Constitutive Activation of RAS/MAPK Pathway Cooperates with Trisomy 21 and Is Therapeutically Exploitable in Down Syndrome B-cell Leukemia
©2020 American Association for Cancer Research. PURPOSE: Children with Down syndrome (constitutive trisomy 21) that develop acute lymphoblastic leukemia (DS-ALL) have a 3-fold increased likelihood of treatment-related mortality coupled with a higher cumulative incidence of relapse, compared with oth...
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| Format: | Journal Article |
| Language: | English |
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AMER ASSOC CANCER RESEARCH
2020
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| Online Access: | http://purl.org/au-research/grants/nhmrc/1142627 http://hdl.handle.net/20.500.11937/81080 |
| _version_ | 1848764313812074496 |
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| author | Laurent, A.P. Siret, A. Ignacimouttou, C. Panchal, K. Diop, M. Jenni, S. Tsai, Y.C. Roos-Weil, D. Aid, Z. Prade, N. Lagarde, S. Plassard, D. Pierron, G. Daudigeos, E. Lecluse, Y. Droin, N. Bornhauser, B.C. Cheung, Laurence Crispino, J.D. Gaudry, M. Bernard, O.A. Macintyre, E. Barin Bonnigal, C. Kotecha, Rishi Geoerger, B. Ballerini, P. Bourquin, J.P. Delabesse, E. Mercher, T. Malinge, S. |
| author_facet | Laurent, A.P. Siret, A. Ignacimouttou, C. Panchal, K. Diop, M. Jenni, S. Tsai, Y.C. Roos-Weil, D. Aid, Z. Prade, N. Lagarde, S. Plassard, D. Pierron, G. Daudigeos, E. Lecluse, Y. Droin, N. Bornhauser, B.C. Cheung, Laurence Crispino, J.D. Gaudry, M. Bernard, O.A. Macintyre, E. Barin Bonnigal, C. Kotecha, Rishi Geoerger, B. Ballerini, P. Bourquin, J.P. Delabesse, E. Mercher, T. Malinge, S. |
| author_sort | Laurent, A.P. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | ©2020 American Association for Cancer Research. PURPOSE: Children with Down syndrome (constitutive trisomy 21) that develop acute lymphoblastic leukemia (DS-ALL) have a 3-fold increased likelihood of treatment-related mortality coupled with a higher cumulative incidence of relapse, compared with other children with B-cell acute lymphoblastic leukemia (B-ALL). This highlights the lack of suitable treatment for Down syndrome children with B-ALL. EXPERIMENTAL DESIGN: To facilitate the translation of new therapeutic agents into clinical trials, we built the first preclinical cohort of patient-derived xenograft (PDX) models of DS-ALL, comprehensively characterized at the genetic and transcriptomic levels, and have proven its suitability for preclinical studies by assessing the efficacy of drug combination between the MEK inhibitor trametinib and conventional chemotherapy agents. RESULTS: Whole-exome and RNA-sequencing experiments revealed a high incidence of somatic alterations leading to RAS/MAPK pathway activation in our cohort of DS-ALL, as well as in other pediatric B-ALL presenting somatic gain of the chromosome 21 (B-ALL+21). In murine and human B-cell precursors, activated KRASG12D functionally cooperates with trisomy 21 to deregulate transcriptional networks that promote increased proliferation and self renewal, as well as B-cell differentiation blockade. Moreover, we revealed that inhibition of RAS/MAPK pathway activation using the MEK1/2 inhibitor trametinib decreased leukemia burden in several PDX models of B-ALL+21, and enhanced survival of DS-ALL PDX in combination with conventional chemotherapy agents such as vincristine. CONCLUSIONS: Altogether, using novel and suitable PDX models, this study indicates that RAS/MAPK pathway inhibition represents a promising strategy to improve the outcome of Down syndrome children with B-cell precursor leukemia. |
| first_indexed | 2025-11-14T11:17:23Z |
| format | Journal Article |
| id | curtin-20.500.11937-81080 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T11:17:23Z |
| publishDate | 2020 |
