Investigation of a solvent-cast organogel to form a liquid-gel microinterface array for electrochemical detection of lysozyme
Ion transfer at aqueous-organogel interfaces enables the non-redox detection of ions and ionisable species by voltammetry. In this study, a non-thermal method for preparation of an organogel was employed and used for the detection of hen-egg-white-lysozyme (HEWL) via adsorptive stripping voltammetry...
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| Format: | Journal Article |
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Elsevier
2015
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| Online Access: | http://hdl.handle.net/20.500.11937/79099 |
| _version_ | 1848764000402145280 |
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| author | Felisilda, Bren Alvarez De Eulate, Eva Arrigan, Damien |
| author_facet | Felisilda, Bren Alvarez De Eulate, Eva Arrigan, Damien |
| author_sort | Felisilda, Bren |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | Ion transfer at aqueous-organogel interfaces enables the non-redox detection of ions and ionisable species by voltammetry. In this study, a non-thermal method for preparation of an organogel was employed and used for the detection of hen-egg-white-lysozyme (HEWL) via adsorptive stripping voltammetry at an array of aqueous-organogel microinterfaces. Tetrahydrofuran solvent casting was employed to prepare the organogel mixture, hence removing the need for heating of the solution to be gelled, as used in previous studies. Cyclic voltammetry of HEWL at the microinterface array revealed a broad adsorption process on the forward scan, at positive applied potentials, followed by a desorption peak at ca. 0.68 V, indicating the detection of HEWL in this region. Application of an adsorption step, where a constant optimized potential of 0.95 V was applied, followed by voltammetric detection provided for a linear response range of 0.02-0.84 μM and a detection limit of 0.030 μM for 300 s adsorption. The detection limit was further improved by utilizing differential pulse stripping voltammetry, resulting in detection limits of 0.017 μM, 0.014 μM, and 0.010 μM for adsorptive pre-concentration times of 60, 120 and 300 s, respectively, in unstirred solutions. These results are an improvement over other methods for the detection of HEWL at aqueous-organic interfaces and offers a basis for the label-free detection of protein. |
| first_indexed | 2025-11-14T11:12:24Z |
| format | Journal Article |
| id | curtin-20.500.11937-79099 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T11:12:24Z |
| publishDate | 2015 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-790992020-08-07T01:05:42Z Investigation of a solvent-cast organogel to form a liquid-gel microinterface array for electrochemical detection of lysozyme Felisilda, Bren Alvarez De Eulate, Eva Arrigan, Damien ITIES; Lysozyme; Solvent-cast organogel; Adsorption; Voltammetry; Protein Ion transfer at aqueous-organogel interfaces enables the non-redox detection of ions and ionisable species by voltammetry. In this study, a non-thermal method for preparation of an organogel was employed and used for the detection of hen-egg-white-lysozyme (HEWL) via adsorptive stripping voltammetry at an array of aqueous-organogel microinterfaces. Tetrahydrofuran solvent casting was employed to prepare the organogel mixture, hence removing the need for heating of the solution to be gelled, as used in previous studies. Cyclic voltammetry of HEWL at the microinterface array revealed a broad adsorption process on the forward scan, at positive applied potentials, followed by a desorption peak at ca. 0.68 V, indicating the detection of HEWL in this region. Application of an adsorption step, where a constant optimized potential of 0.95 V was applied, followed by voltammetric detection provided for a linear response range of 0.02-0.84 μM and a detection limit of 0.030 μM for 300 s adsorption. The detection limit was further improved by utilizing differential pulse stripping voltammetry, resulting in detection limits of 0.017 μM, 0.014 μM, and 0.010 μM for adsorptive pre-concentration times of 60, 120 and 300 s, respectively, in unstirred solutions. These results are an improvement over other methods for the detection of HEWL at aqueous-organic interfaces and offers a basis for the label-free detection of protein. 2015 Journal Article http://hdl.handle.net/20.500.11937/79099 10.1016/j.aca.2015.08.024 Elsevier fulltext |
| spellingShingle | ITIES; Lysozyme; Solvent-cast organogel; Adsorption; Voltammetry; Protein Felisilda, Bren Alvarez De Eulate, Eva Arrigan, Damien Investigation of a solvent-cast organogel to form a liquid-gel microinterface array for electrochemical detection of lysozyme |
| title | Investigation of a solvent-cast organogel to form a liquid-gel microinterface array for electrochemical detection of lysozyme |
| title_full | Investigation of a solvent-cast organogel to form a liquid-gel microinterface array for electrochemical detection of lysozyme |
| title_fullStr | Investigation of a solvent-cast organogel to form a liquid-gel microinterface array for electrochemical detection of lysozyme |
| title_full_unstemmed | Investigation of a solvent-cast organogel to form a liquid-gel microinterface array for electrochemical detection of lysozyme |
| title_short | Investigation of a solvent-cast organogel to form a liquid-gel microinterface array for electrochemical detection of lysozyme |
| title_sort | investigation of a solvent-cast organogel to form a liquid-gel microinterface array for electrochemical detection of lysozyme |
| topic | ITIES; Lysozyme; Solvent-cast organogel; Adsorption; Voltammetry; Protein |
| url | http://hdl.handle.net/20.500.11937/79099 |