Imaging lipophilic regions in rodent brain tissue with halogenated BODIPY probes

The effect of halogen substitution in fluorescent BODIPY species was evaluated in the context of staining lipids in situ within brain tissue sections. Herein we demonstrate that the halogenated species maintain their known in vitro affinity when applied to detect lipids in situ in brain tissue secti...

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Main Authors: Hartnell, David, Schwehr, Bradley J, Gillespie-Jones, Kate, Alwis, Dasuni, Rajan, Ramesh, Hou, Huishu, Sylvain, Nicole J, Pushie, Michael J, Kelly, Michael E, Massi, Max, Hackett, Mark
Format: Journal Article
Language:English
Published: 2020
Online Access:http://purl.org/au-research/grants/arc/FT190100017
http://hdl.handle.net/20.500.11937/79546
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author Hartnell, David
Schwehr, Bradley J
Gillespie-Jones, Kate
Alwis, Dasuni
Rajan, Ramesh
Hou, Huishu
Sylvain, Nicole J
Pushie, Michael J
Kelly, Michael E
Massi, Max
Hackett, Mark
author_facet Hartnell, David
Schwehr, Bradley J
Gillespie-Jones, Kate
Alwis, Dasuni
Rajan, Ramesh
Hou, Huishu
Sylvain, Nicole J
Pushie, Michael J
Kelly, Michael E
Massi, Max
Hackett, Mark
author_sort Hartnell, David
building Curtin Institutional Repository
collection Online Access
description The effect of halogen substitution in fluorescent BODIPY species was evaluated in the context of staining lipids in situ within brain tissue sections. Herein we demonstrate that the halogenated species maintain their known in vitro affinity when applied to detect lipids in situ in brain tissue sections. Interestingly, the chlorine substituted compound revealed the highest specificify for white matter lipids. Furthermore, the halogen substituted compounds rapidly detected lipid enriched cells, in situ, associated with a case of brain pathology and neuroinflammation.
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institution Curtin University Malaysia
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language eng
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publishDate 2020
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spelling curtin-20.500.11937-795462023-02-02T03:24:10Z Imaging lipophilic regions in rodent brain tissue with halogenated BODIPY probes Hartnell, David Schwehr, Bradley J Gillespie-Jones, Kate Alwis, Dasuni Rajan, Ramesh Hou, Huishu Sylvain, Nicole J Pushie, Michael J Kelly, Michael E Massi, Max Hackett, Mark The effect of halogen substitution in fluorescent BODIPY species was evaluated in the context of staining lipids in situ within brain tissue sections. Herein we demonstrate that the halogenated species maintain their known in vitro affinity when applied to detect lipids in situ in brain tissue sections. Interestingly, the chlorine substituted compound revealed the highest specificify for white matter lipids. Furthermore, the halogen substituted compounds rapidly detected lipid enriched cells, in situ, associated with a case of brain pathology and neuroinflammation. 2020 Journal Article http://hdl.handle.net/20.500.11937/79546 10.1039/d0an00099j eng http://purl.org/au-research/grants/arc/FT190100017 restricted
spellingShingle Hartnell, David
Schwehr, Bradley J
Gillespie-Jones, Kate
Alwis, Dasuni
Rajan, Ramesh
Hou, Huishu
Sylvain, Nicole J
Pushie, Michael J
Kelly, Michael E
Massi, Max
Hackett, Mark
Imaging lipophilic regions in rodent brain tissue with halogenated BODIPY probes
title Imaging lipophilic regions in rodent brain tissue with halogenated BODIPY probes
title_full Imaging lipophilic regions in rodent brain tissue with halogenated BODIPY probes
title_fullStr Imaging lipophilic regions in rodent brain tissue with halogenated BODIPY probes
title_full_unstemmed Imaging lipophilic regions in rodent brain tissue with halogenated BODIPY probes
title_short Imaging lipophilic regions in rodent brain tissue with halogenated BODIPY probes
title_sort imaging lipophilic regions in rodent brain tissue with halogenated bodipy probes
url http://purl.org/au-research/grants/arc/FT190100017
http://hdl.handle.net/20.500.11937/79546