ROS in platelet biology: Functional aspects and methodological insights

© 2020 by the authors. Reactive oxygen species (ROS) and mitochondria play a pivotal role in regulating platelet functions. Platelet activation determines a drastic change in redox balance and in platelet metabolism. Indeed, several signaling pathways have been demonstrated to induce ROS product...

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Main Authors: Masselli, E., Pozzi, G., Vaccarezza, Mauro, Mirandola, P., Galli, D., Vitale, M., Carubbi, C., Gobbi, G.
Format: Journal Article
Language:English
Published: 2020
Subjects:
Online Access:http://hdl.handle.net/20.500.11937/80185
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author Masselli, E.
Pozzi, G.
Vaccarezza, Mauro
Mirandola, P.
Galli, D.
Vitale, M.
Carubbi, C.
Gobbi, G.
author_facet Masselli, E.
Pozzi, G.
Vaccarezza, Mauro
Mirandola, P.
Galli, D.
Vitale, M.
Carubbi, C.
Gobbi, G.
author_sort Masselli, E.
building Curtin Institutional Repository
collection Online Access
description © 2020 by the authors. Reactive oxygen species (ROS) and mitochondria play a pivotal role in regulating platelet functions. Platelet activation determines a drastic change in redox balance and in platelet metabolism. Indeed, several signaling pathways have been demonstrated to induce ROS production by NAPDH oxidase (NOX) and mitochondria, upon platelet activation. Platelet-derived ROS, in turn, boost further ROS production and consequent platelet activation, adhesion and recruitment in an auto-amplifying loop. This vicious circle results in a platelet procoagulant phenotype and apoptosis, both accounting for the high thrombotic risk in oxidative stress-related diseases. This review sought to elucidate molecular mechanisms underlying ROS production upon platelet activation and the effects of an altered redox balance on platelet function, focusing on the main advances that have been made in platelet redox biology. Furthermore, given the increasing interest in this field, we also describe the up-to-date methods for detecting platelets, ROS and the platelet bioenergetic profile, which have been proposed as potential disease biomarkers.
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spelling curtin-20.500.11937-801852021-01-05T08:07:08Z ROS in platelet biology: Functional aspects and methodological insights Masselli, E. Pozzi, G. Vaccarezza, Mauro Mirandola, P. Galli, D. Vitale, M. Carubbi, C. Gobbi, G. ROS mitochondria oxidative stress platelet © 2020 by the authors. Reactive oxygen species (ROS) and mitochondria play a pivotal role in regulating platelet functions. Platelet activation determines a drastic change in redox balance and in platelet metabolism. Indeed, several signaling pathways have been demonstrated to induce ROS production by NAPDH oxidase (NOX) and mitochondria, upon platelet activation. Platelet-derived ROS, in turn, boost further ROS production and consequent platelet activation, adhesion and recruitment in an auto-amplifying loop. This vicious circle results in a platelet procoagulant phenotype and apoptosis, both accounting for the high thrombotic risk in oxidative stress-related diseases. This review sought to elucidate molecular mechanisms underlying ROS production upon platelet activation and the effects of an altered redox balance on platelet function, focusing on the main advances that have been made in platelet redox biology. Furthermore, given the increasing interest in this field, we also describe the up-to-date methods for detecting platelets, ROS and the platelet bioenergetic profile, which have been proposed as potential disease biomarkers. 2020 Journal Article http://hdl.handle.net/20.500.11937/80185 10.3390/ijms21144866 eng http://creativecommons.org/licenses/by/4.0/ fulltext
spellingShingle ROS
mitochondria
oxidative stress
platelet
Masselli, E.
Pozzi, G.
Vaccarezza, Mauro
Mirandola, P.
Galli, D.
Vitale, M.
Carubbi, C.
Gobbi, G.
ROS in platelet biology: Functional aspects and methodological insights
title ROS in platelet biology: Functional aspects and methodological insights
title_full ROS in platelet biology: Functional aspects and methodological insights
title_fullStr ROS in platelet biology: Functional aspects and methodological insights
title_full_unstemmed ROS in platelet biology: Functional aspects and methodological insights
title_short ROS in platelet biology: Functional aspects and methodological insights
title_sort ros in platelet biology: functional aspects and methodological insights
topic ROS
mitochondria
oxidative stress
platelet
url http://hdl.handle.net/20.500.11937/80185