Genome-wide fitness analyses of the foodborne pathogen Campylobacter jejuni in in vitro and in vivo models
Campylobacter is the most common cause of foodborne bacterial illness worldwide. Faecal contamination of meat, especially chicken, during processing represents a key route of transmission to humans. There is a lack of insight into the mechanisms driving C. jejuni growth and survival within hosts and...
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| Format: | Journal Article |
| Language: | English |
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NATURE PUBLISHING GROUP
2017
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| Online Access: | http://hdl.handle.net/20.500.11937/80733 |
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| author | De Vries, S.P.W. Gupta, S. Baig, A. Wright, E. Wedley, A. Jensen, A.N. Lora, L.L. Humphrey, S. Skovgard, H. MacLeod, K. Pont, E. Wolanska, D.P. L'Heureux, J. Mobegi, Fredrick Smith, D.G.E. Everest, P. Zomer, A. Williams, N. Wigley, P. Humphrey, T. Maskel, D.J. Grant, A.J. |
| author_facet | De Vries, S.P.W. Gupta, S. Baig, A. Wright, E. Wedley, A. Jensen, A.N. Lora, L.L. Humphrey, S. Skovgard, H. MacLeod, K. Pont, E. Wolanska, D.P. L'Heureux, J. Mobegi, Fredrick Smith, D.G.E. Everest, P. Zomer, A. Williams, N. Wigley, P. Humphrey, T. Maskel, D.J. Grant, A.J. |
| author_sort | De Vries, S.P.W. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | Campylobacter is the most common cause of foodborne bacterial illness worldwide. Faecal contamination of meat, especially chicken, during processing represents a key route of transmission to humans. There is a lack of insight into the mechanisms driving C. jejuni growth and survival within hosts and the environment. Here, we report a detailed analysis of C. jejuni fitness across models reflecting stages in its life cycle. Transposon (Tn) gene-inactivation libraries were generated in three C. jejuni strains and the impact on fitness during chicken colonisation, survival in houseflies and under nutrient-rich and -poor conditions at 4 °C and infection of human gut epithelial cells was assessed by Tn-insertion site sequencing (Tn-seq). A total of 331 homologous gene clusters were essential for fitness during in vitro growth in three C. jejuni strains, revealing that a large part of its genome is dedicated to growth. We report novel C. jejuni factors essential throughout its life cycle. Importantly, we identified genes that fulfil important roles across multiple conditions. Our comprehensive screens showed which flagella elements are essential for growth and which are vital to the interaction with host organisms. Future efforts should focus on how to exploit this knowledge to effectively control infections caused by C. jejuni. |
| first_indexed | 2025-11-14T11:16:32Z |
| format | Journal Article |
| id | curtin-20.500.11937-80733 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T11:16:32Z |
| publishDate | 2017 |
| publisher | NATURE PUBLISHING GROUP |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-807332021-01-07T07:46:47Z Genome-wide fitness analyses of the foodborne pathogen Campylobacter jejuni in in vitro and in vivo models De Vries, S.P.W. Gupta, S. Baig, A. Wright, E. Wedley, A. Jensen, A.N. Lora, L.L. Humphrey, S. Skovgard, H. MacLeod, K. Pont, E. Wolanska, D.P. L'Heureux, J. Mobegi, Fredrick Smith, D.G.E. Everest, P. Zomer, A. Williams, N. Wigley, P. Humphrey, T. Maskel, D.J. Grant, A.J. Science & Technology Multidisciplinary Sciences Science & Technology - Other Topics ESCHERICHIA-COLI ENVIRONMENTAL SURVIVAL GASTROINTESTINAL-TRACT CHICKEN COLONIZATION DIPTERA MUSCIDAE EPITHELIAL-CELLS TRANSMISSION SEQUENCE STRAIN GENES Campylobacter is the most common cause of foodborne bacterial illness worldwide. Faecal contamination of meat, especially chicken, during processing represents a key route of transmission to humans. There is a lack of insight into the mechanisms driving C. jejuni growth and survival within hosts and the environment. Here, we report a detailed analysis of C. jejuni fitness across models reflecting stages in its life cycle. Transposon (Tn) gene-inactivation libraries were generated in three C. jejuni strains and the impact on fitness during chicken colonisation, survival in houseflies and under nutrient-rich and -poor conditions at 4 °C and infection of human gut epithelial cells was assessed by Tn-insertion site sequencing (Tn-seq). A total of 331 homologous gene clusters were essential for fitness during in vitro growth in three C. jejuni strains, revealing that a large part of its genome is dedicated to growth. We report novel C. jejuni factors essential throughout its life cycle. Importantly, we identified genes that fulfil important roles across multiple conditions. Our comprehensive screens showed which flagella elements are essential for growth and which are vital to the interaction with host organisms. Future efforts should focus on how to exploit this knowledge to effectively control infections caused by C. jejuni. 2017 Journal Article http://hdl.handle.net/20.500.11937/80733 10.1038/s41598-017-01133-4 English http://creativecommons.org/licenses/by/4.0/ NATURE PUBLISHING GROUP fulltext |
| spellingShingle | Science & Technology Multidisciplinary Sciences Science & Technology - Other Topics ESCHERICHIA-COLI ENVIRONMENTAL SURVIVAL GASTROINTESTINAL-TRACT CHICKEN COLONIZATION DIPTERA MUSCIDAE EPITHELIAL-CELLS TRANSMISSION SEQUENCE STRAIN GENES De Vries, S.P.W. Gupta, S. Baig, A. Wright, E. Wedley, A. Jensen, A.N. Lora, L.L. Humphrey, S. Skovgard, H. MacLeod, K. Pont, E. Wolanska, D.P. L'Heureux, J. Mobegi, Fredrick Smith, D.G.E. Everest, P. Zomer, A. Williams, N. Wigley, P. Humphrey, T. Maskel, D.J. Grant, A.J. Genome-wide fitness analyses of the foodborne pathogen Campylobacter jejuni in in vitro and in vivo models |
| title | Genome-wide fitness analyses of the foodborne pathogen Campylobacter jejuni in in vitro and in vivo models |
| title_full | Genome-wide fitness analyses of the foodborne pathogen Campylobacter jejuni in in vitro and in vivo models |
| title_fullStr | Genome-wide fitness analyses of the foodborne pathogen Campylobacter jejuni in in vitro and in vivo models |
| title_full_unstemmed | Genome-wide fitness analyses of the foodborne pathogen Campylobacter jejuni in in vitro and in vivo models |
| title_short | Genome-wide fitness analyses of the foodborne pathogen Campylobacter jejuni in in vitro and in vivo models |
| title_sort | genome-wide fitness analyses of the foodborne pathogen campylobacter jejuni in in vitro and in vivo models |
| topic | Science & Technology Multidisciplinary Sciences Science & Technology - Other Topics ESCHERICHIA-COLI ENVIRONMENTAL SURVIVAL GASTROINTESTINAL-TRACT CHICKEN COLONIZATION DIPTERA MUSCIDAE EPITHELIAL-CELLS TRANSMISSION SEQUENCE STRAIN GENES |
| url | http://hdl.handle.net/20.500.11937/80733 |