Characterisation of Insulin at the Interface Between Two Immiscible Electrolyte Solutions

The behaviour of insulin was characterised at the interface between the two immiscible electrolyte solutions (ITIES). Electroadsorbed insulin was subjected to study by Fourier transform infrared and Raman spectroscopy, to understand any secondary structure changes following electroadsorption. Follow...

Full description

Bibliographic Details
Main Author: Lamichhane, Hum Bahadur
Format: Thesis
Published: Curtin University 2019
Online Access:http://hdl.handle.net/20.500.11937/80108
_version_ 1848764164038721536
author Lamichhane, Hum Bahadur
author_facet Lamichhane, Hum Bahadur
author_sort Lamichhane, Hum Bahadur
building Curtin Institutional Repository
collection Online Access
description The behaviour of insulin was characterised at the interface between the two immiscible electrolyte solutions (ITIES). Electroadsorbed insulin was subjected to study by Fourier transform infrared and Raman spectroscopy, to understand any secondary structure changes following electroadsorption. Following electroadsorption, insulin showed secondary structure changes which varied with the aqueous insulin concentration. At higher concentration, significant proportions of combined ß-sheet and irregular helix structures were found, while a-helical forms were dominant at lower concentration. Raman spectroscopy was insensitive to insulin following electroadsorption.
first_indexed 2025-11-14T11:15:00Z
format Thesis
id curtin-20.500.11937-80108
institution Curtin University Malaysia
institution_category Local University
last_indexed 2025-11-14T11:15:00Z
publishDate 2019
publisher Curtin University
recordtype eprints
repository_type Digital Repository
spelling curtin-20.500.11937-801082020-07-22T02:06:31Z Characterisation of Insulin at the Interface Between Two Immiscible Electrolyte Solutions Lamichhane, Hum Bahadur The behaviour of insulin was characterised at the interface between the two immiscible electrolyte solutions (ITIES). Electroadsorbed insulin was subjected to study by Fourier transform infrared and Raman spectroscopy, to understand any secondary structure changes following electroadsorption. Following electroadsorption, insulin showed secondary structure changes which varied with the aqueous insulin concentration. At higher concentration, significant proportions of combined ß-sheet and irregular helix structures were found, while a-helical forms were dominant at lower concentration. Raman spectroscopy was insensitive to insulin following electroadsorption. 2019 Thesis http://hdl.handle.net/20.500.11937/80108 Curtin University fulltext
spellingShingle Lamichhane, Hum Bahadur
Characterisation of Insulin at the Interface Between Two Immiscible Electrolyte Solutions
title Characterisation of Insulin at the Interface Between Two Immiscible Electrolyte Solutions
title_full Characterisation of Insulin at the Interface Between Two Immiscible Electrolyte Solutions
title_fullStr Characterisation of Insulin at the Interface Between Two Immiscible Electrolyte Solutions
title_full_unstemmed Characterisation of Insulin at the Interface Between Two Immiscible Electrolyte Solutions
title_short Characterisation of Insulin at the Interface Between Two Immiscible Electrolyte Solutions
title_sort characterisation of insulin at the interface between two immiscible electrolyte solutions
url http://hdl.handle.net/20.500.11937/80108