caridi, L. Bruni, I. Galimberti, A. Scaccabarozzi, Daniela De Mattia, F. Casiraghi, M. Labra, M. The purpose of this study was to test the ability of DNA barcoding to identify the plant origins of processed honey. Four multifloral honeys produced at different sites in a floristically rich area in the northern Italian Alps were examined by using the rbcL and trnH-psbA plastid regions as barcode markers. An extensive reference database of barcode sequences was generated for the local flora to determine the taxonomic composition of honey. Thirty-nine plant species were identified in the four honey samples, each of which originated from a mix of common plants belonging to Castanea, Quercus, Fagus and several herbaceous taxa. Interestingly, at least one endemic plant was found in all four honey samples, providing a clear signature for the geographic identity of these products. DNA of the toxic plant Atropa belladonna was detected in one sample, illustrating the usefulness of DNA barcoding for evaluating the safety of honey. 2015 Journal Article http://hdl.handle.net/20.500.11937/81260 10.1016/j.foodchem.2014.08.060 Elsevier restricted
spellingShingle Caridi, L.
Bruni, I.
Galimberti, A.
Scaccabarozzi, Daniela
De Mattia, F.
Casiraghi, M.
Labra, M.
A DNA barcoding approach to identify plant species in multiflower honey
title A DNA barcoding approach to identify plant species in multiflower honey
title_full A DNA barcoding approach to identify plant species in multiflower honey
title_fullStr A DNA barcoding approach to identify plant species in multiflower honey
title_full_unstemmed A DNA barcoding approach to identify plant species in multiflower honey
title_short A DNA barcoding approach to identify plant species in multiflower honey
title_sort dna barcoding approach to identify plant species in multiflower honey
url http://hdl.handle.net/20.500.11937/81260