Structural characterisation of the interaction between Triticum aestivum and the dothideomycete pathogen Stagonospora nodorum

The interaction between Stagonospora nodorum and a susceptible wheat cultivar was investigated using a range of microscopic techniques. Germination of pycnidiospores occurred approximately 3 h after making contact with the leaf surface and was followed by attempted penetration 8-12 h later. Penetrat...

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Main Authors: Solomon, P., Wilson, T., Rybak, K., Parker, K., Lowe, R., Oliver, Richard
Format: Journal Article
Published: Springer Netherlands 2006
Subjects:
Online Access:http://hdl.handle.net/20.500.11937/7966
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author Solomon, P.
Wilson, T.
Rybak, K.
Parker, K.
Lowe, R.
Oliver, Richard
author_facet Solomon, P.
Wilson, T.
Rybak, K.
Parker, K.
Lowe, R.
Oliver, Richard
author_sort Solomon, P.
building Curtin Institutional Repository
collection Online Access
description The interaction between Stagonospora nodorum and a susceptible wheat cultivar was investigated using a range of microscopic techniques. Germination of pycnidiospores occurred approximately 3 h after making contact with the leaf surface and was followed by attempted penetration 8-12 h later. Penetration was observed through stomata and also directly through periclinal and anticlinal epidermal cell walls. Penetration down the anticlinal cell walls appeared to occur without a differentiated penetrating structure whilst structures identified as either lateral appressoria or hyphopodia were typically present when penetrating over a periclinal cell wall. Once inside the leaf, the fungus continued to grow for the next 4-5 days colonising all parts of the leaf except the vascular bundles. Only in the later phase of the infection was total host cell collapse apparent. Evidence of polyphenolic compounds was observed. The infection cycle was completed within 7 days as indicated by sporulation on the leaf surface. These results have allowed us to understand how the fungus physically interacts with the leaf and will help the overall understanding of the infection process.
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spelling curtin-20.500.11937-79662019-05-21T07:33:06Z Structural characterisation of the interaction between Triticum aestivum and the dothideomycete pathogen Stagonospora nodorum Solomon, P. Wilson, T. Rybak, K. Parker, K. Lowe, R. Oliver, Richard STAINING TECHNIQUE FUNGI Stagonospora nodorum SEEDLING RESISTANCE leaf and glume blotch LEPTOSPHAERIA-MACULANS SEPTORIA-NODORUM hyphopodia MAGNAPORTHE-GRISEA WHEAT INFECTION MYCOSPHAERELLA-GRAMINICOLA LEAF BLOTCH LEAVES The interaction between Stagonospora nodorum and a susceptible wheat cultivar was investigated using a range of microscopic techniques. Germination of pycnidiospores occurred approximately 3 h after making contact with the leaf surface and was followed by attempted penetration 8-12 h later. Penetration was observed through stomata and also directly through periclinal and anticlinal epidermal cell walls. Penetration down the anticlinal cell walls appeared to occur without a differentiated penetrating structure whilst structures identified as either lateral appressoria or hyphopodia were typically present when penetrating over a periclinal cell wall. Once inside the leaf, the fungus continued to grow for the next 4-5 days colonising all parts of the leaf except the vascular bundles. Only in the later phase of the infection was total host cell collapse apparent. Evidence of polyphenolic compounds was observed. The infection cycle was completed within 7 days as indicated by sporulation on the leaf surface. These results have allowed us to understand how the fungus physically interacts with the leaf and will help the overall understanding of the infection process. 2006 Journal Article http://hdl.handle.net/20.500.11937/7966 10.1111/j.1365-2257.2006.00783.x Springer Netherlands restricted
spellingShingle STAINING TECHNIQUE
FUNGI
Stagonospora nodorum
SEEDLING RESISTANCE
leaf and glume blotch
LEPTOSPHAERIA-MACULANS
SEPTORIA-NODORUM
hyphopodia
MAGNAPORTHE-GRISEA
WHEAT INFECTION
MYCOSPHAERELLA-GRAMINICOLA
LEAF BLOTCH
LEAVES
Solomon, P.
Wilson, T.
Rybak, K.
Parker, K.
Lowe, R.
Oliver, Richard
Structural characterisation of the interaction between Triticum aestivum and the dothideomycete pathogen Stagonospora nodorum
title Structural characterisation of the interaction between Triticum aestivum and the dothideomycete pathogen Stagonospora nodorum
title_full Structural characterisation of the interaction between Triticum aestivum and the dothideomycete pathogen Stagonospora nodorum
title_fullStr Structural characterisation of the interaction between Triticum aestivum and the dothideomycete pathogen Stagonospora nodorum
title_full_unstemmed Structural characterisation of the interaction between Triticum aestivum and the dothideomycete pathogen Stagonospora nodorum
title_short Structural characterisation of the interaction between Triticum aestivum and the dothideomycete pathogen Stagonospora nodorum
title_sort structural characterisation of the interaction between triticum aestivum and the dothideomycete pathogen stagonospora nodorum
topic STAINING TECHNIQUE
FUNGI
Stagonospora nodorum
SEEDLING RESISTANCE
leaf and glume blotch
LEPTOSPHAERIA-MACULANS
SEPTORIA-NODORUM
hyphopodia
MAGNAPORTHE-GRISEA
WHEAT INFECTION
MYCOSPHAERELLA-GRAMINICOLA
LEAF BLOTCH
LEAVES
url http://hdl.handle.net/20.500.11937/7966