Characterization of Ionic and Lipid Gradients within Corpus Callosum White Matter after Diffuse Traumatic Brain Injury in the Rat
There is increased recognition of the effects of diffuse traumatic brain injury (dTBI), which can initiate yet unknown biochemical cascades, resulting in delayed secondary brain degeneration and long-term neurological sequela. There is limited availability of therapies that minimize the effect of se...
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| Format: | Journal Article |
| Language: | English |
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AMER CHEMICAL SOC
2020
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| Online Access: | http://purl.org/au-research/grants/nhmrc/1029311 http://hdl.handle.net/20.500.11937/79548 |
| _version_ | 1848764070537199616 |
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| author | Hartnell, David Gillespie-Jones, K. Ciornei, C. Hollings, Ashley Thomas, Alexander Harrild, Elizabeth Reinhardt, J. Paterson, D.J. Alwis, D. Rajan, R. Hackett, Mark |
| author_facet | Hartnell, David Gillespie-Jones, K. Ciornei, C. Hollings, Ashley Thomas, Alexander Harrild, Elizabeth Reinhardt, J. Paterson, D.J. Alwis, D. Rajan, R. Hackett, Mark |
| author_sort | Hartnell, David |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | There is increased recognition of the effects of diffuse traumatic brain injury (dTBI), which can initiate yet unknown biochemical cascades, resulting in delayed secondary brain degeneration and long-term neurological sequela. There is limited availability of therapies that minimize the effect of secondary brain damage on the quality of life of people who have suffered TBI, many of which were otherwise healthy adults. Understanding the cascade of biochemical events initiated in specific brain regions in the acute phase of dTBI and how this spreads into adjacent brain structures may provide the necessary insight into drive development of improved therapies. In this study, we have used direct biochemical imaging techniques (Fourier transform infrared spectroscopic imaging) and elemental mapping (X-ray fluorescence microscopy) to characterize biochemical and elemental alterations that occur in corpus callosum white matter in the acute phase of dTBI. The results provide direct visualization of differential biochemical and ionic changes that occur in the highly vulnerable medial corpus callosum white matter relative to the less vulnerable lateral regions of the corpus callosum. Specifically, the results suggest that altered ionic gradients manifest within mechanically damaged medial corpus callosum, potentially spreading to and inducing lipid alterations to white matter structures in lateral brain regions. |
| first_indexed | 2025-11-14T11:13:31Z |
| format | Journal Article |
| id | curtin-20.500.11937-79548 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T11:13:31Z |
| publishDate | 2020 |
| publisher | AMER CHEMICAL SOC |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-795482021-01-06T00:55:29Z Characterization of Ionic and Lipid Gradients within Corpus Callosum White Matter after Diffuse Traumatic Brain Injury in the Rat Hartnell, David Gillespie-Jones, K. Ciornei, C. Hollings, Ashley Thomas, Alexander Harrild, Elizabeth Reinhardt, J. Paterson, D.J. Alwis, D. Rajan, R. Hackett, Mark Science & Technology Life Sciences & Biomedicine Biochemistry & Molecular Biology Chemistry, Medicinal Neurosciences Pharmacology & Pharmacy Neurosciences & Neurology Brain injury ions white matter X-ray fluorescence synchrotron infrared MEMBRANE PHOSPHOLIPID DEGRADATION AXONAL INJURY ELEMENTAL COMPOSITION SUBSTANTIA-NIGRA IMPACT EPIDEMIOLOGY NEURONS PROTEIN PATHOPHYSIOLOGY COMPONENTS There is increased recognition of the effects of diffuse traumatic brain injury (dTBI), which can initiate yet unknown biochemical cascades, resulting in delayed secondary brain degeneration and long-term neurological sequela. There is limited availability of therapies that minimize the effect of secondary brain damage on the quality of life of people who have suffered TBI, many of which were otherwise healthy adults. Understanding the cascade of biochemical events initiated in specific brain regions in the acute phase of dTBI and how this spreads into adjacent brain structures may provide the necessary insight into drive development of improved therapies. In this study, we have used direct biochemical imaging techniques (Fourier transform infrared spectroscopic imaging) and elemental mapping (X-ray fluorescence microscopy) to characterize biochemical and elemental alterations that occur in corpus callosum white matter in the acute phase of dTBI. The results provide direct visualization of differential biochemical and ionic changes that occur in the highly vulnerable medial corpus callosum white matter relative to the less vulnerable lateral regions of the corpus callosum. Specifically, the results suggest that altered ionic gradients manifest within mechanically damaged medial corpus callosum, potentially spreading to and inducing lipid alterations to white matter structures in lateral brain regions. 2020 Journal Article http://hdl.handle.net/20.500.11937/79548 10.1021/acschemneuro.9b00257 English http://purl.org/au-research/grants/nhmrc/1029311 AMER CHEMICAL SOC fulltext |
| spellingShingle | Science & Technology Life Sciences & Biomedicine Biochemistry & Molecular Biology Chemistry, Medicinal Neurosciences Pharmacology & Pharmacy Neurosciences & Neurology Brain injury ions white matter X-ray fluorescence synchrotron infrared MEMBRANE PHOSPHOLIPID DEGRADATION AXONAL INJURY ELEMENTAL COMPOSITION SUBSTANTIA-NIGRA IMPACT EPIDEMIOLOGY NEURONS PROTEIN PATHOPHYSIOLOGY COMPONENTS Hartnell, David Gillespie-Jones, K. Ciornei, C. Hollings, Ashley Thomas, Alexander Harrild, Elizabeth Reinhardt, J. Paterson, D.J. Alwis, D. Rajan, R. Hackett, Mark Characterization of Ionic and Lipid Gradients within Corpus Callosum White Matter after Diffuse Traumatic Brain Injury in the Rat |
| title | Characterization of Ionic and Lipid Gradients within Corpus Callosum White Matter after Diffuse Traumatic Brain Injury in the Rat |
| title_full | Characterization of Ionic and Lipid Gradients within Corpus Callosum White Matter after Diffuse Traumatic Brain Injury in the Rat |
| title_fullStr | Characterization of Ionic and Lipid Gradients within Corpus Callosum White Matter after Diffuse Traumatic Brain Injury in the Rat |
| title_full_unstemmed | Characterization of Ionic and Lipid Gradients within Corpus Callosum White Matter after Diffuse Traumatic Brain Injury in the Rat |
| title_short | Characterization of Ionic and Lipid Gradients within Corpus Callosum White Matter after Diffuse Traumatic Brain Injury in the Rat |
| title_sort | characterization of ionic and lipid gradients within corpus callosum white matter after diffuse traumatic brain injury in the rat |
| topic | Science & Technology Life Sciences & Biomedicine Biochemistry & Molecular Biology Chemistry, Medicinal Neurosciences Pharmacology & Pharmacy Neurosciences & Neurology Brain injury ions white matter X-ray fluorescence synchrotron infrared MEMBRANE PHOSPHOLIPID DEGRADATION AXONAL INJURY ELEMENTAL COMPOSITION SUBSTANTIA-NIGRA IMPACT EPIDEMIOLOGY NEURONS PROTEIN PATHOPHYSIOLOGY COMPONENTS |
| url | http://purl.org/au-research/grants/nhmrc/1029311 http://hdl.handle.net/20.500.11937/79548 |