Refining trophic dynamics through multi-factor Bayesian mixing models: A case study of subterranean beetles
© 2020 The Authors. Ecology and Evolution published by John Wiley & Sons Ltd Food web dynamics are vital in shaping the functional ecology of ecosystems. However, trophic ecology is still in its infancy in groundwater ecosystems due to the cryptic nature of these environments. To unravel t...
| Main Authors: | , , , , , , , , , |
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| Format: | Journal Article |
| Language: | English |
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WILEY
2020
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| Online Access: | http://purl.org/au-research/grants/arc/LP140100555 http://hdl.handle.net/20.500.11937/80937 |
| _version_ | 1848764293653200896 |
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| author | Sacco, Mattia Blyth, Alison Humphreys, W.F. Cooper, S.J.B. Austin, A.D. Hyde, J. Mazumder, D. Hua, Q. White, Nicole Grice, Kliti |
| author_facet | Sacco, Mattia Blyth, Alison Humphreys, W.F. Cooper, S.J.B. Austin, A.D. Hyde, J. Mazumder, D. Hua, Q. White, Nicole Grice, Kliti |
| author_sort | Sacco, Mattia |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | © 2020 The Authors.
Ecology and Evolution published by John Wiley & Sons Ltd Food web dynamics are vital in shaping the functional ecology of ecosystems. However, trophic ecology is still in its infancy in groundwater ecosystems due to the cryptic nature of these environments. To unravel trophic interactions between subterranean biota, we applied an interdisciplinary Bayesian mixing model design (multi-factor BMM) based on the integration of faunal C and N bulk tissue stable isotope data (d13C and d15N) with radiocarbon data (?14C), and prior information from metagenomic analyses. We further compared outcomes from multi-factor BMM with a conventional isotope double proxy mixing model (SIA BMM), triple proxy (d13C, d15N, and ?14C, multi-proxy BMM), and double proxy combined with DNA prior information (SIA + DNA BMM) designs. Three species of subterranean beetles (Paroster macrosturtensis, Paroster mesosturtensis, and Paroster microsturtensis) and their main prey items Chiltoniidae amphipods (AM1: Scutachiltonia axfordi and AM2: Yilgarniella sturtensis), cyclopoids and harpacticoids from a calcrete in Western Australia were targeted. Diet estimations from stable isotope only models (SIA BMM) indicated homogeneous patterns with modest preferences for amphipods as prey items. Multi-proxy BMM suggested increased—and species-specific—predatory pressures on amphipods coupled with high rates of scavenging/predation on sister species. SIA + DNA BMM showed marked preferences for amphipods AM1 and AM2, and reduced interspecific scavenging/predation on Paroster species. Multi-factorial BMM revealed the most precise estimations (lower overall SD and very marginal beetles' interspecific interactions), indicating consistent preferences for amphipods AM1 in all the beetles' diets. Incorporation of genetic priors allowed crucial refining of the feeding preferences, while integration of more expensive radiocarbon data as a third proxy (when combined with genetic data) produced more precise outcomes but close dietary reconstruction to that from SIA + DNA BMM. Further multidisciplinary modeling from other groundwater environments will help elucidate the potential behind these designs and bring light to the feeding ecology of one the most vital ecosystems worldwide. |
| first_indexed | 2025-11-14T11:17:03Z |
| format | Journal Article |
| id | curtin-20.500.11937-80937 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T11:17:03Z |
| publishDate | 2020 |
