Gene editing the phytoene desaturase alleles of Cavendish banana using CRISPR/Cas9
Bananas are a staple food source and a major export commodity worldwide. The Cavendish dessert banana is a triploid AAA genome type and accounts for around 47% of global production. Being essentially sterile, genetic modification is perhaps the only pathway available to improve this cultivar. In thi...
| Main Authors: | , , , , , , |
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| Format: | Journal Article |
| Language: | English |
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SPRINGER
2018
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| Online Access: | http://hdl.handle.net/20.500.11937/78229 |
| _version_ | 1848763943959396352 |
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| author | Eftekhari, Fatima Dugdale, B. Kleidon, J. Brinin, A. Shand, K. Waterhouse, P. Dale, J. |
| author_facet | Eftekhari, Fatima Dugdale, B. Kleidon, J. Brinin, A. Shand, K. Waterhouse, P. Dale, J. |
| author_sort | Eftekhari, Fatima |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | Bananas are a staple food source and a major export commodity worldwide. The Cavendish dessert banana is a triploid AAA genome type and accounts for around 47% of global production. Being essentially sterile, genetic modification is perhaps the only pathway available to improve this cultivar. In this study, we used the CRISPR/Cas9 gene editing system to deliver a self-cleaving polycistronic guide RNA (gRNA) designed to target exon 1 of the Phytoene desaturase (PDS) gene in the Cavendish cultivar “Williams”. Genotyping of 19 independent events showed a 100% PDS modification rate primarily in the form of insertions (1–105 nt) or deletions (1–55 nt) (indels) at the predicted cleavage site. Tri-allelic disruptive modifications were observed in 63% of plants and resulted in both albinism and dwarfing. Pale green (16%) and wildtype green (21%) phenotypes generally correlated with in-frame indels in at least one of the three PDS alleles. Editing efficiency was dependent on both target site selection and Cas9 abundance. This is the first report of a highly effective CRISPR/Cas9 modification system using a polycistronic gRNA in Cavendish banana. Such an editing platform will be of considerable utility for the development of disease resistance and novel agro-traits in this commercially important cultivar into the future. |
| first_indexed | 2025-11-14T11:11:30Z |
| format | Journal Article |
| id | curtin-20.500.11937-78229 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T11:11:30Z |
| publishDate | 2018 |
| publisher | SPRINGER |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-782292020-06-08T00:23:56Z Gene editing the phytoene desaturase alleles of Cavendish banana using CRISPR/Cas9 Eftekhari, Fatima Dugdale, B. Kleidon, J. Brinin, A. Shand, K. Waterhouse, P. Dale, J. Science & Technology Life Sciences & Biomedicine Biochemical Research Methods Biochemistry & Molecular Biology Biotechnology & Applied Microbiology Genome editing PDS Cavendish banana CRISPR/Cas9 SYSTEM GENOME PLANTS RNAS Bananas are a staple food source and a major export commodity worldwide. The Cavendish dessert banana is a triploid AAA genome type and accounts for around 47% of global production. Being essentially sterile, genetic modification is perhaps the only pathway available to improve this cultivar. In this study, we used the CRISPR/Cas9 gene editing system to deliver a self-cleaving polycistronic guide RNA (gRNA) designed to target exon 1 of the Phytoene desaturase (PDS) gene in the Cavendish cultivar “Williams”. Genotyping of 19 independent events showed a 100% PDS modification rate primarily in the form of insertions (1–105 nt) or deletions (1–55 nt) (indels) at the predicted cleavage site. Tri-allelic disruptive modifications were observed in 63% of plants and resulted in both albinism and dwarfing. Pale green (16%) and wildtype green (21%) phenotypes generally correlated with in-frame indels in at least one of the three PDS alleles. Editing efficiency was dependent on both target site selection and Cas9 abundance. This is the first report of a highly effective CRISPR/Cas9 modification system using a polycistronic gRNA in Cavendish banana. Such an editing platform will be of considerable utility for the development of disease resistance and novel agro-traits in this commercially important cultivar into the future. 2018 Journal Article http://hdl.handle.net/20.500.11937/78229 10.1007/s11248-018-0083-0 English http://creativecommons.org/licenses/by/4.0/ SPRINGER fulltext |
| spellingShingle | Science & Technology Life Sciences & Biomedicine Biochemical Research Methods Biochemistry & Molecular Biology Biotechnology & Applied Microbiology Genome editing PDS Cavendish banana CRISPR/Cas9 SYSTEM GENOME PLANTS RNAS Eftekhari, Fatima Dugdale, B. Kleidon, J. Brinin, A. Shand, K. Waterhouse, P. Dale, J. Gene editing the phytoene desaturase alleles of Cavendish banana using CRISPR/Cas9 |
| title | Gene editing the phytoene desaturase alleles of Cavendish banana using CRISPR/Cas9 |
| title_full | Gene editing the phytoene desaturase alleles of Cavendish banana using CRISPR/Cas9 |
| title_fullStr | Gene editing the phytoene desaturase alleles of Cavendish banana using CRISPR/Cas9 |
| title_full_unstemmed | Gene editing the phytoene desaturase alleles of Cavendish banana using CRISPR/Cas9 |
| title_short | Gene editing the phytoene desaturase alleles of Cavendish banana using CRISPR/Cas9 |
| title_sort | gene editing the phytoene desaturase alleles of cavendish banana using crispr/cas9 |
| topic | Science & Technology Life Sciences & Biomedicine Biochemical Research Methods Biochemistry & Molecular Biology Biotechnology & Applied Microbiology Genome editing PDS Cavendish banana CRISPR/Cas9 SYSTEM GENOME PLANTS RNAS |
| url | http://hdl.handle.net/20.500.11937/78229 |