| publisher | AMER ASSOC CANCER RESEARCH |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-810802021-01-06T05:58:36Z Constitutive Activation of RAS/MAPK Pathway Cooperates with Trisomy 21 and Is Therapeutically Exploitable in Down Syndrome B-cell Leukemia Laurent, A.P. Siret, A. Ignacimouttou, C. Panchal, K. Diop, M. Jenni, S. Tsai, Y.C. Roos-Weil, D. Aid, Z. Prade, N. Lagarde, S. Plassard, D. Pierron, G. Daudigeos, E. Lecluse, Y. Droin, N. Bornhauser, B.C. Cheung, Laurence Crispino, J.D. Gaudry, M. Bernard, O.A. Macintyre, E. Barin Bonnigal, C. Kotecha, Rishi Geoerger, B. Ballerini, P. Bourquin, J.P. Delabesse, E. Mercher, T. Malinge, S. Science & Technology Life Sciences & Biomedicine Oncology ACUTE LYMPHOBLASTIC-LEUKEMIA GENOMIC LANDSCAPE CRITICAL REGION RAS PATHWAY JAK2 CHROMOSOME-21 EXPRESSION MUTATIONS CANCER CRLF2 ©2020 American Association for Cancer Research. PURPOSE: Children with Down syndrome (constitutive trisomy 21) that develop acute lymphoblastic leukemia (DS-ALL) have a 3-fold increased likelihood of treatment-related mortality coupled with a higher cumulative incidence of relapse, compared with other children with B-cell acute lymphoblastic leukemia (B-ALL). This highlights the lack of suitable treatment for Down syndrome children with B-ALL. EXPERIMENTAL DESIGN: To facilitate the translation of new therapeutic agents into clinical trials, we built the first preclinical cohort of patient-derived xenograft (PDX) models of DS-ALL, comprehensively characterized at the genetic and transcriptomic levels, and have proven its suitability for preclinical studies by assessing the efficacy of drug combination between the MEK inhibitor trametinib and conventional chemotherapy agents. RESULTS: Whole-exome and RNA-sequencing experiments revealed a high incidence of somatic alterations leading to RAS/MAPK pathway activation in our cohort of DS-ALL, as well as in other pediatric B-ALL presenting somatic gain of the chromosome 21 (B-ALL+21). In murine and human B-cell precursors, activated KRASG12D functionally cooperates with trisomy 21 to deregulate transcriptional networks that promote increased proliferation and self renewal, as well as B-cell differentiation blockade. Moreover, we revealed that inhibition of RAS/MAPK pathway activation using the MEK1/2 inhibitor trametinib decreased leukemia burden in several PDX models of B-ALL+21, and enhanced survival of DS-ALL PDX in combination with conventional chemotherapy agents such as vincristine. CONCLUSIONS: Altogether, using novel and suitable PDX models, this study indicates that RAS/MAPK pathway inhibition represents a promising strategy to improve the outcome of Down syndrome children with B-cell precursor leukemia. 2020 Journal Article http://hdl.handle.net/20.500.11937/81080 10.1158/1078-0432.CCR-19-3519 English http://purl.org/au-research/grants/nhmrc/1142627 AMER ASSOC CANCER RESEARCH restricted |
| spellingShingle | Science & Technology Life Sciences & Biomedicine Oncology ACUTE LYMPHOBLASTIC-LEUKEMIA GENOMIC LANDSCAPE CRITICAL REGION RAS PATHWAY JAK2 CHROMOSOME-21 EXPRESSION MUTATIONS CANCER CRLF2 Laurent, A.P. Siret, A. Ignacimouttou, C. Panchal, K. Diop, M. Jenni, S. Tsai, Y.C. Roos-Weil, D. Aid, Z. Prade, N. Lagarde, S. Plassard, D. Pierron, G. Daudigeos, E. Lecluse, Y. Droin, N. Bornhauser, B.C. Cheung, Laurence Crispino, J.D. Gaudry, M. Bernard, O.A. Macintyre, E. Barin Bonnigal, C. Kotecha, Rishi Geoerger, B. Ballerini, P. Bourquin, J.P. Delabesse, E. Mercher, T. Malinge, S. Constitutive Activation of RAS/MAPK Pathway Cooperates with Trisomy 21 and Is Therapeutically Exploitable in Down Syndrome B-cell Leukemia |
| title | Constitutive Activation of RAS/MAPK Pathway Cooperates with Trisomy 21 and Is Therapeutically Exploitable in Down Syndrome B-cell Leukemia |
| title_full | Constitutive Activation of RAS/MAPK Pathway Cooperates with Trisomy 21 and Is Therapeutically Exploitable in Down Syndrome B-cell Leukemia |
| title_fullStr | Constitutive Activation of RAS/MAPK Pathway Cooperates with Trisomy 21 and Is Therapeutically Exploitable in Down Syndrome B-cell Leukemia |
| title_full_unstemmed | Constitutive Activation of RAS/MAPK Pathway Cooperates with Trisomy 21 and Is Therapeutically Exploitable in Down Syndrome B-cell Leukemia |
| title_short | Constitutive Activation of RAS/MAPK Pathway Cooperates with Trisomy 21 and Is Therapeutically Exploitable in Down Syndrome B-cell Leukemia |
| title_sort | constitutive activation of ras/mapk pathway cooperates with trisomy 21 and is therapeutically exploitable in down syndrome b-cell leukemia |
| topic | Science & Technology Life Sciences & Biomedicine Oncology ACUTE LYMPHOBLASTIC-LEUKEMIA GENOMIC LANDSCAPE CRITICAL REGION RAS PATHWAY JAK2 CHROMOSOME-21 EXPRESSION MUTATIONS CANCER CRLF2 |
| url | http://purl.org/au-research/grants/nhmrc/1142627 http://hdl.handle.net/20.500.11937/81080 |