| publisher | WILEY |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-809372021-01-05T08:07:07Z Refining trophic dynamics through multi-factor Bayesian mixing models: A case study of subterranean beetles Sacco, Mattia Blyth, Alison Humphreys, W.F. Cooper, S.J.B. Austin, A.D. Hyde, J. Mazumder, D. Hua, Q. White, Nicole Grice, Kliti Science & Technology Life Sciences & Biomedicine Ecology Evolutionary Biology Environmental Sciences & Ecology Bayesian mixing models food webs groundwater metagenomics radiocarbon stygofauna FOOD-WEB STRUCTURE STABLE-ISOTOPES CALCRETE AQUIFER DIVING BEETLES AMINO-ACIDS ARID ZONE ECOLOGY DNA RADIOCARBON DIVERSITY © 2020 The Authors. Ecology and Evolution published by John Wiley & Sons Ltd Food web dynamics are vital in shaping the functional ecology of ecosystems. However, trophic ecology is still in its infancy in groundwater ecosystems due to the cryptic nature of these environments. To unravel trophic interactions between subterranean biota, we applied an interdisciplinary Bayesian mixing model design (multi-factor BMM) based on the integration of faunal C and N bulk tissue stable isotope data (d13C and d15N) with radiocarbon data (?14C), and prior information from metagenomic analyses. We further compared outcomes from multi-factor BMM with a conventional isotope double proxy mixing model (SIA BMM), triple proxy (d13C, d15N, and ?14C, multi-proxy BMM), and double proxy combined with DNA prior information (SIA + DNA BMM) designs. Three species of subterranean beetles (Paroster macrosturtensis, Paroster mesosturtensis, and Paroster microsturtensis) and their main prey items Chiltoniidae amphipods (AM1: Scutachiltonia axfordi and AM2: Yilgarniella sturtensis), cyclopoids and harpacticoids from a calcrete in Western Australia were targeted. Diet estimations from stable isotope only models (SIA BMM) indicated homogeneous patterns with modest preferences for amphipods as prey items. Multi-proxy BMM suggested increased—and species-specific—predatory pressures on amphipods coupled with high rates of scavenging/predation on sister species. SIA + DNA BMM showed marked preferences for amphipods AM1 and AM2, and reduced interspecific scavenging/predation on Paroster species. Multi-factorial BMM revealed the most precise estimations (lower overall SD and very marginal beetles' interspecific interactions), indicating consistent preferences for amphipods AM1 in all the beetles' diets. Incorporation of genetic priors allowed crucial refining of the feeding preferences, while integration of more expensive radiocarbon data as a third proxy (when combined with genetic data) produced more precise outcomes but close dietary reconstruction to that from SIA + DNA BMM. Further multidisciplinary modeling from other groundwater environments will help elucidate the potential behind these designs and bring light to the feeding ecology of one the most vital ecosystems worldwide. 2020 Journal Article http://hdl.handle.net/20.500.11937/80937 10.1002/ece3.6580 English http://purl.org/au-research/grants/arc/LP140100555 http://creativecommons.org/licenses/by/4.0/ WILEY fulltext |
| spellingShingle | Science & Technology Life Sciences & Biomedicine Ecology Evolutionary Biology Environmental Sciences & Ecology Bayesian mixing models food webs groundwater metagenomics radiocarbon stygofauna FOOD-WEB STRUCTURE STABLE-ISOTOPES CALCRETE AQUIFER DIVING BEETLES AMINO-ACIDS ARID ZONE ECOLOGY DNA RADIOCARBON DIVERSITY Sacco, Mattia Blyth, Alison Humphreys, W.F. Cooper, S.J.B. Austin, A.D. Hyde, J. Mazumder, D. Hua, Q. White, Nicole Grice, Kliti Refining trophic dynamics through multi-factor Bayesian mixing models: A case study of subterranean beetles |
| title | Refining trophic dynamics through multi-factor Bayesian mixing models: A case study of subterranean beetles |
| title_full | Refining trophic dynamics through multi-factor Bayesian mixing models: A case study of subterranean beetles |
| title_fullStr | Refining trophic dynamics through multi-factor Bayesian mixing models: A case study of subterranean beetles |
| title_full_unstemmed | Refining trophic dynamics through multi-factor Bayesian mixing models: A case study of subterranean beetles |
| title_short | Refining trophic dynamics through multi-factor Bayesian mixing models: A case study of subterranean beetles |
| title_sort | refining trophic dynamics through multi-factor bayesian mixing models: a case study of subterranean beetles |
| topic | Science & Technology Life Sciences & Biomedicine Ecology Evolutionary Biology Environmental Sciences & Ecology Bayesian mixing models food webs groundwater metagenomics radiocarbon stygofauna FOOD-WEB STRUCTURE STABLE-ISOTOPES CALCRETE AQUIFER DIVING BEETLES AMINO-ACIDS ARID ZONE ECOLOGY DNA RADIOCARBON DIVERSITY |
| url | http://purl.org/au-research/grants/arc/LP140100555 http://hdl.handle.net/20.500.11937/80937 |